23 research outputs found
Pushing the envelope in tissue engineering: Ex vivo production of thick vascularized cardiac extracellular matrix constructs
Functional vascularization is a prerequisite for cardiac tissue engineering of constructs with physiological thicknesses. We previously reported the successful preservation of main vascular conduits in isolated thick acellular porcine cardiac ventricular ECM (pcECM). We now unveil this scaffold's potential in supporting human cardiomyocytes and promoting new blood vessel development ex vivo, providing long-term cell support in the construct bulk. A custom-designed perfusion bioreactor was developed to remodel such vascularization ex vivo, demonstrating, for the first time, functional angiogenesis in vitro with various stages of vessel maturation supporting up to 1.7 mm thick constructs. A robust methodology was developed to assess the pcECM maximal cell capacity, which resembled the human heart cell density. Taken together these results demonstrate feasibility of producing physiological-like constructs such as the thick pcECM suggested here as a prospective treatment for end-stage heart failure. Methodologies reported herein may also benefit other tissues, offering a valuable in vitro setting for "thick-tissue" engineering strategies toward large animal in vivo studies.Israeli Science Foundation/1563/10Singapore National Research Foundatio
A discriminant analysis of neuropsychological effect of low lead exposure
Toxicology492-3309-314TXCY
Efficacy of phototherapy in neonatal hyperbilirubinaemia associated with glucose-6-phosphate dehydrogenase deficient status
European Journal of Pediatrics1527601-604EJPE
Efficacy of phototherapy in non-haemolytic hyperbilirubinaemia
British Medical Journal29365581361-1363BMJO
Clinical experience with phototherapy.
Annals of the Academy of Medicine Singapore18143-4
Neuropsychological studies on lead workers in Singapore
British Journal of Industrial Medicine439626-629BJIM
Effects of occupational exposure to toluene: A neuropsychological study on workers in Singapore
Annals of the Academy of Medicine Singapore262184-187AAMS
Psychological aspects of patients on haemodialysis
Singapore Medical Journal263259-261SIMJ