New Strategies for Implementing Locally Integrated Stream Restoration Projects.

Flooding risks in the Netherlands have been a high priority for decades because of its high population density, proximity to the North Sea, and extensive use of land reclamation techniques. With confounding factors such as climate and land-use changes, the Dutch water boards, governments, nongovernment organizations (NGOs), local farmers, and private citizens are addressing the vulnerabilities related to increasing flood risks by improving the resilience of the natural and social systems. Based on an existing framework of important governance regime characteristics, we explore how local groups work toward a more sustainable situation by producing synergetic win-win situations, constructive and cooperative planning and implementation, and the development of a high level of trus

Self-interference fluorescence microscopy: three dimensional fluorescence imaging without depth scanning

We present a new method for high-resolution, three-dimensional fluorescence imaging. In contrast to beam-scanning confocal microscopy, where the laser focus must be scanned both laterally and axially to collect a volume, we obtain depth information without the necessity of depth scanning. In this method, the emitted fluorescence is collected in the backward direction and is sent through a phase plate that encodes the depth information into the phase of a spectrally resolved interference pattern. We demonstrate that decoding this phase information allows for depth localization accuracy better than 4 μm over a 500 μm depth-of-field. In a high numerical aperture configuration with a much smaller depth of field, a localization accuracy of tens of nanometers can be achieved. This approach is ideally suited for miniature endoscopes, where space limitations at the endoscope tip render depth scanning difficult. We illustrate the potential for 3D visualization of complex biological samples by constructing a threedimensional volume of the microvasculature of ex vivo murine heart tissue from a single 2D scan. © 2012 Optical Society of America