Subway and its powerful presence in social media

“Analyzing Subway’s Successful Social Media Efforts” is a required project for the social media and marketing communications course. This project, which began during the fall semester of 2014, involves identifying Subway’s varying advertisement campaigns, the main target market these ads are intended for, the main media vehicles used to deliver these ads, and how effective these ads are in expanding Subway’s global footprint. More specifically, the research paper uncovers Subway’s varying advertisement campaign themes, multiple execution styles, and how Subway successfully applies the dual adaptation strategy in global markets through the use of social media formats Facebook and Twitter. The research also highlights the company’s history, main competitor, product lines and assortment, and its recent financial success. The paper concludes that Doctor’s Association’s Subway brand is highly successful in marketing its products due to its ability to relate the themes of health and fitness with its submarine sandwiches in ads that vary in both execution style and in media format based on the respective audience the company is trying to target. (Author abstract)Bodi, E. (2015). Analyzing Subway’s successful social media efforts. Retrieved from http://academicarchive.snhu.ed

Argumentative essay: Appearance discrimination in politics

The purpose of the research paper is to inform the reader of the impact a political candidate’s appearance has on his or her electoral success. The author cites sources that confirm role-independent traits (appearance, personality, other non-political traits) play a more crucial role in people’s voting decisions compared to the role-relevant traits (experience, political record, proposed plans) of politicians. The research paper cites evidence that proves role-independent traits are more important to voters by referencing research that explains how quickly individuals subconsciously identify the winner of an election after watching 10-second silent clips by observing candidate body language. The race and gender of politicians also influences the perceptions of voters, as voters have been proven to identify and relate different characteristics to different races and genders while other factors are held constant. Research also proves that certain appearance features like babyface and posture can either benefit or harm certain candidates depending on the circumstances. Finally, various media outlets like Facebook and YouTube influence people’s perceptions about political candidates in different ways. In conclusion, appearance discrimination in politics is a very complex topic, and it takes place in several different ways. Not much research has been done on why this takes place, but there is enough evidence in previous research that allows the author of this paper to conclude that people do vote for political candidates based their respective appearances. (Author abstract)Bodi, E. (2014). Appearance discrimination in politics. Retrieved from http://academicarchive.snhu.ed

A Genome-Wide Approach to Discovery of Small RNAs Involved in Regulation of Virulence in Vibrio cholerae

Small RNAs (sRNAs) are becoming increasingly recognized as important regulators in bacteria. To investigate the contribution of sRNA mediated regulation to virulence in Vibrio cholerae, we performed high throughput sequencing of cDNA generated from sRNA transcripts isolated from a strain ectopically expressing ToxT, the major transcriptional regulator within the virulence gene regulon. We compared this data set with ToxT binding sites determined by pulldown and deep sequencing to identify sRNA promoters directly controlled by ToxT. Analysis of the resulting transcripts with ToxT binding sites in cis revealed two sRNAs within the Vibrio Pathogenicity Island. When deletions of these sRNAs were made and the resulting strains were competed against the parental strain in the infant mouse model of V. cholerae colonization, one, TarB, displayed a variable colonization phenotype dependent on its physiological state at the time of inoculation. We identified a target of TarB as the mRNA for the secreted colonization factor, TcpF. We verified negative regulation of TcpF expression by TarB and, using point mutations that disrupted interaction between TarB and tpcF mRNA, showed that loss of this negative regulation was primarily responsible for the colonization phenotype observed in the TarB deletion mutant

A Wipe-Based Stool Collection and Preservation Kit for Microbiome Community Profiling.

While a range of methods for stool collection exist, many require complicated, self-directed protocols and stool transfer. In this study, we introduce and validate a novel, wipe-based approach to fecal sample collection and stabilization for metagenomics analysis. A total of 72 samples were collected across four different preservation types: freezing at -20°C, room temperature storage, a commercial DNA preservation kit, and a dissolvable wipe used with DESS (dimethyl sulfoxide, ethylenediaminetetraacetic acid, sodium chloride) solution. These samples were sequenced and analyzed for taxonomic abundance metrics, bacterial metabolic pathway classification, and diversity analysis. Overall, the DESS wipe results validated the use of a wipe-based capture method to collect stool samples for microbiome analysis, showing an R2 of 0.96 for species across all kingdoms, as well as exhibiting a maintenance of Shannon diversity (3.1-3.3) and species richness (151-159) compared to frozen samples. Moreover, DESS showed comparable performance to the commercially available preservation kit (R2 of 0.98), and samples consistently clustered by subject across each method. These data support that the DESS wipe method can be used for stable, room temperature collection and transport of human stool specimens