Sperimentazione di un nuovo antiossidante topico a base di Furfuril palmitato nel trattamento delle dermatiti eczematose del bambino e del neonato. Risultati di uno studio clinico multicentrico

In questo lavoro vengono presentati i risultati preliminari di uno studio clinico policentrico condotto su una preparazione topica (Triderm Lenil+) contenente Vit E, Superossido dismutasi, Acido 18 Glicirretico, alfa bisabololo e una nuova molecola antiossidante brevettata, il Furfuril palmitato. Questi principi attivi nel complesso proteggono la cute dai danni cellulari mediati dai radicali liberi che promuovono la sindrome infiammatoria. La loro azione, inoltre, è integrata dall'aggiunta di agenti in grado di riparare la barriera cutanea, quali fitosfingosina e fitosteroli. Il Furfuril palmitato, un estere ottenuto per reazione dell'alcool furfurilico con l'acido palmitico, ha un'elevata permeabilità cutanea ed è dotato di spiccate proprietà di quenching dell'ossigeno singoletto (102), agente reattivo coinvolto nella genesi di affezioni dermatologiche quali dermatite da contatto, dermatite atopica, psoriasi ed eritema solare. Nello studio in oggetto sono state indagate l'efficacia e la tollerabilità del prodotto applicato 2 volte al dì per 2 settimane in 60 pazienti pediatrici di età compresa tra 2 mesi e 14 anni, affetti in maggior parte da dermatite atopica e dermatite irritativa. Sono state riscontrate riduzioni significative della flogosi e della xerosi, con un evidente e rapido effetto decongestionante e lenitivo nella quasi totalità dei pazienti trattati, indipendentemente dal tipo di patologia. Il prodotto, privo di effetti collaterali e formulato in modo da evitare ogni rischio di sensibilizzazione, appare particolarmente indicato nel trattamento delle dermatiti pediatriche caratterizzate da quadri desquamativi, xerotici e eczematosi, laddove sia necessario un effetto lenitivo e contemporaneamente un reintegro della funzionalità della barriera cutane

Comparison of the Cytotoxic Potential of Cigarette Smoke and Electronic Cigarette Vapour Extract on Cultured Myocardial Cells

Background: Electronic cigarettes (ECs) have been marketed as an alternative-to-smoking habit. Besides chemical studies of the content of EC liquids or vapour, little research has been conducted on their in vitro effects. Smoking is an important risk factor for cardiovascular disease and cigarette smoke (CS) has well-established cytotoxic effects on myocardial cells. The purpose of this study was to evaluate the cytotoxic potential of the vapour of 20 EC liquid samples and a “base” liquid sample (50% glycerol and 50% propylene glycol, with no nicotine or flavourings) on cultured myocardial cells. Included were 4 samples produced by using cured tobacco leaves in order to extract the tobacco flavour. Methods: Cytotoxicity was tested according to the ISO 10993-5 standard. By activating an EC device at 3.7 volts (6.2 watts—all samples, including the “base” liquid) and at 4.5 volts (9.2 watts—four randomly selected samples), 200 mg of liquid evaporated and was extracted in 20 mL of culture medium. Cigarette smoke (CS) extract from three tobacco cigarettes was produced according to ISO 3308 method (2 s puffs of 35 mL volume, one puff every 60 s). The extracts, undiluted (100%) and in four dilutions (50%, 25%, 12.5%, and 6.25%), were applied to myocardial cells (H9c2); percent-viability was measured after 24 h incubation. According to ISO 10993-5, viability of <70% was considered cytotoxic. Results: CS extract was cytotoxic at extract concentrations >6.25% (viability: 76.9 ± 2.0% at 6.25%, 38.2 ± 0.5% at 12.5%, 3.1 ± 0.2% at 25%, 5.2 ± 0.8% at 50%, and 3.9 ± 0.2% at 100% extract concentration). Three EC extracts (produced by tobacco leaves) were cytotoxic at 100% and 50% extract concentrations (viability range: 2.2%–39.1% and 7.4%–66.9% respectively) and one (“Cinnamon-Cookies” flavour) was cytotoxic at 100% concentration only (viability: 64.8 ± 2.5%). Inhibitory concentration 50 was >3 times lower in CS extract compared to the worst-performing EC vapour extract. For EC extracts produced by high-voltage and energy, viability was reduced but no sample was cytotoxic according to ISO 10993-5 definition. Vapour produced by the “base” liquid was not cytotoxic at any extract concentration. Cell survival was not associated with nicotine concentration of EC liquids. Conclusions: This study indicates that some EC samples have cytotoxic properties on cultured cardiomyoblasts, associated with the production process and materials used in flavourings. However, all EC vapour extracts were significantly less cytotoxic compared to CS extract

Hepatocyte growth factor is a potent angiogenic factor which stimulates endothelial cell motility and growth.

10Hepatocyte Growth Factor (HGF, also known as Scatter Factor) is a powerful mitogen or motility factor in different cells, acting through the tyrosine kinase receptor encoded by the MET protooncogene. Endothelial cells express the MET gene and expose at the cell surface the mature protein (p190MET) made of a 50 kD (alpha) subunit disulfide linked to a 145-kD (beta) subunit. HGF binding to endothelial cells identifies two sites with different affinities. The higher affinity binding site (Kd = 0.35 nM) corresponds to the p190MET receptor. Sub-nanomolar concentrations of HGF, but not of a recombinant inactive precursor, stimulate the receptor kinase activity, cell proliferation and motility. HGF induces repairs of a wound in endothelial cell monolayer. HGF stimulates the scatter of endothelial cells grown on three-dimensional collagen gels, inducing an elongated phenotype. In the rabbit cornea, highly purified HGF promotes neovascularization at sub-nanomolar concentrations. HGF lacks activities related to hemostasis-thrombosis, inflammation and endothelial cells accessory functions. These data show that HGF is an in vivo potent angiogenic factor and in vitro induces endothelial cells to proliferate and migrate.reservedmixedF. Bussolino;M. F. Di;M. Ziche;E. Bocchietto;M. Olivero;L. Naldini;G. Gaudino;L. Tamagnone;A. Coffer;P. M. ComoglioF., Bussolino; M. F., Di; Ziche, Marina; E., Bocchietto; M., Olivero; L., Naldini; G., Gaudino; L., Tamagnone; A., Coffer; P. M., Comogli