Detection of Adhesion Genes and Slim Production among Staphylococcus Aureus and Staphylococcus Epidermidis Isolated from Hemodialysis Patients

The presence of the ica loci and adhesins genes in clinical Staphylococcus aureus and Staphylococcus epidermidis strains were considered important factors of virulence. In this study, one isolate of S. epidermidis and six isolates of S. aureus were isolated from cases of septicemia in hemodialysis patients and were investigated for slime production using Congo red agar methods (CRA). Biofilm production of S. epidermidis and S. aureus by using (CRA) plate revealed that all seven isolates were slime producers. In order to detect the adhesion genes (clf A, fnb A and cna) and presence of slime genes       (ica A and ica D) genes polymerase chain reaction was used. All seven isolates were ica A and ica D positive. While the (clf A, fnb A and cna) were positive in S. aureus and negative in S. epidermidis. This study confirms the presence of clf A, fnb A and can and ica A/D genes in the majorty studies S. aureus strains isolates from different sites of infection. Keywords:Staphylococcus aureus,S.epidermidis,Biofilm production,Adhesion gene

A novel biological rote of ?-L-fucose in mutans group streptococci

This study includes (50) samples were collected from patients with dental diseases (30) swabs taken from dental carries and (20) swabs from periodontal cases, these patients were of both sexes, their ages ranged from 10-65 years. There results of bacterial culture were positive in (20) patients of mutans group streptococci. Versus (30) patients revealed other types of and negative cultures.it was found that (10) isolates (50%) were identified as St. mutans, where (8) isolates (40%) identified as St. salivaris, and (2) isolates (10%) identified as St. oralis. The inhibition effect of fucose in different concentration of bacterial growth were studied, the results showed that there is a great inhibition growth on all studied bacterial isolated form oral cavity (mutans group streptococci) and the best inhibition concentration of fucose on bacterial growth found to be (80mM). Key words: Mutans group streptococci, Fucose

Antibacterial Activity of Citrullus Colocynthis against different types of bacteria

In the present study, antibacterial efficiency of citrullus colocynthesis (L.) schrad was Studied on seven types of pathogenic bacteria isolated from different sites infection, Escherichia. coli, Klebsiella pneumonia, Proteus mirabilis, Streptococcus pneumonia ,Streptococcus agalactia, Streptococcus mutans and Staphylococcus aureus. Water and ethanol extract were used for antibacterial assay. The inhibitory effects of this extracts were compared with standard antibiotics, cefotaxime. The ethanolic extract showed inhibitory activity against Escherichia. Coli, Proteus mirabilis, Staphylococcus aureus, Streptococcus agalactia. Water extract exhibited less or no activity against all types of bacteria. Results Indicated that ethanolic extract has a similar inhibitory effect with cefotaxime. Keywords: citrullus colocynthesis, ethanol extracts, cefotaxime, antibacterial activit

Effect of some factors on extracellular hemolysin filtrate from bacterial Pseudomonas aeruginosa isolated from burn infection in Hilla city

A total of (50) samples were collected from burn unit of Hilla Teaching Hospital, isolated from patient who suffering from burn cases only (40) (80%) samples were positive culture while other was negative culture (10) (20%), so, a total of (40) samples were isolated; these samples were subjected on different types of media to isolated Pseudomonas aeruginosa. Out of (40) samples, only (15) (37.5%) isolated were belonged to Pseudomonas aeruginosa. the ability of Pseudomonas aeruginosa isolated from patients with burn infection to produced capsule was studied; it was fond that all these isolated (100%) have capsules surrounding bacterial cell, and all isolated have not ability to produce sidrophores enzymes. Also it was studied the group of enzymes produced from bacteria, it was found that all these isolated have ability to produce lipase, protease, urease and bacitracin enzymes. On the other hand, the hemolysin production by Pseudomonas aeruginosa was studied and it was found that all these isolates of these bacteria were able to produce hemolysin on blood agar. Also, extracellular hemolysin filtrates of P. aeruginosa was detected on blood agar, it was found that (10) (66.6%) isolated of theses bacteria have extracellular hemolysin filtrates, it was appearance as transparent area around the well filled with hemolysin filtrates after incubation for 24 hours. The effect of H2O2 on extracellular hemolysin filtrate of P. aeruginosa was studied, and it was found that the H2O2 was decrease the activity of extracellular hemolysin filtrate on blood agar. So, the effect of mannitol and glycerol on hemolysin filtrate produce by P. aeruginosa was studied, and it was found that all isolates of hemolysin filtrate will increased activity when added mannitol and glycerol to it. Finally, the effect of phospholipase C on hemolysin filtrate produce by P. aeruginosa was studied, and it was found that all isolates of hemolysin filtrate will increase activity when added phospholipase C to it

Biological Application of New Organic Derivatives on Bacterial Growth

The growth of strains of deferent types of pathogenic bacteria isolated from different sites of infection , Escherichia coli ,klebisiella pneumonia ,staphylococcus aurous ,staphylococcus epidermis , streptococcus pneumonia, streptococcus agalactiae , streptococcus mutans, has been investigated about inhibitory effect of new synthesized complex organic compound consisting of ( Ni+2 or Co+2) . The results reveled that cobalt (II) complex which contain chelating dicarboxylate ligands has action inhibitory effect on such pathogenic bacteria while Ni ( II) Shows no effect even in highly concentration but only on the streptococcus agalactiae. Key word : Pathogenic bacteria , Diacetic acid  derivative , antibacterial activity .

Antibacterial Activity of Aluminum Potassium Sulfate and Syzygium Aromaticum Extract Against Pathogenic Microorganisms

Natural products have been used for centuries in treating human disease. The present study aimed to determine the antibacterial ability of aluminum potassium sulfate (alum) and Syzygium aromaticum (clove) extract against different types of pathogenic bacteria in scientific way. Antimicrobial activity of crude extract from two commonly used medicinally materials, alum and clove were evaluated against four microorganisms over different concentration (10, 20, 30, 40 and 50) w/v %. Minimum inhibitory concentration (MIC) and the diameter of inhibition zone were determined by in vitro bioassay using agar well diffusion method against S. aureus, S. epidermidis, E. coli, Klebsiella pneumonia. The two medicinal materials exhibited antibacterial activity and inhibition growth of gram positive and gram negative bacteria isolated from different sites of infection. The inhibition effect of these extracts compared with standard antibiotics cefotaxime. In conclusions, the results of present study revealed that the alum and clove could be used as natural antimicrobial in different ways. Keywords:Alum, clove, Syzygium aromaticum, S. aureus, S. epidermidis, E. coli, Klebsiella pneumonia, cefotaxim

Antibacterial Activity of Carvacrol against Different Types of Bacteria

In the present study, antibacterial efficiency of Carvacrol was studied on nine types of pathogenic bacteria isolated from different clinical samples, S. aureus, S. epidermidis, St. pneumonia? , E. coli, Klebsiella pneumonia, Proteus mirabilis, Pseudomonas aeroginosa,  Enterobacter spp. and Serratia spp. the inhibitory effects of this oil were compared with standard antibiotics, ciprofloxacin. The inhibition effect of Carvacrol in different concentration of bacterial growth were studied, the results showed that there is a great inhibition growth on all studied bacterial isolates except Pseudomonas aeroginosa. Keywords: Carvacrol, Antibacterial, Thymus, Vulgaris, Ciprofloxacin

Involvement of fucose in bacterial oxidative stress processes

In this research, bacteria oxidative stress processes was investigated in order to evaluate the role of L-fucose correlated with xanthine oxidase activity in the release of toxin generated by pathogenic bacteria such as Staphylococcus aureus, Klebsiella peneumonia and Escherichia coli (display as normal flora). The ratio of total fucose/ total protein obtained proved that L- fucose can act as an antioxidant factor in cell. The obtained ratio increased 4 and 8.37 times fold in Staphylococcus aureus and Klebsiella peneumonia respectively than in normal bacteria

Antibacterial Activity of 2- (2-Hydroxy phenylimino) Acetic Acid

The ability of 2-(2-hydroxy phenylimono) acetic acid [2-(2- HP-AA)]  to inhibit growth of different types of pathogenic bacteria was investigated.Different concentration were prepared from this chemical compounds( 40 mM ,50 mM and 70 mM). E.coli, staphylococcus aureus and streptococcus agalactiae nearly completely inhibited at 70 mM ,while the same concentration did not inhibit the growth of  Proteus mirabilis ,streptococcus mutans, Klebsiella pneumoniae and streptococcus pneumonia .after 24 h incubating in the present study we evaluate that 70 mM considered as the minimum inhibitory concentration. Key words: Acetic acid, Acetic acid derivatives 2-(2- HP-AA) , Antibacterial effect , Cytoplasmic P

Inhibitory effect of new ligand 2, 3, 5, 6, 0, 0, 0, 0- tetra acetic acid-L- ascorbic acid on pathogenic bacteria

The inhibitory effect of new product (2, 3, 5, 6, 0, 0, 0, 0- tetra acetic acid-L- ascorbic acid)(V-C-4Ac) on growth of different types of pathogenic bacteria was investigated. Different concentrations were prepared from (V-C-4Ac) , (1%, 3%,  4% and 5%) nearly completely inhibited the growth of Streptococcus pneumoniae and Streptococcus agalactiae after 24 hrs incubation, and the same range of concentrations did not inhibit the growth of Streptococcus mutans, Klebsiella pneumonia and Proteus mirabilis. Also, this study shows  low effect of new ligand in the growth of Staphylococcus aureus and E. coli in high concentration (5%) after 2 hrs incubation. Keywords: L- ascorbic acid, New ligand synthesis (V-C,4Ac) , Antibacterial effect