5,711 research outputs found
Defective Gut Function in \u3cem\u3eDrop-Dead\u3c/em\u3e Mutant \u3cem\u3eDrosophila\u3c/em\u3e
Mutation of the gene drop-dead (drd ) causes adult Drosophila to die within 2 weeks of eclosion and is associated with reduced rates of defecation and increased volumes of crop contents. In the current study, we demonstrate that flies carrying the strong allele drdlwf display a reduction in the transfer of ingested food from the crop to the midgut, as measured both as a change in the steady-state distribution of food within the gut and also in the rates of crop emptying and midgut filling following a single meal. Mutant flies have abnormal triglyceride (TG) and glycogen stores over the first 4 days post-eclosion, consistent with their inability to move food into the midgut for digestion and nutrient absorption. However, the lifespan of mutants was dependent upon food presence and quality, suggesting that at least some individual flies were able to digest some food. Finally, spontaneous motility of the crop was abnormal in drdlwf flies, with the crops of mutant flies contracting significantly more rapidly than those of heterozygous controls. We therefore hypothesize that mutation of drd causes a structural or regulatory defect that inhibits the entry of food into the midgut
Solute channels of the outer membrane: from bacteria to chloroplasts
Chloroplasts, unique organelles of plants, originated from endosymbiosis of an ancestor of today's cyanobacteria with a mitochondria-containing host cell. It is assumed that the outer envelope membrane, which delimits the chloroplast from the surrounding cytosol, was thus inherited from its Gram-negative bacterial ancestor. This plastid-specific membrane is thus equipped with elements of prokaryotic and eukaryotic origin. In particular, the membrane-intrinsic outer envelope proteins (OEPs) form solute channels with properties reminiscent of porins and channels in the bacterial outer membrane. OEP channels are characterised by distinct specificities for metabolites and a quite peculiar expression pattern in specialised plant organs and plastids, thus disproving the assumption that the outer envelope is a non-specific molecular sieve. The same is true for the outer membrane of Gram-negative bacteria, which functions as a permeability barrier in addition to the cytoplasmic membrane, and embeds different classes of channel pores. The channels of these prokaryotic prototype proteins, ranging from unspecific porins to specific channels to ligand-gated receptors, are exclusively built of P-barrels. Although most of the OEP channels are formed by P-strands as well, phylogeny based on sequence homology alone is not feasible. Thus, the comparison of structural and functional properties of chloroplast outer envelope and bacterial outer membrane channels is required to pinpoint the ancestral OEP `portrait gallery'
Does Medicaid Make a Difference? Findings from the Commonwealth Fund Biennial Health Insurance Survey, 2014
As millions of Americans gain Medicaid coverage under the Affordable Care Act, attention has focused on the access to care, quality of care, and financial protection that coverage provides. This analysis uses the Commonwealth Fund Biennial Health Insurance Survey, 2014, to explore these questions by comparing the experiences of working-age adults with private insurance who were insured all year, Medicaid beneficiaries with a full year of coverage, and those who were uninsured for some time during the year. The survey findings suggest that Medicaid coverage provides access to care that in most aspects is comparable to private insurance. Adults with Medicaid coverage reported better care experiences on most measures than those who had been uninsured during the year. Medicaid beneficiaries also seem better protected from the cost of illness than do uninsured adults, as well as those with private coverage
The Role of a Hot Gas Environment on the Evolution of Galaxies
Most spiral galaxies are found in galaxy groups with low velocity
dispersions; most E/S0 galaxies are found in galaxy groups with relatively high
velocity dispersions. The mass of the hot gas we can observe in the E/S0 groups
via their thermal X-ray emission is, on average, as much as the baryonic mass
of the galaxies in these groups. By comparison, galaxy clusters have as much or
more hot gas than stellar mass. Hot gas in S-rich groups, however, is of low
enough temperature for its X-ray emission to suffer heavy absorption due to
Galactic HI and related observational effects, and hence is hard to detect. We
postulate that such lower temperature hot gas does exist in low velocity
dispersion, S-rich groups, and explore the consequences of this assumption. For
a wide range of metallicity and density, hot gas in S-rich groups can cool in
far less than a Hubble time. If such gas exists and can cool, especially when
interacting with HI in existing galaxies, then it can help link together a
number of disparate observations, both Galactic and extragalactic, that are
otherwise difficult to understand.Comment: 16 pages with one figure. ApJ Letters, in pres
A Causal Order for Spacetimes with Lorentzian Metrics: Proof of Compactness of the Space of Causal Curves
We recast the tools of ``global causal analysis'' in accord with an approach
to the subject animated by two distinctive features: a thoroughgoing reliance
on order-theoretic concepts, and a utilization of the Vietoris topology for the
space of closed subsets of a compact set. We are led to work with a new causal
relation which we call , and in terms of it we formulate extended
definitions of concepts like causal curve and global hyperbolicity. In
particular we prove that, in a spacetime \M which is free of causal cycles,
one may define a causal curve simply as a compact connected subset of \M
which is linearly ordered by . Our definitions all make sense for
arbitrary metrics (and even for certain metrics which fail to be
invertible in places). Using this feature, we prove for a general metric,
the familiar theorem that the space of causal curves between any two compact
subsets of a globally hyperbolic spacetime is compact. We feel that our
approach, in addition to yielding a more general theorem, simplifies and
clarifies the reasoning involved. Our results have application in a recent
positive energy theorem, and may also prove useful in the study of topology
change. We have tried to make our treatment self-contained by including proofs
of all the facts we use which are not widely available in reference works on
topology and differential geometry.Comment: Two small revisions to accomodate errors brought to our attention by
R.S. Garcia. No change to chief results. 33 page
Inverse Compton Scattering as the Source of Diffuse EUV Emission in the Coma Cluster of Galaxies
We have examined the hypothesis that the majority of the diffuse EUV flux in
the Coma cluster is due to inverse Compton scattering of low energy cosmic ray
electrons (0.16 < epsilon < 0.31 GeV) against the 3K black-body background. We
present data on the two-dimensional spatial distribution of the EUV flux and
show that these data provide strong support for a non-thermal origin for the
EUV flux. However, we show that this emission cannot be produced by an
extrapolation to lower energies of the observed synchrotron radio emitting
electrons and an additional component of low energy cosmic ray electrons is
required.Comment: 11 pages, 5 figure
Forman's Ricci curvature - From networks to hypernetworks
Networks and their higher order generalizations, such as hypernetworks or
multiplex networks are ever more popular models in the applied sciences.
However, methods developed for the study of their structural properties go
little beyond the common name and the heavy reliance of combinatorial tools. We
show that, in fact, a geometric unifying approach is possible, by viewing them
as polyhedral complexes endowed with a simple, yet, the powerful notion of
curvature - the Forman Ricci curvature. We systematically explore some aspects
related to the modeling of weighted and directed hypernetworks and present
expressive and natural choices involved in their definitions. A benefit of this
approach is a simple method of structure-preserving embedding of hypernetworks
in Euclidean N-space. Furthermore, we introduce a simple and efficient manner
of computing the well established Ollivier-Ricci curvature of a hypernetwork.Comment: to appear: Complex Networks '18 (oral presentation
Americans' Experiences in the Health Insurance Marketplaces: Results from The First Three Months
Conducted December 11–29, 2013, The Commonwealth Fund's second Affordable Care Act Tracking Survey interviewed a nationally representative sample of adults who are potentially eligible for the health reform law's new insurance options, whether private plans or Medicaid. Results show that by the end of December, 24 percent of potentially eligible adults had visited a marketplace to find a plan. The first survey, conducted in October, had found that 17 percent of people potentially eligible for coverage had visited the marketplaces during the first month of open enrollment. By the end of December, 41 percent of visitors were ages 19 to 34, and 77 percent reported being in good health. People's ability to compare benefits and premiums improved between October and December, but many reported challenges in plan selection. A majority of respondents say they are determined to gain coverage by the end of this year's open enrollment period
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