3 research outputs found
Recommended from our members
Experiments with Monopoles, Rings and Knots in Spinor Bose-Einstein Condensates
Topological excitations are ubiquitous in nature, their charge being a naturally-quantized, conserved quantity that can exhibit particle-like behavior. Spinor Bose-Einstein condensates (BECs) are an exceptionally versatile system for the study and exploration of topological excitations. Between the spin-1 and spin-2 87Rb condensates there are seven possible broken-symmetry magnetic phases, with each one hosting unique opportunities for topological defects. We have created and observed several novel topological excitations in a spinor 87Rb BEC. In this dissertation I present and discuss three principal experimental findings: (1) The discovery of an Alice ring, or a half-quantum vortex ring, emerging from a monopole singularity in a nematic spin-1 BEC; (2) The first realization of a \u27t Hooft-Polyakov-type monopole in a nematic spin-2 BEC; and (3) The first realization of a knot in a nematic spin-2 BEC. This dissertation concludes with possible avenues for future research
The Study of Viral RNA Diversity in Bird Samples Using De Novo Designed Multiplex Genus-Specific Primer Panels
Advances in the next generation sequencing (NGS) technologies have significantly increased our ability to detect new viral pathogens and systematically determine the spectrum of viruses prevalent in various biological samples. In addition, this approach has also helped in establishing the associations of viromes with many diseases. However, unlike the metagenomic studies using 16S rRNA for the detection of bacteria, it is impossible to create universal oligonucleotides to target all known and novel viruses, owing to their genomic diversity and variability. On the other hand, sequencing the entire genome is still expensive and has relatively low sensitivity for such applications. The existing approaches for the design of oligonucleotides for targeted enrichment are usually involved in the development of primers for the PCR-based detection of particular viral species or genera, but not for families or higher taxonomic orders. In this study, we have developed a computational pipeline for designing the oligonucleotides capable of covering a significant number of known viruses within various taxonomic orders, as well as their novel variants. We have subsequently designed a genus-specific oligonucleotide panel for targeted enrichment of viral nucleic acids in biological material and demonstrated the possibility of its application for virus detection in bird samples. We have tested our panel using a number of collected samples and have observed superior efficiency in the detection and identification of viral pathogens. Since a reliable, bioinformatics-based analytical method for the rapid identification of the sequences was crucial, an NGS-based data analysis module was developed in this study, and its functionality in the detection of novel viruses and analysis of virome diversity was demonstrated