Editorial: Roots—the hidden provider

No abstract available

Exploring emergent properties in cellular homeostasis using OnGuard to model K+ and other ion transport in guard cells

It is widely recognized that the nature and characteristics of transport across eukaryotic membranes are so complex as to defy intuitive understanding. In these circumstances, quantitative mathematical modeling is an essential tool, both to integrate detailed knowledge of individual transporters and to extract the properties emergent from their interactions. As the first, fully integrated and quantitative modeling environment for the study of ion transport dynamics in a plant cell, OnGuard offers a unique tool for exploring homeostatic properties emerging from the interactions of ion transport, both at the plasma membrane and tonoplast in the guard cell. OnGuard has already yielded detail sufficient to guide phenotypic and mutational studies, and it represents a key step toward ‘reverse engineering’ of stomatal guard cell physiology, based on rational design and testing in simulation, to improve water use efficiency and carbon assimilation. Its construction from the HoTSig libraries enables translation of the software to other cell types, including growing root hairs and pollen. The problems inherent to transport are nonetheless challenging, and are compounded for those unfamiliar with conceptual ‘mindset’ of the modeler. Here we set out guidelines for the use of OnGuard and outline a standardized approach that will enable users to advance quickly to its application both in the classroom and laboratory. We also highlight the uncanny and emergent property of OnGuard models to reproduce the ‘communication’ evident between the plasma membrane and tonoplast of the guard cell

Stomatal clustering in Begonia associates with the kinetics of leaf gaseous exchange and influences water use efficiency

Stomata are microscopic pores formed by specialized cells in the leaf epidermis and permit gaseous exchange between the interior of the leaf and the atmosphere. Stomata in most plants are separated by at least one epidermal pavement cell and, individually, overlay a single substomatal cavity within the leaf. This spacing is thought to enhance stomatal function. Yet, there are several genera naturally exhibiting stomata in clusters and therefore deviating from the one-cell spacing rule with multiple stomata overlaying a single substomatal cavity. We made use of two Begonia species to investigate whether clustering of stomata alters guard cell dynamics and gas exchange under different light and dark treatments. Begonia plebeja, which forms stomatal clusters, exhibited enhanced kinetics of stomatal conductance and CO2 assimilation upon light stimuli that in turn were translated into greater water use efficiency. Our findings emphasize the importance of spacing in stomatal clusters for gaseous exchange and plant performance under environmentally limited conditions

Anion channel sensitivity to cytosolic organic acids implicates a central role for oxaloacetate in integrating ion flux with metabolism in stomatal guard cells

Stomatal guard cells play a key role in gas exchange for photosynthesis and in minimizing transpirational water loss from plants by opening and closing the stomatal pore. The bulk of the osmotic content driving stomatal movements depends on ionic fluxes across both the plasma membrane and tonoplast, the metabolism of organic acids, primarily Mal (Imitate), and its accumulation and loss. Anion channels at the plasma membrane are thought to comprise a major pathway for Mal efflux during stomatal closure, implicating their key role in linking solute flux with metabolism. Nonetheless, little is known of the regulation of anion channel current (I(Cl)) by cytosolic Mal or its immediate metabolite OAA (oxaloacetate). In the present study, we have examined the impact of Mal, OAA and of the monocarboxylic acid anion acetate in guard cells of Vicia faba L. and report that all three organic acids affect I(Cl), but with markedly different characteristics and sidedness to their activities. Most prominent was a suppression of I(Cl) by OAA within the physiological range of concentrations found in vivo. These findings indicate a capacity for OAA to co-ordinate organic acid metabolism with I(Cl), through the direct effect of organic acid pool size. The findings of the present study also add perspective to in vivo recordings using acetate-based electrolytes

Energy Efficient Engine exhaust mixer model technology report addendum; phase 3 test program

The Phase 3 exhaust mixer test program was conducted to explore the trends established during previous Phases 1 and 2. Combinations of mixer design parameters were tested. Phase 3 testing showed that the best performance achievable within tailpipe length and diameter constraints is 2.55 percent better than an optimized separate flow base line. A reduced penetration design achieved about the same overall performance level at a substantially lower level of excess pressure loss but with a small reduction in mixing. To improve reliability of the data, the hot and cold flow thrust coefficient analysis used in Phases 1 and 2 was augmented by calculating percent mixing from traverse data. Relative change in percent mixing between configurations was determined from thrust and flow coefficient increments. The calculation procedure developed was found to be a useful tool in assessing mixer performance. Detailed flow field data were obtained to facilitate calibration of computer codes

A Single Atom as a Mirror of an Optical Cavity

By tightly focussing a laser field onto a single cold ion trapped in front of a far-distant dielectric mirror, we could observe a quantum electrodynamic effect whereby the ion behaves as the optical mirror of a Fabry-P\'erot cavity. We show that the amplitude of the laser field is significantly altered due to a modification of the electromagnetic mode structure around the atom in a novel regime in which the laser intensity is already changed by the atom alone. e propose a direct application of this system as a quantum memory for single photons.Comment: 7 pages, 3 figures, to appear in Physical Review Letter

Effective generation of cat and kitten states

We present an effective method of coherent state superposition (cat state) generation using single trapped ion in a Paul trap. The method is experimentally feasible for coherent states with amplitude $\alpha \le 2$ using available technology. It works both in and beyond the Lamb-Dicke regime.Comment: 11 pages, 8 figure