Assessing agro-hydrological models to schedule irrigation for crops of Mediterranean enviroment

Despite in Mediterranean environment water resources for irrigation are limited, water management for agriculture is often practiced ignoring principles of environmental sustainability. Objective of the paper is to asses the possibility of using agro-hydrological models for irrigation scheduling, in order to optimize the water use efficiency. The results of a comparison between the numerical SWAP model and the functional model proposed by FAO to estimate water requirements in two typical arboreal Mediterranean Crops (grapevine and olive) are showed. In the initial phase of the research, involving both irrigation seasons 2005 and 2006, after a preliminary analysis of soil hydraulic and biophysical plant parameters, two intensive field measurements campaigns were carried out to measure the soil water content at different depths, to proceed to the validation of both the models. Validation of the model was carried out by means of the comparison between measured and predicted soil water content. Finally different irrigation scheduling options were examined, in order to compare the scheduled irrigation times with those planned by the farmers. The results of investigations evidenced that FAO model simulates reliably the values of average water content of the soil profile, even if a certain overestimation of evapotranspiration fluxes can be observed with the FAO 56 model compared with SWAP. Consequently, the FAO model anticipates the starting date for irrigation obtained with SWAP, but, in terms of seasonal water requirements, the estimates determined by the two modes did not result significantly different

Uso di modelli agroidrologici per la gestione dell’irrigazione di colture arboree mediterranee

Nel presente lavoro viene presentato un confronto tra due modelli di bilancio idrologico: il modello proposto dalla FAO e il modello SWAP, basato sulla soluzione dell’equazione di Richards. Il confronto ha riguardato i valori delle componenti del bilancio idrologico ed i contenuti idrici del suolo relativamente alle due stagioni irrigue 2005 e 2006 su colture di Vite ed Olivo. È stata inoltre valutata la performance dei due modelli sulla programmazione dell’irrigazione impostando i parametri di scheduling ordinari della zona

Estimation of actual evapotranspiration of Mediterranean perennial crops by means of remote-sensing based surface energy balance models.

Actual evapotranspiration from typical Mediterranean crops has been assessed in a Sicilian study area by using surface energy balance (SEB) and soil-water balance models. Both modelling approaches use remotely sensed data to estimate evapotranspiration fluxes in a spatially distributed way. The first approach exploits visible (VIS), near infrared (NIR) and thermal (TIR) observations to solve the surface energy balance equation whereas the soil-water balance model uses only VIS-NIR data to detect the spatial variability of crop parameters. Considering that the study area is characterized by typical spatially sparse Mediterranean vegetation, i.e. olive, citrus and vineyards, alternating bare soil and canopy, we focused the attention on the main conceptual differences between one-source and two-sources energy balance models. Two different models have been tested: the widely used one-source SEBAL model, where soil and vegetation are considered as the sole source (mostly appropriate in the case of uniform vegetation coverage) and the two sources TSEB model, where soil and vegetation components of the surface energy balance are treated separately. Actual evapotranspiration estimates by means of the two surface energy balance models have been compared vs. the outputs of the agro-hydrological SWAP model, which was applied in a spatially distributed way to simulate one-dimensional water flow in the soil-plant-atmosphere continuum. Remote sensing data in the VIS and NIR spectral ranges have been used to infer spatially distributed vegetation parameters needed to set up the upper boundary condition of SWAP. Actual evapotranspiration values obtained from the application of the soil water balance model SWAP have been considered as the reference to be used for energy balance models accuracy assessment. Airborne hyperspectral data acquired during a NERC (Natural Environment Research Council, UK) campaign in 2005 have been used. The results of this investigation seem to prove a slightly better agreement between SWAP and TSEB for some fields of the study area. Further investigations are programmed in order to confirm these indications

Estimation of actual evapotranspiration of Mediterranean perennial crops by means of remote-sensing based surface energy balance models

Actual evapotranspiration from typical Mediterranean crops has been assessed in a Sicilian study area by using surface energy balance (SEB) and soil-water balance models. Both modelling approaches use remotely sensed data to estimate evapotranspiration fluxes in a spatially distributed way. The first approach exploits visible (VIS), near-infrared (NIR) and thermal (TIR) observations to solve the surface energy balance equation whereas the soil-water balance model uses only VIS-NIR data to detect the spatial variability of crop parameters. Considering that the study area is characterized by typical spatially sparse Mediterranean vegetation, i.e. olive, citrus and vineyards, alternating bare soil and canopy, we focused the attention on the main conceptual differences between one-source and two-sources energy balance models. Two different models have been tested: the widely used one-source SEBAL model, where soil and vegetation are considered as the sole source (mostly appropriate in the case of uniform vegetation coverage) and the two-sources TSEB model, where soil and vegetation components of the surface energy balance are treated separately. Actual evapotranspiration estimates by means of the two surface energy balance models have been compared vs. the outputs of the agro-hydrological SWAP model, which was applied in a spatially distributed way to simulate one-dimensional water flow in the soil-plant-atmosphere continuum. Remote sensing data in the VIS and NIR spectral ranges have been used to infer spatially distributed vegetation parameters needed to set up the upper boundary condition of SWAP. Actual evapotranspiration values obtained from the application of the soil water balance model SWAP have been considered as the reference to be used for energy balance models accuracy assessment. <br><br> Airborne hyperspectral data acquired during a NERC (Natural Environment Research Council, UK) campaign in 2005 have been used. The results of this investigation seem to prove a slightly better agreement between SWAP and TSEB for some fields of the study area. Further investigations are programmed in order to confirm these indications

Immune response to tick-borne hemoparasites: Host adaptive immune response mechanisms as potential targets for therapies and vaccines

Tick-transmitted pathogens cause infectious diseases in both humans and animals. Different types of adaptive immune mechanisms could be induced in hosts by these microorganisms, triggered either directly by pathogen antigens or indirectly through soluble factors, such as cytokines and/or chemokines, secreted by host cells as response. Adaptive immunity effectors, such as antibody secretion and cytotoxic and/or T helper cell responses, are mainly involved in the late and long-lasting protective immune response. Proteins and/or epitopes derived from pathogens and tick vectors have been isolated and characterized for the immune response induced in different hosts. This review was focused on the interactions between tick-borne pathogenic hemoparasites and different host effector mechanisms of T-and/or B cell-mediated adaptive immunity, describing the efforts to define immunodominant proteins or epitopes for vaccine development and/or immunotherapeutic purposes. A better understanding of these mechanisms of host immunity could lead to the assessment of possible new immunotherapies for these pathogens as well as to the prediction of possible new candidate vaccine antigens

Recent Advances on the Innate Immune Response to Coxiella burnetii.

Coxiella burnetii is an obligate intracellular Gram-negative bacterium and the causative agent of a worldwide zoonosis known as Q fever. The pathogen invades monocytes and macrophages, replicating within acidic phagolysosomes and evading host defenses through different immune evasion strategies that are mainly associated with the structure of its lipopolysaccharide. The main transmission routes are aerosols and ingestion of fomites from infected animals. The innate immune system provides the first host defense against the microorganism, and it is crucial to direct the infection towards a self-limiting respiratory disease or the chronic form. This review reports the advances in understanding the mechanisms of innate immunity acting during C. burnetii infection and the strategies that pathogen put in place to infect the host cells and to modify the expression of specific host cell genes in order to subvert cellular processes. The mechanisms through which different cell types with different genetic backgrounds are differently susceptible to C. burnetii intracellular growth are discussed. The subsets of cytokines induced following C. burnetii infection as well as the pathogen influence on an inflammasome-mediated response are also described. Finally, we discuss the use of animal experimental systems for studying the innate immune response against C. burnetii and discovering novel methods for prevention and treatment of disease in humans and livestock

Detection of natural killer T cells in mice infected with Rickettsia conorii

Little information is available regarding the role of natural killer T (NKT) cells during the early stage of Rickettsia conorii infection. Herein, C3H/HeN mice were infected with the Malish 7 strain of R. conorii. Splenocytes from these mice were analysed in the early stage of the infection by flow cytometry and compared with uninfected controls. Our results showed an increase in NKT cells in infected mice. Additionally, NKT interleukin (IL)-17(+) cells increased three days after infection, together with a concurrent decrease in the relative amount of NKT interferon (IFN)-\u3b3(+) cells. We also confirmed a higher amount of NK IFN-\u3b3(+) cells in infected mice. Taken together, our data showed that NKT cells producing Il-17 increased during the early stage of rickettsial infection. These results suggest a connection between IL-17(+) NKT cells and vasculitis, which is the main clinical symptom of rickettsiosi

Potential roles of extracellular vesicles in brain cell-to-cell communication

Potential roles of extracellular vesicles in brain cell-to-cell communication Extracellular vesicles (EVs) are released into thè extracellular space from both cancer and normal brain cells, and are probably able to modify thè phenotypic properties of receiving cells1. EVs released from astrocytes and neurons contain FGF2 and VEGF2'3 and induce a 'blood-brain barrier' (BBB) phenotype in cultured brain capillary endothelial cells (BCECs, unpublished results), On thè other hand, EVs from G26/24 oligodendroglioma induce apoptosis in neurons and astrocytes4-5. These effects are probably due to Fas Ligand and TRAIL, present in G26/24 vesicles4-5. Moreover, G26/24 EVs contain extracellular matrix remodeling proteases (such as ADAMTS)6, H1.0 histone protein, and H1.0 mRNA7. In particular, we previously hypothesized that G26/24 cells, and tumor cells in generai, can escape differentiation cues, and continue to proliferate by eliminating proteins, such as thè H1° linker histone (and its mRNA)7, which could otherwise block proliferation. To study vesicle release in a System that can better resemble in vivo conditions, astrocytes and BCECs were cultured on poly-L-lactic acid (PLLA) scaffolds and tested for their ability to grow and survive on this three-dimensional structures. We analyzed in parallel thè celi growth in 2D and 3D culture systems and observed thè differences in celi morphology by fluorescence analysis: threedimensional scaffolds have thè ability to guide celi growth, provide support, encourage celi adhesion and proliferation. Astrocytes8 and BCECs (unpublished results) adapted well to these porous matrices, not only remaining on thè surface, but also penetrating inside thè scaffolds. EVs released by astrocytes in these scaffolds are probably exosomes, as suggested by transmission electron microscopy pictures, and by thè presence of intracellular structures resembling multivesicular bodies. This 3D celi culture System could be further enriched to host different brain celi types, in order to set, for example, an in vitro model of BBB, that may be useful for drug delivery studies, and for thè formulation of new therapeutic strategies for thè treatment of neurological diseases. References [1] Schiera, G., Di Liegro, C.M., Di Liegro I. Int J Mol Sci. 2017, 18(12). pii: E2774. [2] Schiera, G., Proia, P., Alberti, C., Mineo, M., Savettieri, G., Di Liegro, I., 2007. J Celi Mol Med. 2007, 111(6), 1384-94. [3] Proia, P., Schiera, G., Mineo, M., Ingrassia, A.M. Santoro, G., Savettieri, G., Di Liegro, I. Int J Mol Med. 2008, 21(1), 63-7. [4] D'Agostino, S., Salamene, M., Di Liegro, I., Vittorelli, ML, Int J Oncol. 2006, 29(5), 1075-85. [5] Lo Cicero, A., Schiera, G., Proia, P., Saladino, P., Savettieri, G., Di Liegro, C.M., Di Liegro, I. Int J Oncol. 2011,39(6): 1353-7. [6] Lo Cicero, A., Majkowska, I., Nagase, H., Di Liegro, I., Troeberg, L., Matrix Biol. 2012, 31(4), 229-33. [7] Schiera, G., Di Liegro, C.M., Saladino, P., Pitti, R., Savettieri, G., Proia, P., Di Liegro, I. Int J Oncol. 2013, 43(6), 1771-6. [8] Carfì Pavia, F., Di Bella, M.A., Brucato, V., Blanda, V., Zummo, F., Vitrano, I., Di Liegro, C.M., Ghersi, G., Di Liegro, I., Schiera, G. Mol Med Rep. 2019 [Epub ahead of print]. [9] Di Bella MA, Zummo F., Carfì Pavia F., Brucato V., Di Liegro I., Schiera G. 2017, In: Microscopy and Imaging Science: practical approaches to applied research and education, pp 260-264. Ed: A. Méndez-Vilas Publisher, Formatex Research Center (Spain), ISBN-13, 978-84-942134-9-6

Biotic and abiotic factors shape the microbiota of wild-caught populations of the arbovirus vector Culicoides imicola

Biting midges of the genus Culicoides are known vectors of arboviruses affecting human and animal health. However, little is known about Culicoides imicola microbiota and its influence on this insect’s biology. In this study, the impact of biotic and abiotic factors on C. imicola microbiota was characterized using shotgun-metagenomic sequencing of whole-body DNA samples. Wild-caught C. imicola adult nulliparous females were sampled in two locations from Sicily, Italy. The climatic variables of temperature and soil moisture from both localities were recorded together with potential host bloodmeal sources. Shared core microbiome among C. imicola populations included Pseudomonas, Escherichia, Halomonas, Candidatus Zinderia, Propionibacterium, and Schizosaccharomyces. Specific and unique taxa were also found in C. imicola from each location, highlighting similarities and differences in microbiome composition between the two populations. DNA and protein identification showed differences in host preferences between the two populations, with Homo sapiens and Canis lupus familiaris L. being the preferred bloodmeal source in both locations. A principal component analysis showed that the combined effect of host preferences (H. sapiens) and local soil moisture factors shape the microbiome composition of wild-caught populations of C. imicola. These results contribute to characterizing the role of the microbiome in insect adaptation and its utility in predicting geographic expansion of Culicoides species with potential implications for the control of vector-borne diseases