Measurement of correlation between transmission and scattering during wound healing in hen corneas

Producción CientíficaThe aim of this work is to provide experimental data for corneal transparency and scattering to help create a more complete model of corneal transparency. The scattered light in 96 healing hen corneas was measured for three wavelengths by a scatterometer constructed in the Optics Laboratory (The University of Valladolid, Spain). With the help of mirrors and beamsplitters, the light from the three lasers is directed toward the cell containing the sample to be measured. The measured scattered light varies between six orders of magnitude. Corneal transmissivity, mean cosine of a scattering angle, and angular distribution of scattered light were all computed. The total transmitted light remained practically constant over a wide range of light values transmitted in a forward direction (direct transmissivity). The value of the mean cosine of the scattering direction is very close to the unit (g40:98), even in corneas with high opacities. The behavior of g indicates that even damaged corneas evidence extremely small scattering, compared to other biological tissues. The transmission reduction of each cornea is related to an increase in scattered light. In all cases, scattered light is concentrated at very small angles. This behavior is acceptable in corneas that are healthy or which evidence small lesions, but remains in corneas that are severely injured

The use of genetically engineered mice and imaging systems for understanding the pathophysiology of the cornea

Esta tesis doctoral aborda el estudio de la fisiopatología de la córnea desde el punto de vista de la cicatrización, la inflamación y el estado normal tanto de la población residente de células inmunitarias como de el sistema nervioso periférico que la inerva. Por otro lado, en esta tesis se muestra una evolución tecnológica, desde el capítulo 1 hasta el capítulo 7, tanto en los sistemas de imagen como en los ratones utilizados. De esta manera se muestra la córnea como un órgano extremadamente complejo, en el que varios sistemas interaccionan entre si para mantener sus propiedades fundamentales. Entendiendo la córnea desde varios enfoques y con la posibilidad de implementar nuevos sistemas de imagen “in vivo” se da un avance considerable en el estudio de la patologías a tiempo real

αVβ6 Integrin Promotes Corneal Wound Healing

The lack of αVβ6 impairs basement membrane and mature hemidesmosome reformation after a superficial keratectomy wound in mouse corneas