Climate Benefits Tenure Costs: The Economic Case for Securing Indigenous Land Rights in the Amazon

A new report offers evidence that the modest investments needed to secure land rights for indigenous communities will generate billions in returns—economically, socially and environmentally—for local communities and the world's changing climate. The report, Climate Benefits, Tenure Costs: The Economic Case for Securing Indigenous Land Rights, quantifies for the first time the economic value of securing land rights for the communities who live in and protect forests, with a focus on Colombia, Brazil, and Bolivia

Climate Benefits Tenure Costs: The Economic Case for Securing Indigenous Land Rights in the Amazon, Executive Summary

A new report offers evidence that the modest investments needed to secure land rights for indigenous communities will generate billions in returns—economically, socially and environmentally—for local communities and the world's changing climate. The report, Climate Benefits, Tenure Costs: The Economic Case for Securing Indigenous Land Rights, quantifies for the first time the economic value of securing land rights for the communities who live in and protect forests, with a focus on Colombia, Brazil, and Bolivia

Geological interpretation of current subsidence and uplift in the London area, UK, as shown by high precision satellite-based surveying

Long term planning for flood risk management in coastal areas requires timely and reliable information on changes in land and sea levels. A high resolution map of current changes in land levels in the London and Thames estuary area has been generated by satellite-based persistent scatterer interferometry (PSI), aligned to absolute gravity (AG) and global positioning system (GPS) measurements. This map has been qualitatively validated by geological interpretation, which demonstrates a variety of controlling influences on the rates of land level change, ranging from near-surface to deep-seated mechanisms and from less than a decade to more than 100,000 years’ duration. During the period 1997–2005, most of the region around the Thames estuary subsided between 0.9 and 1.5 mm a−1 on average, with subsidence of thick Holocene deposits being as fast as 2.1 mm a−1. By contrast, parts of west and north London on the Midlands Microcraton subsided by less than 0.7 mm a−1, and in places appear to have risen by about 0.3 mm a−1. These rates of subsidence are close to values determined previously by studies of Quaternary sequences, but the combined GPS, AG and PSI land level change data demonstrate a new level of local geological control that was not previously resolvabl

A malaria parasite subtilisin propeptide-like protein is a potent inhibitor of the egress protease SUB1.

Subtilisin-like serine peptidases (subtilases) play important roles in the life cycle of many organisms, including the protozoan parasites that are the causative agent of malaria, Plasmodium spp. As with other peptidases, subtilase proteolytic activity has to be tightly regulated in order to prevent potentially deleterious uncontrolled protein degradation. Maturation of most subtilases requires the presence of an N-terminal propeptide that facilitates folding of the catalytic domain. Following its proteolytic cleavage, the propeptide acts as a transient, tightly bound inhibitor until its eventual complete removal to generate active protease. Here we report the identification of a stand-alone malaria parasite propeptide-like protein, called SUB1-ProM, encoded by a conserved gene that lies in a highly syntenic locus adjacent to three of the four subtilisin-like genes in the Plasmodium genome. Template-based modelling and ab initio structure prediction showed that the SUB1-ProM core structure is most similar to the X-ray crystal structure of the propeptide of SUB1, an essential parasite subtilase that is discharged into the parasitophorous vacuole (PV) to trigger parasite release (egress) from infected host cells. Recombinant Plasmodium falciparum SUB1-ProM was found to be a fast-binding, potent inhibitor of P. falciparum SUB1, but not of the only other essential blood-stage parasite subtilase, SUB2, or of other proteases examined. Mass-spectrometry and immunofluorescence showed that SUB1-ProM is expressed in the PV of blood stage P. falciparum, where it may act as an endogenous inhibitor to regulate SUB1 activity in the parasite

Temporal and spatial variation in distribution of fish environmental DNA in England’s largest lake

Environmental DNA offers great potential as a biodiversity monitoring tool. Previous work has demonstrated that eDNA metabarcoding provides reliable information for lake fish monitoring, but important questions remain about temporal and spatial repeatability, which is critical for understanding the ecology of eDNA and developing effective sampling strategies. Here, we carried out comprehensive spatial sampling of England's largest lake, Windermere, during summer and winter to 1) examine repeatability of the method, 2) compare eDNA results with contemporary gill-net survey data, 3) test the hypothesis of greater spatial structure of eDNA in summer compared to winter due to differences in water mixing between seasons, and 4) compare the effectiveness of shore and offshore sampling for species detection. We find broad consistency between results from three sampling events in terms of species detection and abundance, with eDNA detecting more species than established methods and being significantly correlated to rank abundance determined by long-term data. As predicted, spatial structure was much greater in the summer, reflecting less mixing of eDNA than in the winter. For example Arctic charr, a deep-water species, was only detected in deep, mid-lake samples in the summer, while littoral or benthic species such as minnow and stickleback were more frequently detected in shore samples. By contrast in winter, the eDNA of these species was more uniformly distributed. This has important implications for design of sampling campaigns, for example, deep-water species could be missed and littoral/benthic species over-represented by focusing exclusively on shoreline samples collected in the summer

Evaluation of the i3 Scale-Up of Reading Recovery | Year Two Report, 2012-13

Reading Recovery is a short-term early intervention designed to help the lowest-achieving readers in first grade reach average levels of classroom performance in literacy. Students identified to receive Reading Recovery meet individually with a specially trained Reading Recovery teacher every school day for 30-minute lessons over a period of 12 to 20 weeks. The purpose of these lessons is to support rapid acceleration of each child’s literacy learning. In 2010, The Ohio State University received a Scaling Up What Works grant from the U.S. Department of Education Investing in Innovation (i3) Fund to expand the use of Reading Recovery across the country. The award was intended to fund the training of 3,675 new Reading Recovery teachers in U.S. schools, thereby expanding service to an additional 88,200 students. The Consortium for Policy Research in Education (CPRE) was contracted to conduct an independent evaluation of the i3 scale-up of Reading Recovery over the course of five years. The evaluation includes parallel rigorous experimental and quasi-experimental designs for estimating program impacts, coupled with a large-scale mixed-methods study of program implementation. This report presents the findings of the second year of the evaluation. The primary goals of this evaluation are: a) to provide experimental evidence of the impacts of Reading Recovery on student learning under this scale-up effort ; b) to assess the success of the scale-up in meeting the i3 grant’s expansion goals; and c) to document the implementation of the scale-up and fidelity to program standards. This document is the second in a series of three reports based on our external evaluation of the Reading Recovery i3 Scale-Up. This report presents results from the impact and implementation studies conducted over the 2012-2013 school year—the third year of the scale-up effort and the second full year of the evaluation. In order to estimate the impacts of the program, a sample of first graders who had been selected to receive Reading Recovery were randomly assigned to a treatment group that received Reading Recovery immediately, or to a control group that did not receive Reading Recovery until the treatment group had exited the intervention. The reading achievement of students in this sample was assessed using a standardized assessment of reading achievement—the Iowa Tests of Basic Skills (ITBS). The data for the implementation study include extensive interviews and surveys with Reading Recovery teachers, teacher leaders, site coordinators, University Training Center directors, members of the i3 project leadership team at The Ohio State University, and principals and first-grade teachers in schools involved in the scale-up. Case studies were also conducted in nine i3 scale-up schools to observe how Reading Recovery operates in different contexts

Parasitophorous vacuole poration precedes its rupture and rapid host erythrocyte cytoskeleton collapse in Plasmodium falciparum egress.

In the asexual blood stages of malarial infection, merozoites invade erythrocytes and replicate within a parasitophorous vacuole to form daughter cells that eventually exit (egress) by sequential rupture of the vacuole and erythrocyte membranes. The current model is that PKG, a malarial cGMP-dependent protein kinase, triggers egress, activating malarial proteases and other effectors. Using selective inhibitors of either PKG or cysteine proteases to separately inhibit the sequential steps in membrane perforation, combined with video microscopy, electron tomography, electron energy loss spectroscopy, and soft X-ray tomography of mature intracellular Plasmodium falciparum parasites, we resolve intermediate steps in egress. We show that the parasitophorous vacuole membrane (PVM) is permeabilized 10-30 min before its PKG-triggered breakdown into multilayered vesicles. Just before PVM breakdown, the host red cell undergoes an abrupt, dramatic shape change due to the sudden breakdown of the erythrocyte cytoskeleton, before permeabilization and eventual rupture of the erythrocyte membrane to release the parasites. In contrast to the previous view of PKG-triggered initiation of egress and a gradual dismantling of the host erythrocyte cytoskeleton over the course of schizont development, our findings identify an initial step in egress and show that host cell cytoskeleton breakdown is restricted to a narrow time window within the final stages of egress

‘On the outside I’m smiling but inside I’m crying’: communication successes and challenges for undergraduate academic writing

Student difficulties with the transition to writing in higher education are well documented whether from a ‘study skills’, an ‘academic socialisation’ or an ‘academic literacies’ perspective. In order to more closely examine the challenges faced by students from widening participation backgrounds and diverse routes into undergraduate study, this project focuses on first-year undergraduate experiences of developing academic literacies on an Education Studies programme at one university in England. It highlights the impact of different support and guidance within and beyond their degree programme where attempts to embed academic literacy development are part of subject modules. The paper reports the findings generated using a mixed methods interpretive approach. Questionnaires were collected at the beginning (n = 48) and end of the students’ first year (n = 44), and interviews and visual data collection methods (n =19) were used at the mid-point of the academic year. Key findings highlight students’ expectations of achievement on entry to university and the influence of the emotional journey of students as they begin to make progress as academic writers. Identifying, selecting and applying academic reading were an enduring concern whilst some students struggled with the digital literacy implicit in undergraduate work. Importantly, some strategies developed to support student transition to academic writing in higher education may have unintended consequences as they progress through the first year

Processing of Plasmodium falciparum Merozoite Surface Protein MSP1 activates a Spectrin-binding function enabling parasite egress from RBCs

The malaria parasite Plasmodium falciparum replicates within erythrocytes, producing progeny merozoites that are released from infected cells via a poorly understood process called egress. The most abundant merozoite surface protein, MSP1, is synthesized as a large precursor that undergoes proteolytic maturation by the parasite protease SUB1 just prior to egress. The function of MSP1 and its processing are unknown. Here we show that SUB1-mediated processing of MSP1 is important for parasite viability. Processing modifies the secondary structure of MSP1 and activates its capacity to bind spectrin, a molecular scaffold protein that is the major component of the host erythrocyte cytoskeleton. Parasites expressing an inefficiently processed MSP1 mutant show delayed egress, and merozoites lacking surface-bound MSP1 display a severe egress defect. Our results indicate that interactions between SUB1-processed merozoite surface MSP1 and the spectrin network of the erythrocyte cytoskeleton facilitate host erythrocyte rupture to enable parasite egress