Níveis sanguíneos do cálcio, fósforo, magnésio e correlação entre calcemia e fosfatase alcalina, durante a prenhez da égua puro sangue inglês (P. S. I.)

In this work the blood serum levels of calcium, inorganic phosphorus, magnesium and correlation between calcemia and alkaline phosphatase during pregnancy of the thoroughbred mares have been carried out. Samples of blood have been collected from 12 mares in different periods of the pregnancy as well as between 10-30 days post partum. Total calcium and magnesium were determined by the TODD method; the inorganic phosphorus by the FISKE-SUBBAROW technic and the alkaline phosphatase by the KING ARMSTRONG technic. The mean values in mg % of the total calcium from the gestation periods of 230 to 280 days (X = 11,213), of 280 to 330 days (X = 11,155) and of 10 to 30 days after foaling (X = 11,051), have shown statiscal significance difference from the periods of 30 to 80 days (X = 11,857), of 80 to 130 days (X = 11,632), of 130 to 180 days (X = 11,845) and of 180 to 230 days (X = 11,935). As has been shown, pregnant mares, especially during the advanced stages of pregnancy, as well as some days after foaling, showed a tendency toward reduction in total blood serum calcium. The mean values in mg % of the inorganic phosphorus, indifferent periods of gestation were as follow: 30 to 80 days (X =5,475); 80 to 130 days (X = 6,366); 130 to 180 days (X = 5,866); 180 to 230 days (X = 5,558); 230 to 280 days (X = 5,341); 280 to 330 days (X = 5,933) and 10 to 30 days after foaling (X = 5,858). These results have not shown statistical significance difference among them. The mean values in mg % of the magnesium, in different periods of gestation, were as follow: 30 to 80 days (X = 2,086); 80 to 130 (X = 2,273); 130 to 180 (X = 2,218); 180 — 230 (X = 4,328); 230 to 280 (X = 2,448); 280 to 330 (X = 2,424) and 10 to 30 days after foaling (X = 2,195). Only the mean value from the period of 30 to 80 days has shown statistical significance from all the other ones. The mean values of the total calcium taken between 280 to 330 days of pregnancy and between 10 to 30 days after foaling, when correlated with the mean values of the blood serum alkaline phosphatase from the same periods of the pregnancy (see Rev. Fac. Med. Vet. S. Paulo, 8: 445, 1970), have shown statistical significance at the level of 5% (r = 0,83) by the Pearson correlation coefficient.O artigo apresenta resumo em inglês

Effect of pH of injection vehicle on the biological activity of pregnant mare serum (P. M. S.) and chorionic (H. C. G.) gonadotrophins on the weight of the ovary and uterus in immature rats

O artigo apresenta resumo em inglês.Systemic investigations on the effect of pH on the biological activity of pregnant mare serum and chorionic gonadotropins have been carried out using intact immature rats. With the P. M. S. the uterine and the ovarian weight responses showed the lowest values at pH 3 and pH 10 and highest values at pH 7, at moderate doses of hormones. However, the observed differences were not statistically significant. With the H.C.G. the uterine and ovarian weight responses showed the lowest values at pH 3 and highest values at pH 7 and pH 10. Significant difference in the ovarian weight response between pH 3 and those of the pH 7 — pH 10 could be demonstrated, whereas no significant difference was found with the results obtained in the uterine weights groups