Synthesis and evaluation of novel analogues of mangiferin as potent antipyretic

ObjectiveTo screen different analogues of mangiferin pharmacologically for antipyretic activity.MethodsThe naturally occurring xanthone glycoside mangiferin was isolated by column chromatography from the ethanolic extract of stem bark of Mangifera indica. Mangiferin was further converted to 5–(N–phenylamino methyleno) mangiferin, 5–(N–p–chlorophenylamino methyleno) mangiferin, 5–(N–2-methyl phenylamino methyleno) mangiferin, 5–(N–p–methoxy phenylamino methyleno) mangiferin, 5–(N, N–diphenylamino methyleno) mangiferin, 5–(N–α–napthylamino methyleno) mangiferin and 5–(N–4–methyl phenylamino methyleno) mangiferin analogues. The synthesized compounds were further screened for antipyretic activity along with mangiferin at a dose level of 100 and 200 mg/kg. Mangiferin and its analogues were characterized by melting point andRfvalue determination and through spectral technique like UV, IR, and NMR spectral analysis.ResultsThe antipyretic activity of mangiferin as well as all analogues was found to be more significant in at higher dose ie. 200 mg/kg which was depicted through a decrease in rectal temperature up to 3 h.ConclusionsThe antipyretic activity of mangiferin and its analogues may be attributed to inhibition in synthesis of TNF–α and anti-oxidant activity associated with amelioration of inflammatory actions of cytokines