Salt treatment can change composition of glycinin and β-conglycinin proteins in soybean seed

Abstract This study examined the effects of KCl and NaH 2 PO 4 on quality and quantity changes of soybean seed proteins. These salts affect on protein synthesis. Pot investigations were carried out with soybean [Glycine max (L.) Merr. cv. Pershing] grown under various levels of KCl (0, 15, 30, 45, and 60 g) and NaH 2 PO 4 (0, 0.02, 0.2, and 2 g) both per 100 kg soil. Changes in proteins were determined by SDS-PAGE, Bradford test, measurement of cysteine, total -SH group, and disulfide bonds content. Results showed differences between glycinin and β-conglycinin subunits, amount of total protein and cysteine, also the amount of free sulfur in various levels of KCl and NaH 2 PO 4 . The highest amount of sulfur and acidic subunit of glycinin containing more methionine and cysteine were observed in seeds of plants grown in the soil treated with 0.2 g of NaH 2 PO 4 with no KCl