RadoNorm – towards effective radiation protection based on improved scientific evidence and social considerations – focus on RADON and NORM

RadoNorm aims to manage risks from exposures to radon and naturally occurring radioactive material (NORM) to promote effective radiation protection based on improved scientific evidence and social considerations. It supports the European Member States and the EU Commission (EC) in implementing the Basic Safety Standards for protection against ionising radiation hazards at the legislative, executive, and operational levels (Directive 2013/59/EURATOM). The project is grounded on (1) implementation of multidisciplinary and innovative research and technologies, (2) integration of education and training, and (3) dissemination of project results targeting a broad stakeholder community including the public, regulators, and policymakers. The objectives are achieved through scientific research-related topics (exposure, dosimetry, biology, epidemiology, societal aspects), cross-cutting topics (education and training, dissemination, ethics) and project management. The project will yield guidelines at legal, executive and operational levels. It will enable consolidated and harmonised decision-making in the field of radiation protection, considering societal aspects and sustainable knowledge transfer. The project contributes to EC activities to strengthen radiation protection in a consistent and joint manner, as has already been done through the establishment of radiation protection platforms, the promotion of projects (e.g., DoReMi, OPERRA) and the partnership CONCERT-EJP. The outcomes may also impact future recommendations

Improved expertise in radiation protection, nuclear chemistry and geological disposal

In the past 5 years several projects were launched in FP7 and H2020 with the aim to support competence building in nuclear by fostering education and training (E&T) initiatives. ENETRAP III, CONCERT, CINCH II, MEET-CINCH, PETRUS and EAGLE deal with advanced E&T in the fields of radiation protection, nuclear chemistry and geological disposal and the transfer of basic knowledge about ionising radiation, its benefits and risks, to the general public. They were launched with the overall objective of maintaining and extending nuclear know-how and competences in Europe and ensuring sustainable knowledge transfer to current and future generations. This paper describes the aims and achievements of these projects and, based on insights and experiences from these projects, provides some recommendations for future policy support regarding maintaining competences in nuclear industry and research

Advanced Omics and Radiobiological Tissue Archives: The Future in the Past

Archival formalin-fixed, paraffin-embedded (FFPE) tissues and their related diagnostic records are an invaluable source of biological information. The archival samples can be used for retrospective investigation of molecular fingerprints and biomarkers of diseases and susceptibility. Radiobiological archives were set up not only following clinical performance such as cancer diagnosis and therapy but also after accidental and occupational radiation exposure events where autopsies or cancer biopsies were sampled. These biobanks provide unique and often irreplaceable materials for the understanding of molecular mechanisms underlying radiation-related biological effects. In recent years, the application of rapidly evolving “omics” platforms, including transcriptomics, genomics, proteomics, metabolomics and sequencing, to FFPE tissues has gained increasing interest as an alternative to fresh/frozen tissue. However, omics profiling of FFPE samples remains a challenge mainly due to the condition and duration of tissue fixation and storage, and the extraction methods of biomolecules. Although biobanking has a long history in radiation research, the application of omics to profile FFPE samples available in radiobiological archives is still young. Application of the advanced omics technologies on archival materials provides a new opportunity to understand and quantify the biological effects of radiation exposure. These newly generated omics data can be well integrated into results obtained from earlier experimental and epidemiological analyses to shape a powerful strategy for modelling and evaluating radiation effects on health outcomes. This review aims to give an overview of the unique properties of radiation biobanks and their potential impact on radiation biology studies. Studies recently performed on FFPE samples from radiobiology archives using advanced omics are summarized. Furthermore, the compatibility of archived FFPE tissues for omics analysis and the major challenges that lie ahead are discussed

RadoNorm – towards effective radiation protection based on improved scientific evidence and social considerations – focus on RADON and NORM

RadoNorm aims to manage risks from exposures to radon and naturally occurring radioactive material (NORM) to promote effective radiation protection based on improved scientific evidence and social considerations. It supports the European Member States and the EU Commission (EC) in implementing the Basic Safety Standards for protection against ionising radiation hazards at the legislative, executive, and operational levels (Directive 2013/59/EURATOM). The project is grounded on (1) implementation of multidisciplinary and innovative research and technologies, (2) integration of education and training, and (3) dissemination of project results targeting a broad stakeholder community including the public, regulators, and policymakers. The objectives are achieved through scientific research-related topics (exposure, dosimetry, biology, epidemiology, societal aspects), cross-cutting topics (education and training, dissemination, ethics) and project management. The project will yield guidelines at legal, executive and operational levels. It will enable consolidated and harmonised decision-making in the field of radiation protection, considering societal aspects and sustainable knowledge transfer. The project contributes to EC activities to strengthen radiation protection in a consistent and joint manner, as has already been done through the establishment of radiation protection platforms, the promotion of projects (e.g., DoReMi, OPERRA) and the partnership CONCERT-EJP. The outcomes may also impact future recommendations

The STORE platform for data and resource sharing in radiation biology, radioecology and epidemiology

The primary data produced in the course of publicly-funded science represents a common asset for society as much as the analysed and interpreted results. Recent years have seen widespread agreement that such data should be accessible by scientists and the public. Open access publication has been the focus of much attention. However, the public availability of primary data requires the establishment of governance and a sharing infrastructure. International guidelines have been established for open data (FAIR) and transparent publication (TOP). Availability and reuse of primary scientific data increases the accountability, reproducibility and value of publicly funded science and assures that research resources developed with public funds become readily available to the broader research community ultimately to the benefit of the public. Furthermore, journals and funders are increasingly requiring that all study data are made openly available. In response to these needs, in radiation protection research we have developed the STORE data sharing platform, initially funded under the European Commission’s EURATOM programme. STORE permits users to upload and share data; users can maintain control over data dissemination through Creative Commons licensing. The data structure in STORE is centred on the study which is a top level directory. Within a study there are datasets and within these there can be individual files or data elements, which can be of any type or size and annotated with standard metadata Each dataset and data item are assigned a STORE ID and a DOI. STORE currently contains about 100 studies and more than 3000 individual data elements, which range from epidemiology data, through images to proteomics and raw mass spectroscopy data. STORE is compliant with the FAIR data principles and is registered with r3Data, the ELIXIR/EBI-based MIRIAM register of persistent identifiers, and the FAIR sharing initiative. STORE (http://www.storedb.org) is open and free to investigators and to funding agencies as a central repository for data sharing

Trichomes of Tobacco Excrete Zinc as Zinc-Substituted Calcium Carbonate and Other Zinc-Containing Compounds

Tobacco (Nicotiana tabacum L. cv Xanthi) plants were exposed to toxic levels of zinc (Zn). Zn exposure resulted in toxicity signs in plants, and these damages were partly reduced by a calcium (Ca) supplement. Confocal imaging of intracellular Zn using Zinquin showed that Zn was preferentially accumulated in trichomes. Exposure to Zn and Zn + Ca increased the trichome density and induced the production of Ca/Zn mineral grains on the head cells of trichomes. These grains were aggregates of submicrometer-sized crystals and poorly crystalline material and contained Ca as major element, along with subordinate amounts of Zn, manganese, potassium, chlorine, phosphorus, silicon, and magnesium. Micro x-ray diffraction revealed that the large majority of the grains were composed essentially of metal-substituted calcite (CaCO(3)). CaCO(3) polymorphs (aragonite and vaterite) and CaC(2)O(4) (Ca oxalate) mono- and dihydrate also were identified, either as an admixture to calcite or in separate grains. Some grains did not diffract, although they contained Ca, suggesting the presence of amorphous form of Ca. The presence of Zn-substituted calcite was confirmed by Zn K-edge micro-extended x-ray absorption fine structure spectroscopy. Zn bound to organic compounds and Zn-containing silica and phosphate were also identified by this technique. The proportion of Zn-substituted calcite relative to the other species increased with Ca exposure. The production of Zn-containing biogenic calcite and other Zn compounds through the trichomes is a novel mechanism involved in Zn detoxification. This study illustrates the potential of laterally resolved x-ray synchrotron radiation techniques to study biomineralization and metal homeostasis processes in plants

Funding for radiation research: past, present and future

Purpose: For more than a century, ionizing radiation has been indispensable mainly in medicine and industry. Radiation research is a multidisciplinary field that investigates radiation effects. Radiation research was very active in the mid- to late 20th century, but has then faced challenges, during which time funding has fluctuated widely. Here we review historical changes in funding situations in the field of radiation research, particularly in Canada, European Union countries, Japan, South Korea, and the US. We also provide a brief overview of the current situations in education and training in this field. Conclusions: A better understanding of the biological consequences of radiation exposure is becoming more important with increasing public concerns on radiation risks and other radiation literacy. Continued funding for radiation research is needed, and education and training in this field are also important