E.denticulatum Quant Method Validation

Raw and processed MaxQaunt data (tryptic, semi-tryptic, and unspecific) from three replicates of E. coli whole cell lysates. The data is used to validate the methodological approach for length-normalized, relative protein quantification applied in the study of protein extracts from E. denticulatum (10.17632/y4kmnb3tvx.2)

The Canadian Society for Bioengineering La Société Canadienne de Génie Agroalimentaire et de Bioingénierie EXTRACTION OF GINSENOSIDES FROM AMERICAN GINSENG (PANAX QUINQUEFOLIUM L.) ROOT WITH DIFFERENT EXTRACTION METHODS AND CHROMATOGRAPHIC ANALYSIS OF THE

Abstract: Microwave-assisted extraction (MAE) was compared with room temperature extraction (RTE) and reflux temperature extraction (RFX) on the extraction of ginsenosides from fresh American ginseng root. Extraction times of 5, 10, 30 and 60 min were investigated. An 86 -300% increase in extraction rate was observed by raised temperature. The use of microwave energy instead of hotplate heating in the extraction resulted in a 31 -96% increase in extraction rate with the exception of ginsenoside Re

Impact of substrate moisture content on growth and performance of black soldier fly larvae

Experimental data and data analyses growth, respiration and performance of black soldier fly larvae grown at different substrate moisture content

Methonotrophic Biomass Quant BUP

Raw LC-MS/MS data and MaxQuant output files (txt folder) from quantitative bottom-up proteomics analysis of the fermeted biomass from methanotrophic bacteria described in the manuscript "Emulsifier peptides derived from seaweed, methanotrophic bacteria, and potato proteins identified by quantitative proteomics and bioinformatics". The biomass was pre-fractionated by SDS-PAGE and subsequently in-gel digested as described in M&M. The remaining two raw data files can be found in "Gregersen, Simon (2021), “Methonotrophic Biomass Quant BUP part2”, Mendeley Data, V1", doi: 10.17632/76v7mnmyr3.

CodfishHydrolysatesMS

MS raw data, MaxQuant output files, and Table S4 for the manuscript "Biofunctionality of enzymatically derived peptides from codfish (Gadus morhua) frame; Bulk in vitro properties, quantitative proteomics, and bioinformatic prediction

UPLIFT Plastic Upcycling project

This repository will collect the datasets, public deliverables and published articles of the UPLIFT project, funded European Union’s Horizon 2020 research and innovation programme under grant agreement Nº 953073

Methonotrophic Biomass Quant BUP part2

Raw LC-MS/MS data and MaxQuant output files (txt folder) from quantitative bottom-up proteomics analysis of the fermeted biomass from methanotrophic bacteria described in the manuscript "Emulsifier peptides derived from seaweed, methanotrophic bacteria, and potato proteins identified by quantitative proteomics and bioinformatics". The biomass was pre-fractionated by SDS-PAGE and subsequently in-gel digested as described in M&M. This is the second part of the dataset for the manuscript. The former set can be found in "Gregersen, Simon (2021), “Methonotrophic Biomass Quant BUP”, Mendeley Data, V1", doi: 10.17632/g45gbw5r7n.

E.denticulatum quant BUP

MaxQuant output files (txt folder) from quantitative bottom-up proteomics analysis of two pilot-scale hot-water extracts from E. denticulatum, described in the manuscript "Proteomic characterization of pilot scale hot-water extracts from the industrial carrageenan red seaweed Eucheuma denticulatum" Extract A is labelled "A-013-05e" and Extract B "A-022-05e". Data is organized as exploratory phase (unspecific analysis with 1%, 5%, and 10% FDR as well as semi-specific analysis with 1% and 5% FDR) and as final phase (for tryptic and semi-specific analysis with optimized search parameters). Raw MS data are available upon request. Repository also contains the reference protein database (in .fasta format) as well as qRNAseq data

Gigartina pilot scale protein extract BUP

Seaweeds are attracting substantial interest as novel sources of sustainable food protein, as they are established sources of industrial hydrocolloids and have reasonable protein content. In this study we investigate the protein composition and quality of a seaweed protein extract (SPE) from Gigartina radula obtained as a side-stream from industrial carrageenan production. The SPE displayed low (<2%), but pH-dependent, aqueous solubility due to the harsh (heat and extreme pH) employed during extraction. Solubility was improved using buffer and detergent to facilitate proteomic characterization by quantitative LC-MS/MS. Proteomics revealed that the SPE was dominated by proteins related to light harvest and particularly rich in phycobiliproteins (44% relative molar abundance), where phycoerythrin was the most abundant (28%). Based on subcellular localization of identified proteins, the extraction method was evaluated as good for release of cellular protein. The SPE was found to be rich in essential amino acids (36-41%) and particularly in branched chain amino acids (22-24%) and thereby a potential source of nutritional food protein. Using bioinformatic prediction and structural modelling, we found that abundant proteins in the SPE contained high potential, novel emulsifier peptides with amphiphilic properties required to stabilize an oil/water interface. Based on this study, Gigartina could serve as a good candidate for extraction of sustainable and nutritious food protein and possibly be further processed to obtain a hydrolysate with good emulsifying properties for use in foods

Figure 3 from: Cannon P, Buddie A, Bridge P, de Neergaard E, Lübeck M, Askar M (2012) Lectera, a new genus of the Plectosphaerellaceae for the legume pathogen Volutella colletotrichoides. MycoKeys 3: 23-36. https://doi.org/10.3897/mycokeys.3.3065

Figure 3 - ML tree of GAPDH sequences, of a subset of the strains used for the ITS sequence set