Proteomics and network pharmacology of Ganshu Nuodan capsules in the prevention of alcoholic liver disease

IntroductionGanshu Nuodan is a liver-protecting dietary supplement composed of Ganoderma lucidum (G. lucidum) spore powder, Pueraria montana (Lour.) Merr. (P. montana), Salvia miltiorrhiza Bunge (S. miltiorrhiza) and Astragalus membranaceus (Fisch.) Bunge. (A. membranaceus). However, its pharmacodynamic material basis and mechanism of action remain unknown.MethodsA mouse model of acute alcohol liver disease (ALD) induced by intragastric administration of 50% alcohol was used to evaluate the hepatoprotective effect of Ganshu Nuodan. The chemical constituents of Ganshu Nuodan were comprehensively identified by UPLC-QTOF/MS, and then its pharmacodynamic material basis and potential mechanism of action were explored by proteomics and network pharmacology.ResultsGanshu Nuodan could ameliorate acute ALD, which is mainly manifested in the significant reduction of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum and malondialdehyde (MDA) content in liver and the remarkably increase of glutathione (GSH) content and superoxide dismutase (SOD) activity in liver. Totally 76 chemical constituents were identified from Ganshu Nuodan by UPLC-QTOF/MS, including 21 quinones, 18 flavonoids, 11 organic acids, 7 terpenoids, 5 ketones, 4 sterols, 3 coumarins and 7 others. Three key signaling pathways were identified via proteomics studies, namely Arachidonic acid metabolism, Retinol metabolism, and HIF-1 signaling pathway respectively. Combined with network pharmacology and molecular docking, six key targets were subsequently obtained, including Ephx2, Lta4h, Map2k1, Stat3, Mtor and Dgat1. Finally, these six key targets and their related components were verified by molecular docking, which could explain the material basis of the hepatoprotective effect of Ganshu Nuodan.ConclusionGanshu Nuodan can protect acute alcohol-induced liver injury in mice by inhibiting oxidative stress, lipid accumulation and apoptosis. Our study provides a scientific basis for the hepatoprotective effect of Ganshu Nuodan in acute ALD mice and supports its traditional application

Research Progress on Peony under High Temperature Stress Caused by Climate Warming

Global warming and worsening environmental problems have worsened the heat resistance of peony and difficult maintenance and management. The high temperature stress caused by the environment has become an important environmental factor for the growth and development of peony. The article summarizes the morphology, yield, physiological and biochemical indicators and research status of peony under high temperature stress; summarizes the research status of peony heat tolerance evaluation index screening, germplasm heat tolerance evaluation and heat-resistant peony breeding, in order to promote tolerance Selection and breeding process of hot peony germplasm resources. At present, related researches on peony mainly focus on pharmacological effects and germplasm resources. The physiological mechanism of high temperature and breeding of heat-resistant varieties of peony are relatively lagging behind. In-depth study of the physiological mechanism of high temperature in peony combined with multi-omics will help to adopt technical measures to improve the heat tolerance of high plants and reduce heat damage, so as to speed up the selection and breeding of heat-resistant peony germplasm, and meet the survival and medicine of peony in harsh environments. Use and other needs

Seasonal and Intra-Annual Patterns of Sedimentary Evolution in Tidal Flats Impacted by Laver Cultivation along the Central Jiangsu Coast, China

Human activities such as the rapid development of marine aquaculture in the central Jiangsu coast have had a marked impact on the tidal flat morphology. This research focuses on characterizing the spatial expansion of laver cultivation and its influence on the sedimentary evolution of tidal flats in the central Jiangsu coast. First, seasonal digital elevation models (DEMs) were established using 160 satellite images with medium resolution. Then, laver aquaculture regions were extracted from 50 time-series satellite images to calculate the area and analyze the spatial distribution and expansion of these areas. Finally, seasonal and intra-annual sedimentary evolution patterns of both aquaculture and non-aquaculture regions were determined using the constructed DEMs. Our results show that aquaculture regions have gradually expanded to the north and peripheral domains of the entire sand ridge since 1999 and by 2013, the seaward margins of each sandbank developed into dense cultivation regions. Additionally, the aquaculture regions increased from 11.99 km2 to 295.28 km2. The seasonal sedimentary evolution patterns indicate that deposition occurs during the winter and erosion during the summer. Thus, the aquaculture regions experience deposition in certain elevation intervals during the laver growing period and in the non-growing period, alluvial elevation intervals in the aquaculture regions are eroded and erosive ones are deposited in order to maintain the balance between scouring and silting. The sedimentary evolution of each sandbank is heterogeneous due to their different locations and the difference in sediment transport. The intra-annual evolution pattern is characterized by deposition in the high tidal flats and erosion in low ones. Hydrodynamic conditions and laver cultivation dominate partial sedimentary evolution, which gradually shapes the beach surface

Role of MMP-2(-1306 C/T) and TIMP-2(-418G/C) Polymorphism in Chinese Han Patients with Acne Vulgaris

Acne is the most common chronic inflammatory skin diseases. Multiple factors, such as hormonal, environmental, immunological, and genetic factors, are thought to be involved in acne. However, genetic studies have yet to elucidate the full mechanism of acne. The aim of this study was to investigate the association of MMP-2 (-1306C/T) and TIMP-2 (-418G/C) polymorphisms with the risk of acne vulgaris in a Chinese Han population. We also analyzed the correlation of clinical parameters and family history in patients with acne vulgaris. This study included 251 acne patients and 121 age- and sex-matched healthy controls. Genomic DNA was extracted from peripheral blood, and genotyping was performed by PCR and DNA sequencing techniques. There is a significant correlation between the MMP-2 (-1306C/T) polymorphism and the acne vulgaris (P<0.001). Although no association was found between the TIMP-2 (-418G/C) polymorphism and the acne vulgaris, patients with the MMP-2 CT/TIMP-2 GG or GC allele are at higher risk of acne vulgaris. There is also a significant difference in the severity of the disease between acne vulgaris patients with and without family history (P<0.001). This study indicated that the MMP-2 (-1306C/T) polymorphism, in combination with the TIMP-2 (-418G/C) polymorphism, contributes to acne vulgaris susceptibility in the Chinese Han population

Morphological Characteristics of Tidal Creeks in the Central Coastal Region of Jiangsu, China, Using LiDAR

Tidal creeks are an important component of the intertidal zone and are essential for maintaining the balance between sedimentary processes and the hydrodynamic environment. A quantitative analysis of the morphological characteristics of tidal creeks is essential for understanding their processes of evolution and to evaluate the stability of tidal flats. This study describes the morphological characteristics of tidal creeks using a high-resolution airborne LiDAR DEM. The parameters include the number, order, length, width, depth, and width/depth ratio. The results show that the number and degree of development tidal creeks along the central coast of Jiangsu are higher than those in the radial sandbanks, and the mean width and length increase with the increasing tidal creek order. The number, length, and depth of tidal creeks in the salt marsh zone with well-developed vegetation are higher than those areas with little vegetation cover. The number of tidal creeks in the mid-upper intertidal zone is the largest, while the length and width of tidal creeks in the lower intertidal zone are the greatest. The differences in these characteristics are mainly related to the vegetation distribution, tidal flat width, and hydrodynamic conditions. Our findings potentially provide guidelines for coastal management and the evaluation of tidal flat stability

Understanding the Spatial-Temporal Patterns and Influential Factors on Air Quality Index: The Case of North China

North China has become one of the worst air quality regions in China and the world. Based on the daily air quality index (AQI) monitoring data in 96 cities from 2014&ndash;2016, the spatiotemporal patterns of AQI in North China were investigated, then the influence of meteorological and socio-economic factors on AQI was discussed by statistical analysis and ESDA-GWR (exploratory spatial data analysis-geographically weighted regression) model. The principal results are as follows: (1) The average annual AQI from 2014&ndash;2016 exceeded or were close to the Grade II standard of Chinese Ambient Air Quality (CAAQ), although the area experiencing heavy pollution decreased. Meanwhile, the positive spatial autocorrelation of AQI was enhanced in the sample period. (2) The occurrence of a distinct seasonal cycle in air pollution which exhibit a sinusoidal pattern of fluctuations and can be described as &ldquo;heavy winter and light summer.&rdquo; Although the AQI generally decreased in other seasons, the air pollution intensity increased in winter with the rapid expansion of higher AQI value in the southern of Hebei and Shanxi. (3) The correlation analysis of daily meteorological factors and AQI shows that air quality can be significantly improved when daily precipitation exceeds 10 mm. In addition, except for O3, wind speed has a negative correlation with AQI and major pollutants, which was most significant in winter. Meanwhile, pollutants are transmitted dynamically under the influence of the prevailing wind direction, which can result in the relocation of AQI. (4) According to ESDA-GWR analysis, on an annual scale, car ownership and industrial production are positively correlated with air pollution; whereas increase of wind speed, per capita gross domestic product (GDP), and forest coverage are conducive to reducing pollution. Local coefficients show spatial differences in the effects of different factors on the AQI. Empirical results of this study are helpful for the government departments to formulate regionally differentiated governance policies regarding air pollution

Controlling Shear Rate for Designable Thermal Conductivity in Direct Ink Printing of Polydimethylsiloxane/Boron Nitride Composites

Efficient heat dissipation is vital for advancing device integration and high-frequency performance. Three-dimensional printing, famous for its convenience and structural controllability, facilitates complex parts with high thermal conductivity. Despite this, few studies have considered the influence of shear rate on the thermal conductivity of printed parts. Herein, polydimethylsiloxane/boron nitride (PDMS/BN) composites were prepared and printed by direct ink writing (DIW). In order to ensure the smooth extrusion of the printing process and the structural stability of the part, a system with 40 wt% BN was selected according to the rheological properties. In addition, the effect of printing speed on the morphology of BN particles during 3D printing was studied by XRD, SEM observation, as well as ANSYS Polyflow simulation. The results demonstrated that increasing the printing speed from 10 mm/s to 120 mm/s altered the orientation angle of BN particles from 78.3° to 35.7°, promoting their alignment along the printing direction due to the high shear rate experienced. The resulting printed parts accordingly exhibited an impressive thermal conductivity of 0.849 W∙m−1∙K−1, higher than the 0.454 W∙m−1∙K−1 of the control sample. This study provides valuable insights and an important reference for future developments in the fabrication of thermal management devices with customizable thermal conductivity

Effects of Lactic Acid Bacteria Inoculants and Stage-Increased Storage Temperature on Silage Fermentation of Oat on the Qinghai&ndash;Tibet Plateau

Ensiling is a simple and effective method of alleviating a shortage of forage for ruminants. This study aimed to investigate the effects of lactic acid bacteria (LAB) inoculants and stage-increased temperature on the fermentation characteristics and chemical composition of oat silage on the Qinghai&ndash;Tibet Plateau. The silage was treated with local laboratory inoculant (I) and commercial inoculant (S) and stored at ambient temperature (&lt;10 &deg;C) or stage-increased (5, 10 and 15 days) temperatures of 10 &deg;C and 15 &deg;C for 60 days. The results showed that stage-increased storage temperature can improve silage fermentation. Compared with 10 &deg;C, a stage-increased storage temperature of 15 &deg;C effectively (p &lt; 0.05) promoted the fermentation rate of silage by increasing the dominance of Lactiplantibacillus plantarum, with higher lactic, acetic and propionic acid contents and a lower ammonia-N ratio of the total N and final pH value. Compared with S, treatment with I increased the water-soluble carbohydrate and lactic acid contents and decreased the ammonia-N ratio of the total N and final pH value. This work demonstrated that increasing the storage temperature in stages using a warming infrastructure facilitates the preservation of oat silage in cold regions, and the inoculation of lactic acid bacteria could advance silage fermentation on the Qinghai&ndash;Tibet Plateau

REC8 regulates neuroblastoma cell proliferation, migration, invasion, and angiogenesis via STAT3/VEGF signaling

Abstract Background Neuroblastoma, one of the most prevalent childhood cancers, is often treated with surgery, radiation, and chemotherapy. However, prognosis and survival are still dismal for children with neuroblastoma at high risk. Consequently, it is vital to identify new and effective treatment targets. As a component of the meiotic cohesion complex, REC8 is involved in a wide range of malignancies. The current work assessed the impact of REC8 knockdown on SH-SY5Y and SK-N-AS neuroblastoma cells and delved into the molecular mechanism behind this effect. Methods Knockdown of REC8 using the small interfering (si) RNA technology, and the results were verified by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and western blot. The Cell Counting Kit-8 (CCK-8) was used to examine cell proliferation, while flow cytometry was used to examine cell cycle progression and apoptosis. Analyses of angiogenesis included tube formation experiments. Transwell tests were used to examine cell migration and invasion. Results The data showed that downregulation of the REC8 led to a substantial decrease in cell proliferation by stopping the cell cycle in the G1 phase. REC8 knockdown significantly reduced neuroblastoma cell proliferation, migration, invasion, angiogenesis, induced cell cycle arrest, and enhanced apoptosis. We also discovered that repressing REC8 expression in neuroblastoma cell lines SH-SY5Y and SK-N-AS reduced their ability to activate the STAT3/VEGF signaling pathway. Conclusions Neuroblastoma therapy may benefit from targeting REC8 and its downstream targets