Proizvodnja egzopolisaharida i sprečavanje sinereze škroba pomoću enkapsuliranoga probiotika Lactobacillus plantarum

Encapsulation of probiotic bacteria with a matrix can increase their survival rate by protecting them from adverse conditions and at the same time without affecting the production of metabolites. An effort has been made to encapsulate the probiotic Lactobacillus plantarum using calcium alginate. Box-Behnken model of response surface methodology (RSM) was employed in the optimization of major encapsulation conditions such as concentration of sodium alginate, calcium chloride and curing time. The second-order quadratic model with the optimum conditions (sodium alginate 2 % (by mass per volume), calcium chloride 0.5 M and curing time 3 h) resulted in a maximum titre of (0.9±0.1) g/L of exopolysaccharides (EPS) at 72 h. The nearness of the coefficient of determination (R²=0.97) to 1 ensures the satisfactory adjustment of the quadratic model to the experimental data. The efficiency of EPS production by encapsulated cells was compared with free cells. The efficacy of secreted EPS in the prevention of syneresis in starch was investigated.Enkapsulacija probiotičkih bakterija povećava mogućnost njihova preživljavanja, štiteći ih od nepovoljnih uvjeta, bez utjecanja na proizvodnju metabolita. U radu se pokušalo enkapsulirati probiotičku bakteriju Lactobacillus plantarum pomoću kalcijeva alginata. Da bi se optimirali glavni uvjeti enkapsulacije, kao što su koncentracija natrijeva alginata, kalcijeva klorida i vrijeme tretiranja, primijenjen je Box-Behnkenov model metode odzivnih površina. Maksimalni titar egzopolisaharida od (0,9±0,1) g/L postignut je nakon 72 sata, i to uvrštavanjem optimalnih uvjeta (koncentracija natrijeva alginata od 2 % (m/V), kalcijeva klorida od 0,5 M i vrijeme tretiranja od 3 sata) u kvadratni model drugoga reda. Koeficijent determinacije od skoro 1 (R²=0,97) osigurao je zadovoljavajuću prilagodbu eksperimentalnih podataka kvadratnom modelu. Učinkovitost proizvodnje egzopolisaharida pomoću enkapsuliranih stanica uspoređena je s onom slobodnih stanica. Također je ispitana učinkovitost izlučenih egzopolisaharida u sprečavanju sinereze škroba

Exopolysaccharide Production and Prevention of Syneresis in Starch Using Encapsulated Probiotic Lactobacillus plantarum

Encapsulation of probiotic bacteria with a matrix can increase their survival rate by protecting them from adverse conditions and at the same time without affecting the production of metabolites. An effort has been made to encapsulate the probiotic Lactobacillus plantarum using calcium alginate. Box-Behnken model of response surface methodology (RSM) was employed in the optimization of major encapsulation conditions such as concentration of sodium alginate, calcium chloride and curing time. The second-order quadratic model with the optimum conditions (sodium alginate 2 % (by mass per volume), calcium chloride 0.5 M and curing time 3 h) resulted in a maximum titre of (0.9±0.1) g/L of exopolysaccharides (EPS) at 72 h. The nearness of the coefficient of determination (R²=0.97) to 1 ensures the satisfactory adjustment of the quadratic model to the experimental data. The efficiency of EPS production by encapsulated cells was compared with free cells. The efficacy of secreted EPS in the prevention of syneresis in starch was investigated