Nicotine Protects Kidney from Renal Ischemia/Reperfusion Injury through the Cholinergic Anti-Inflammatory Pathway

Kidney ischemia/reperfusion injury (I/R) is characterized by renal dysfunction and tubular damages resulting from an early activation of innate immunity. Recently, nicotine administration has been shown to be a powerful inhibitor of a variety of innate immune responses, including LPS-induced toxaemia. This cholinergic anti-inflammatory pathway acts via the α7 nicotinic acetylcholine receptor (α7nAChR). Herein, we tested the potential protective effect of nicotine administration in a mouse model of renal I/R injury induced by bilateral clamping of kidney arteries. Renal function, tubular damages and inflammatory response were compared between control animals and mice receiving nicotine at the time of ischemia. Nicotine pretreatment protected mice from renal dysfunction in a dose-dependent manner and through the α7nAChR, as attested by the absence of protection in α7nAChR-deficient mice. Additionally, nicotine significantly reduced tubular damages, prevented neutrophil infiltration and decreased productions of the CXC-chemokine KC, TNF-α and the proinflammatory high-mobility group box 1 protein. Reduced tubular damage in nicotine pre-treated mice was associated with a decrease in tubular cell apoptosis and proliferative response as attested by the reduction of caspase-3 and Ki67 positive cells, respectively. All together, these data highlight that nicotine exerts a protective anti-inflammatory effect during kidney I/R through the cholinergic α7nAChR pathway. In addition, this could provide an opportunity to overcome the effect of surgical cholinergic denervation during kidney transplantation

Contribution à l'étude phytochimique et pharmacologique du rhizome de cochlosperum tinctorium A. Rich. (Cochlospermaceae)

Doctorat en sciences pharmaceutiquesinfo:eu-repo/semantics/nonPublishe

Contribution à l'étude phytochimique et pharmacologique du rhizome de cochlosperum tinctorium A. Rich. (Cochlospermaceae)

Doctorat en sciences pharmaceutiquesinfo:eu-repo/semantics/nonPublishe

Apocarotenoids from Cochlospermum tinctorium

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Large scale purification of apocarotenoids from cochlospermum t1nctorium by counter-current chromatography

Carotenoids are well-known for their instability so as usual analytical methods such as HPLC or preparative TLC applied to the separation of the constituents of Cochlospermum tinctorium failed to the isolation of pure pigments for further chemical characterization. Thus high-speed CCC using a horizontal flow-through coil planet centrifuge was used to conveniently and efficiently achieve this separation for quantities up to 500mg of the crude plant extract. © 1988, Taylor & Francis Group, LLC. All rights reserved.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Plant Constituents Involved in Coating Practices among Traditional African Potters

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Separation of quassinoids from Ailanthus altissima by high-speed counter-current chromatography

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Triacylbenzenes and long-chain volatile ketones from Cochlospermum tinctorium rhizome

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Direct coupling of high-speed counter-current chromatography to thin-layer chromatography. Application to the separation of asiatocoside and madecassoside from Centella asiatica

The direct coupling of high-speed counter-current chromatography to thin-layer chromatography is proposed as an efficient method for the on-line monitoring of the column effluent. It requires a simple adaptation of the fittings of a Linomat C device without modification of the software. An application to the separation of asiaticoside aned madecassoside from a crude extract of Centella asiatica is presented.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Protein changes in Dioscorea alata during in vitro dormancy and germination of microtubers

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