Nitric oxide biosensor uncovers diminished ferrous iron-dependency of cultured cells adapted to physiological oxygen levels

Iron is an essential metal for cellular metabolism and signaling, but it has adverse effects in excess. The physiological consequences of iron deficiency are well established, yet the relationship between iron supplementation and pericellular oxygen levels in cultured cells and their downstream effects on metalloproteins has been less explored. This study exploits the metalloprotein geNOps in cultured HEK293T epithelial and EA.hy926 endothelial cells to test the iron-dependency in cells adapted to standard room air (18 kPa O2) or physiological normoxia (5 kPa O2). We show that cells in culture require iron supplementation to activate the metalloprotein geNOps and demonstrate for the first time that cells adapted to physiological normoxia require significantly lower iron compared to cells adapted to hyperoxia. This study establishes an essential role for recapitulating oxygen levels in vivo and uncovers a previously unrecognized requirement for ferrous iron supplementation under standard cell culture conditions to achieve geNOps functionality.Integration Projects of Sabanci University ; Heart Research U.K. ; British Heart Foundation ; European Cooperation in Science and Technology (COST) ; King's Together Strategic Awar

Corrigendum to "Nitric oxide biosensor uncovers diminished ferrous iron-dependency of cultured cells adapted to physiological oxygen levels" [Redox Biol. 53 (2022) 102319] (Redox Biology (2022) 53, (S221323172200091X), (10.1016/j.redox.2022.102319))

The authors regret that the cited reference list in the online PDF of this article contains a duplicate reference (Ref 34 and incomplete Ref 56). Ref 56 should read as follows: Sies, H. et al. Defining roles of specific reactive oxygen species (ROS) in cell biology and physiology. Nat Rev Mol Cell Biol (2022) Feb 21 https://doi.org/10.1038/s41580-022-00456-z.The authors would like to apologise for any inconvenience caused

Corrigendum to “Nitric oxide biosensor uncovers diminished ferrous iron-dependency of cultured cells adapted to physiological oxygen levels” [Redox Biol. 53 (2022) 102319] (Redox Biology (2022) 53, (S221323172200091X), (10.1016/j.redox.2022.102319))

The authors regret that the cited reference list in the online PDF of this article contains a duplicate reference (Ref 34 and incomplete Ref 56). Ref 56 should read as follows