Direct Waste-Free Synthesis of Amides from Nonactivated Carboxylic Acids and Amines: Application to the Synthesis of Tetrahydroisoquinolines

<div><p></p><p>An efficient, waste-free protocol has been developed for the synthesis of amides directly from nonactivated carboxylic acids and amines without any tedious purification. Fatty acids were also converted into corresponding amides by reaction with nonactivated amines. The synthesized amides were cyclized to tetrahydroisoquinolines in good yields.</p></div

Water-Mediated Synthesis of Benzazole and Thiourea Motifs by Reacting Naturally Occurring Isothiocyanate with Amines

<div><p></p><p>An efficient, green, and facile method has been developed for the synthesis of benzazole and thiourea analogues from naturally occurring erucin in moderate to good yields. The reaction was carried out in water without using any metal catalyst or base. The present method tolerated the various functional groups on aromatic rings and also applicable for other isothiocyanates.</p></div

Locational comparison of essential oils from selected conifers of Himachal Pradesh

<p>Nine samples of essential oil from needles of three conifers of Pinacea family namely <i>Abies pindrow</i>, <i>Picea smithiana</i> and <i>Cedrus deodara</i> collected from three different locations of Himachal Pradesh (India) were evaluated using gas chromatography and gas chromatography-mass spectrometry. A total of 31, 17 and 13 compounds were identified from essential oil of <i>A</i>. <i>pindrow</i>, <i>P</i>. <i>smithiana</i> and <i>C</i>. <i>deodara</i>, respectively. Among the characterised components, monoterpenoid hydrocarbons were predominated. <i>α</i>-Pinene, <i>β</i>-pinene, <i>β</i>-merycene, limonene and camphene were characterised as major components. Oil of <i>C</i>. <i>deodara</i> has significant effect of location on its oil composition. Principle component analysis on gas chromatographic data reveals variation in chemical composition which may be attributed to altitude and environmental conditions.</p

Chemical composition and insecticidal activities of essential oils against diamondback moth, <i>Plutella xylostella</i> (L.) (Lepidoptera: Yponomeutidae)

<p>Five Himalayan plants namely, <i>Acorus calamus</i>, <i>Cedrus deodara</i>, <i>Aegle marmelos</i>, <i>Tagetes minuta</i> and <i>Murraya koenigii</i> were used for the extraction of essential oils through hydrodistillation and the major volatile constituents as identified by GC and GC–MS techniques were β-asarone (91.1%), β-himachalene (45.8%), limonene (59.5%), <i>Z</i>-ocimene (37.9%) and α-pinene (54.2%), respectively. Essential oils were tested for their insecticidal properties against larvae of diamondback moth, <i>Plutella xylostella</i> (L.) (Lepidoptera: Yponomeutidae). Results showed that <i>A. calamus</i> was most toxic (LC₅₀= 0.29 mg mL⁻¹) to <i>P. xylostella</i> followed by <i>C. deodara</i> (LC₅₀= 1.08 mg mL⁻¹) and <i>M. koenigii</i> (LC₅₀= 1.93 mg mL⁻¹) via residual toxicity bioassay. Per cent feeding deterrence index and growth inhibition was significantly higher in <i>A. calamus</i> (42.20 and 68.55, respectively) followed by <i>C. deodara</i> (35.41 and 52.47). In repellent activity studies, <i>C. deodara</i> showed high repellence (64.76%) followed by <i>A. calamus</i> (55.05%).</p

Cytotoxic agents for KB and SiHa cells from <i>n</i>-hexane fraction of <i>Cissampelos pareira</i> and its chemical composition

<div><p>Eleven constituents were characterised by gas chromatography–mass spectrometry analysis, and five molecules were isolated using column chromatography. The <i>in vitro</i> study of the extract and isolated molecules against KB and SiHa cell lines revealed oleanolic acid (<b>1</b>) and oleic acid (<b>2</b>) as potent cytotoxic molecules with potential anticancer activity. The IC₅₀ values of <i>n</i>-hexane extract (CPHF), oleanolic acid (<b>1</b>) and oleic acid (<b>2</b>) were >300, 56.08 and 70.7 μg/mL (μM), respectively, against KB cell lines and >300, 47.24 and 80.2 μg/mL (μM), respectively, against SiHa cell lines.</p></div

Movement activities of children in their presschool age

v angličtině The name of dissertation: Movement activities of chlidren in their presschool age Written by: Šárka Kochanová The head of dissertation: PaedDr Jana Kolčiterová The objectives of dissertation: 1. Analysing some conditions for carrying out preschool children's activities at selected nursery schools and integrating individual preathletic exercises in chlidren's exercise. 2. Finding out parent's interests in children's sport activities. 3. Differertiation varios levels of children's sport activities at same age. Methodology: Study in depth of expert literaturee Informing about background at selected nursery schools Monitoring children's sport activities including preathletic exercises and testing its opinion Applycating simply athletic games to get necessary information to find out the differences in motor level of children at same age. Monitoring differences in sport activities of children living in town and in the country By way of unstandard questionnaire getting new information about children's and parent's attitudes to own assessment of their sport activities Results of dissertation 1. Conditions for sport activities at nursery schools locating in towns and in the country differ from one another. Nursery schools in the country don't measure up to the equipment of nursery schools in..

Additional file 4: Figure S1. of Association of SNP Haplotypes of HKT Family Genes with Salt Tolerance in Indian Wild Rice Germplasm

Linkage Disequilibrium plots for HKT genes Figure S2. Q-Q plots of HKT genes obtained after MLM based association of SNP with traits. (PDF 1156 kb

Additional file 2: Table S2. of Association of SNP Haplotypes of HKT Family Genes with Salt Tolerance in Indian Wild Rice Germplasm

Phenotype (SES) score and evaluated salt related traits among Indian wild rice at 10th day of 150mM salt treatment. (CSV 3 kb

Additional file 1: Table S1. of Association of SNP Haplotypes of HKT Family Genes with Salt Tolerance in Indian Wild Rice Germplasm

 List of wild rice accessions, their place of collection and Standard Evaluation System (SES) score obtained during screening. (CSV 13 kb

Bioactive isoquinoline alkaloids from <i>Cissampelos pareira</i>^†

<p>The phytochemical and biological investigation of <i>Cissampelos pareira</i> leads to the isolation of one new isoquinoline alkaloid (<b>7</b>) along with six known isoquinoline alkaloids, namely, magnoflorine (<b>1</b>), magnocurarine (<b>2</b>), cissamine (<b>3</b>), curine (<b>4</b>), hayatinine (<b>5</b>) and cycleanine (<b>6</b>). Magnoflorine (<b>1</b>) and magnocurarine (<b>2</b>) were isolated for the first time from <i>C. pareira.</i> A new, rapid, simple and sensitive UPLC method was developed for simultaneous quantification of five pure compounds (<b>1</b>–<b>5</b>). Seasonal variation study revealed higher content of these compounds during the rainy season. The chloroform (CPCF) and <i>n</i>-butanol (CPBF) fractions showed cytotoxic efficacy against KB cells. Among pure compounds, hayatinine (<b>5</b>) was found to be most active against KB and A549, while, cycleanine (<b>6</b>) against KB cells.</p