Multi-Electrode Microfluidic Platform for Protein Detection Using Electrochemical Impedance Spectroscopy

An impedimetric sensor was developed based on parallel detection at two identical microfluidic channels with 3 pairs of interdigitated electrodes in each. Preparation and cleaning of the gold electrodes were examined and optimized. A self-assembling monolayer of 11-mercaptopropionic acid was deposited to form a base for later covalent binding of antibody proteins via EDC/NHS chemistry. Two immunoassays containing immune reaction of a 214D4 antibody and a corresponding MUC1ex antigen and a prostate antigen antibody immunoassay with positive and negative controls were performed in two parallel microfluidic channels. Presented platform enables a simultaneous analysis of the protein reactions on multiple electrodes leading to an improvement in on-time diagnostics and point-of-care