A new dabco-templated metal sulfate: 1,4-diazo­niabicyclo­[2.2.2]octane hexa­aqua­cadmium bis­(sulfate)

The title double mol­ecular salt, (C6H14N2)[Cd(H2O)6](SO4)2, is an isostructure of its Mn and Co analogues. The CdII atom adopts a near-regular CdO6 octa­hedral coordination geometry. The crystal structure can be described as an alternation of cationic and anionic layers along [010], and numerous O—H⋯O and N—H⋯O hydrogen bonds are observed. No thermal anomalies corresponding to possible phase transitions were observed in DSC (differential scanning calorimetry) measurements and the 93 K structure is almost the same as the room-temperature structure

Interlayer Coupling of Co/NM/FM(NiFe and Co) Nano-Sandwich Films

AbstractCu/Co, Cu/NiFe, Ta/NiFe bilayers and Co/Cu/Co, Co/Cu/NiFe, Co/Ta/NiFe sandwich films were deposited by a magnetron sputtering method. Magnetic properties were evaluated by VSM and spin valve magnet oresistance was investigated by a four-probe method to study the interlayer coupling of the two magnetic layers. It has been found that the interlayer coupling depended not only on the layer thickness of the nonmagnetic spacer but also on the nature of the spacer. The interlayer coupling was reduced as the spacer layer thickness increased. The result was consistent with those from observations of the magnetic domain for the trilayers by means of Lorentz Electron Microscope. The trilayers with Cu spacer layer have shown a stronger coupling than those with Ta spacer layer

Mutations in an Atypical TIR-NB-LRR-LIM Resistance Protein Confer Autoimmunity

In order to defend against microbial infection, plants employ a complex immune system that relies partly on resistance (R) proteins that initiate intricate signaling cascades upon pathogen detection. The resistance signaling network utilized by plants is only partially characterized. A genetic screen conducted to identify novel defense regulators involved in this network resulted in the isolation of the snc6-1D mutant. Positional cloning revealed that this mutant contained a molecular lesion in the chilling sensitive 3 (CHS3) gene, thus the allele was renamed chs3-2D. CHS3 encodes a TIR-NB-LRR R protein that contains a C-terminal zinc-binding LIM (Lin-11, Isl-1, Mec-3) domain. Although this protein has been previously implicated in cold stress and defense response, the role of the LIM domain in modulating protein activity is unclear. The chs3-2D allele contains a G to A point mutation causing a C1340 to Y1340 substitution close to the LIM domain. It encodes a dominant gain-of-function mutation. The chs3-2D mutant is severely stunted and displays curled leaf morphology. Additionally, it constitutively expresses PATHOGENESIS-RELATED (PR) genes, accumulates salicylic acid, and shows enhanced resistance to the virulent oomycete isolate Hyaloperonospora arabidopsidis (H.a.) Noco2. Subcellular localization assays using GFP fusion constructs indicate that both CHS3 and chs3-2D localize to the nucleus. A third chs3 mutant allele, chs3-3D, was identified in an unrelated genetic screen in our lab. This allele contains a C to T point mutation resulting in an M1017 to V1017 substitution in the LRR–LIM linker region. Additionally, a chs3-2D suppressor screen identified two revertant alleles containing secondary mutations that abolish the mutant morphology. Analysis of the locations of these molecular lesions provides support for the hypothesis that the LIM domain represses CHS3 R-like protein activity. This repression may occur through either autoinhibition or binding of a negative defense regulator

The effect of personalized intervention on the cognitive function of elderly inpatients

目的  对住院老年患者进行早期认知功能护理干预，评价其效果。方法  2013年1月—2015年1月入院老年科病房的120名患者，运用MMSE量表对其认知功能进行评价，观察干预效果。结果  MMSE总分及MMSE量表中时间定向、短期记忆及插画条目干预后有改善（P＜0.05），地点定向、语言记忆、注意力、物品命名、语言复述、阅读能力、语言能力及语言表达条目改善不明显（P＞0.05）。 结论  个性化的认知干预能够延缓和改善患者认知功能障碍，提高老年患者的生活质量。Objectives: To assess the effect of the early cognitive function nursing intervention on the cognitive function of elderly inpatients. Methods: A total of 120 elderly inpatients in geriatrics ward were rolled from January 2013 to January 2015, and we evaluate the cognitive function of them and gave personalized intervention to them by MMSE scale. Results: The total score of MMSE was increased (P＜0.05); The entries of time orientation, short-term memory and illustration were improved significantly after three-month intervention (P＜0.05); The entries of place orientation, language, memory, attention, article name, repeat language, reading skills, language skills and language were not improved significantly. Conclusions:  Personalized cognitive intervention could improve the cognitive dysfunction and the quality of life of elderly patients

Phage display mediated immuno-PCR

Immuno-PCR (IPCR) is a powerful detection technology in immunological study and clinical diagnosis due to its ultrasensitivity. Here we introduce a new strategy termed phage display mediated immuno-PCR (PD-IPCR). Instead of utilization of monoclonal antibody (mAb) and chemically bond DNA that required in the conventional IPCR, a recombinant phage particle is applied as a ready reagent for IPCR experiment. The surface displayed single chain variable fragment (scFv) and phage DNA themselves can directly serve as detection antibody and PCR template, respectively. The aim of the design is to overcome shortcoming of low detection sensitivity of scFv so as to largely facilitate the real application of scFv in immunoassay. The idea has been demonstrated by applying hantaan virus nucleocapsid protein (NP) and prion protein (PrP) as detection targets in three experimental protocols (indirect, sandwich and real-time PD-IPCR assays). The detection sensitivity was increased 1000- to 10 000-folds compared with conventional enzyme-linked immunosorbent assays (ELISAs). This proof-of-concept study may serve as a new model to develop an easy to operate, low cost and ultrasensitive immunoassay method for broad applications