FT-IR PROFILE SCREENING OF BIOACTIVE CHEMICAL COMPONENTS IN AQUEOUS EXTRACT OF ABRUS PRECATORIUS LINN PLANT LEAF

Objective: The FT-IR profile screening is aimed to focus on the bioactive chemical components analysis of aqueous extract of Abrus precatorius Linn plant leafs. Methods: The profile screening for the bioactive chemical components analysis was performed with standard methods by using FT-IR spectral technique. Results: The aqueous extract of the leaf were screened for the various bioactive functional chemical components. The spectrum of FT-IR showed the presence different functional groups of chemical constituents such as alcohols, phenols, carboxylic acids, amide, aldehydes, ketones, alkanes, alkenes, aromatics, esters, ethers, aliphatic amines, aromatic amines, peptides, nitro compounds, sulphone, phosphonate, phosphoramide, phosphonic acid, phosphine, silane, amine oxides, aromatic substituted compounds, nitroso, sulphate ester and alkyl halides compounds, which showed 27 major characteristic bands of bioactive chemical components. Conclusion: The results confirm the fact that leaf of Abrus precatorius Linn plant possesses different bioactive functional chemical components and generated the FT-IR spectrum profile for the medicinally important plant

Phytochemicals and Antioxidant Activity Investigation of Butea monosperma Lam. Leaves Ethanol Extract

The aim of this study was to investigate phytochemicals and antioxidant activity of plant Butea monosperma Lam. leaves ethanol extract. The different extracts of this plant were reported the rich source contents of bioactive phytochemicals in the leaves and afford for various pharmacological activities. The ethanol extract of leaves was subjected to investigate phytochemicals and antioxidant activity by using DPPH in vitro system. The provided evidence of results concluded that the ethanol extract of Butea monosperma Lam. leaves are potential sources of natural bioactive phytochemicals and showed potent in vitro antioxidant activity with their IC50 value of 44.16 μg/ml. Therefore phytochemical investigation of plant leaves ethanol extract was noted various bioactive phytochemicals, which may serve as a potent source of natural antioxidants

SCREENING OF PHYTOCHEMICALS AND IN VITRO ANTIDIABETIC ACTIVITY OF BAUHINIA RACEMOSA LAM. LEAVES

Objective: In this study, the leaves of medicinal plant Bauhinia racemosa Lam. with different pharmacological activities were subjected to phytochemical screening and assessment of their in vitro inhibitory potential with porcine pancreatic α-amylase enzyme to treat and management of diabetes.Methods: Plant leaves were extracted sequentially with ethanol solvent. A modified 3,5-dinitrosalicylic acid method was adopted to screen α-amylase inhibition assay. The ethanol extract was analyzed qualitatively and gas chromatography–mass spectrometry analysis technique for the active phytoconstituents according to the standard protocols.Results: A phytochemical screening of leaves extract reveals the presence of carbohydrate, alkaloids, saponin, glycosides, steroids, tannins, flavonoids, triterpenoid, and phenolic compounds. The ethanol extract reported inhibition of α-amylase enzyme activity at IC50 value 61.72 ± 0.03 μg/mL and acarbose as a standard drug at IC50 value 28.07 ± 0.02 μg/mL.Conclusion: The results of the study indicate that B. racemosa Lam. leaves contain some of bioactive phytochemicals might to be exhibiting in vitro antidiabetic activity, which was leading to decreases the rate of starch digestion