Phytochemistry, Biological Activities, and Chemical Profiling of Berberis asiatica

This study focused on chemical profiling and assessed the total phenolic and flavonoid content as well as the antioxidant and antibacterial effect of the medicinal plant Berberis asiatica. The results revealed that this plant has high concentrations of TPC (Total phenol content) and TFC (Total flavonoid content) of 37.686 ± 2.728 mg GAE/g and 115.568 ± 8.012 mg QE/g, respectively. The DPPH free radical scavenging assay demonstrated strong inhibition, with an IC50 of 205.7 ± 5.353 μg/mL, and also showed robust antibacterial properties against Staphylococcus aureus and Klebsiella pneumoniae with a zone of inhibition (ZOI) of 14 mm and 19 mm, respectively. The extract exhibited an excellent inhibitory potential against S. aureus, and K. pneumoniae with an MIC (Minimum inhibitory concentration) of 0.39 mg/mL, and 3.125 mg/mL respectively, indicating significant inhibitory action. Furthermore, the MBC (Minimum bactericidal concentration) for both S. aureus and K. pneumoniae was found to be 6.25 mg/mL, emphasizing the extract's consistent bactericidal effectiveness against these bacteria. These findings underscore the potential utility of the methanolic extract of Berberis asiatica as a natural antibacterial agent. GC-MS analysis of hexane fraction indicates the plant is rich in secondary metabolites, specifically 2,2-dimethyl-3-pentanol, 2-methyl-2-pentanol, 2,5-dimethyl-4-hydroxy-3-hexanone, 3-hexanol, 4-methyl-2-pentanol are identified. Overall, this study highlights the importance of plant-based natural products as potential sources of antioxidants and antibacterial agents that contributes to the future drug development process

Phytochemistry, Biological Activities, and Chemical Profiling of Berberis asiatica

This study focused on chemical profiling and assessed the total phenolic and flavonoid content as well as the antioxidant and antibacterial effect of the medicinal plant Berberis asiatica. The results revealed that this plant has high concentrations of TPC (Total phenol content) and TFC (Total flavonoid content) of 37.686 ± 2.728 mg GAE/g and 115.568 ± 8.012 mg QE/g, respectively. The DPPH free radical scavenging assay demonstrated strong inhibition, with an IC50 of 205.7 ± 5.353 μg/mL, and also showed robust antibacterial properties against Staphylococcus aureus and Klebsiella pneumoniae with a zone of inhibition (ZOI) of 14 mm and 19 mm, respectively. The extract exhibited an excellent inhibitory potential against S. aureus, and K. pneumoniae with an MIC (Minimum inhibitory concentration) of 0.39 mg/mL, and 3.125 mg/mL respectively, indicating significant inhibitory action. Furthermore, the MBC (Minimum bactericidal concentration) for both S. aureus and K. pneumoniae was found to be 6.25 mg/mL, emphasizing the extract's consistent bactericidal effectiveness against these bacteria. These findings underscore the potential utility of the methanolic extract of Berberis asiatica as a natural antibacterial agent. GC-MS analysis of hexane fraction indicates the plant is rich in secondary metabolites, specifically 2,2-dimethyl-3-pentanol, 2-methyl-2-pentanol, 2,5-dimethyl-4-hydroxy-3-hexanone, 3-hexanol, 4-methyl-2-pentanol are identified. Overall, this study highlights the importance of plant-based natural products as potential sources of antioxidants and antibacterial agents that contributes to the future drug development process

Demand, End-Uses, and Conservation of Alpine Medicinal Plant Neopicrorhiza scrophulariiflora (Pennell) D. Y. Hong in Central Himalaya

Neopicrorhiza scrophulariiflora (Pennell) D. Y. Hong of Scrophulariaceae family (hereinafter referred to as Neopicrorhiza) has medicinally important rhizomes with high levels of trade. What factors drive demand for Neopicrorhiza in Central Himalaya is unknown. In this context, a nationwide comprehensive survey was conducted from September 2016 to March 2017 to assess demand, end-uses, and conservation of dry Neopicrorhiza rhizomes in Nepal. A total of 2313 herbal products were surveyed for Neopicrorhiza as an ingredient in 38 retailer shops. Processing industries of Neopicrorhiza in Nepal were interviewed using structured questionnaire. There were 23 herbal industries manufacturing 45 types of ayurvedic medicines as end-products containing Neopicrorhiza. The volume and value of annual demand for dry rhizomes of Neopicrorhiza in Nepal were found as 6076 kg and NRs 8573236 (USD 83235.30), respectively, in 2015/016 with average 264.17 kg/industry and NRs 1410.87 (USD 13.69) per kg. The major uses of ayurvedic medicines containing Neopicrorhiza were to treat a number of disease categories: cardiovascular system/liver (17), cardiovascular system/blood (6), nervous system (6), dermatological system (4), musculoskeletal system (3), digestive system (2), respiratory system (2), genitourinal system (4), and others (1). Despite changing legal regulation, trade and consumption of Neopicrorhiza exist in Nepal. It can be concluded that domestic consumption is not the major cause of resource depletion of Neopicrorhiza in Nepal

Consumers Caring Health with End Products (Ayurvedic Medicines) Containing Neopicrorhiza scrophulariiflora in Nepal

Neopicrorhiza scrophulariiflora (Pennell) D. Y. Hong is an endangered herbaceous medicinal plant found in subalpine and alpine zone of eastern Himalayas comprising Sikkim, Nepal, Bhutan and China. Rhizomes of Neopicrorhiza scrophulariiflora (hereinafter referred to as Neopicrorhiza) have medicinal properties and are highly traded in Nepal, India and China. Information on driving factors for demand of Neopicrorhiza from consumer perspective is unknown, knowledge of which is important because it can allow insights into future demand and sustainability of future trade. In this context, a survey was conducted among purchasers and consumers (patients) of end products (Ayurvedic medicines) containing Neopicrorhiza (n=513) in five different cities of Nepal from March to July 2017 using structured questionnaire to explore characteristics of consumers and consumption of end products of Neopicrorhiza. The utilization of Ayurvedic medicines containing Neopicrorhiza for treatment of health disorders of human is prevalent in Nepal. Men and women patients of diverse ages and with different socio-economic profiles were found to consume Ayurvedic medicines containing Neopicrorhiza to treat health disorders associated with different organ systems of body. The patients receiving Ayurvedic medicines containing Neopicrorhiza were children, adults and elders ranging from 1 to 98 years old (av. 37.6 years). Employed and unemployed, educated and illiterate consumers were found to consume Ayurvedic medicines containing Neopicrorhiza to treat their health disorders in Nepal. Patients receiving those medicines were from households having diverse annual cash income levels. The purchase and consumption of Ayurvedic medicine containing Neopicrorhiza was most often guided by a doctor’s prescription. Most of the respondents believed that the prices of those Ayurvedic medicines were fairer than other types of medicines. Similar studies are recommended in other countries like India and China where the Neopicrorhiza is exported from Nepal

Why do patients choose to consume Ayurvedic Medicines in Nepal? An exploratory study

Ayurvedic medicines are believed to bring harmony and balance in mind, body, spiritual well-being and social welfare of human. The reasons for preference for these medicines are of great interest for exploration. This study was carried out in Nepal in 2017 to explore the reasons for choosing to consume Ayurvedic medicines, with support of a separate survey focusing on these medicines containing Neopicrorhiza scrophulariiflora. An online survey (n=58) and face to face interviews (n=406) were administered using structured questionnaires to explore those reasons for choice. Neopicrorhiza scrophulariiflora (Pennell) D. Y. Hong (Scrophulariaceae) is a perennial alpine herb found in the subalpine as well as alpine zone of the eastern Himalayas comprising Sikkim, Nepal, Bhutan and China. Its rhizomes are medicinally important and in trade. Fewer or no side effects, preference to natural way of healing, belief regarding healing of disease, ease of access (availability and cost), and advertisement and recommendation from others were the major reasons that the patients chose to consume Ayurvedic medicines for own's healing. In case of Ayurvedic medicines containing Neopicrorhiza scrophulariiflora, majority of the patients believed that these medicines have fewer or no side effects, can cure illness particularly for long run of chronic diseases, were cheaper than allopathic medicines, were easily available to purchase and were recommended by family members. Healing was not successful in past from allopathic and other types of medicines of majority of the patients who preferred to consume Ayurvedic medicines containing Neopicrorhiza scrophulariiflora. Further scientific studies and interventions are recommended for concerned health authorities and nursing practitioners of Nepal to test and validate the safety and effectiveness of the consumption of Ayurvedic medicines. &nbsp