Hepatoprotective agent tethered isoniazid for the treatment of drug-induced hepatotoxicity: Synthesis, biochemical and histopathological evaluation

AbstractThe aim of the study was to investigate the protective effect of isoniazid–curcumin conjugate (INH–CRM) in INH-induced hepatic injury by biochemical analysis and histology examination of liver in Wistar rats. The biochemical analysis included determination of the levels of plasma cholesterol, triglycerides (TG), albumin content, and lipid peroxidation (MDA). INH–CRM administration resulted in a significant decrease in plasma cholesterol, TG, and MDA levels in the liver tissue homogenate with an elevation in albumin level indicating its hepatoprotective activity. Histology of the liver further confirmed the reduction in hepatic injury. The hepatoprotective with INH–CRM can be attributed to the antioxidant activity of curcumin. The conjugate probably stabilizes the curcumin molecule, preventing its presystemic metabolism thereby enhancing its bioavailability and therefore, its hepatoprotective activity. Thus, the novel INH–CRM has the potential to alleviate INH-induced liver toxicity in antitubercular treatment

Razvoj traktorskog mernog sistema za mapiranje prostorne promenljivosti pH zemljišta u realnom vremenu

Soil pH is an important physio-chemical property representing soil characteristics and plays an important role in plant growth. Soil pH affects crop yields, plant nutrient availability and soil micro-organism activity. For small areas, measuring the spatial variation in soil pH is feasible. However for the large areas, pH determination of the multiple soil samples by using traditional method in laboratory is very tedious and time consuming. Considering the need and scope of precision agriculture in India, a real time data logging pH measuring system was developed to determine the soil pH directly in the field at specific GPS locations by integrating soil pH sensor and a GPS receiver with a portable data logger. Soil mapping was done for observing the spatial variation in soil pH using ArcGIS. The results showed that the field had variation in soil pH and the developed soil pH measuring system was capable to measure the spatial variation in soil pH. The average soil pH measured by developed soil pH measuring system in different tillage treatments varied from 7.98 to 8.18, 7.60 to 7.94 and 8.01 to 8.21, respectively.pH zemljišta je važna karakteristika zemljišta koja igra važnu ulogu u utgoju biljaka. Zemljišni pH utiče na prinos useva, dostupnost nutrienata korenu biljaka i aktivnost mikroorganizama u zemljištu. Merenje prostornih promenljivosti pH vrednosti na manjim oblastima je izvodljivo. Ipak, za velike oblasti, određivanje pH većeg broja uzoraka zemljišta na uuobičajeni način u laboratoriji je naporno i dugotrajno. Imajući u vidu potrebe i opseg precizne poljoprivrede u Indiji razvijen je merni sistem za sakupljanje podataka u realnom vremenu, radi određivanja pH zemljišta direktno na parceli na pojedinačnim GPS lokacijama, integracijom pH senzora i GPS prijemnika sa prenosivim data logerom. Mapiranje zemljišta je vršeno radi praćenja prostorne promenljivosti pH zemljišta korišćenjem ArcGIS. Rezultati su pokazali promenljivost pH zemljišta i omogućili da se razvije merni sistem za merenje prostorne promenljivosti. Srednja pH vrednost izmerena ovim sistemom u različitim sistemima obrade zemljišta varirala je od 7.98 do 8.18, 7.60 do 7.94 i 8.01 do 8.21, redom