Aromatic l-amino acid decarboxylase : histochemical localization in rat kidney and lack of effect of dietary potassium or sodium loading on enzyme distribution

Utilizing a mono-specific antiserum produced in rabbits to hog kidney aromatic L-amino acid decarboxylase (AADC), the enzyme was localized in rat kidney by immunoperoxidase staining. AADC was located predominantly in the proximal convoluted tubules; there was also weak staining in the distal convoluted tubules and collecting ducts. An increase in dietary potassium or sodium intake produced no change in density or distribution of AADC staining in kidney. An assay of AADC enzyme activity showed no difference in cortex or medulla with chronic potassium loading. A change in distribution or activity of renal AADC does not explain the postulated dopaminergic modulation of renal function that occurs with potassium or sodium loading

Chemoenzymatic approaches to the decahydro-as-indacene cores associated with the spinosyn class of insecticide

The enantiopure dienes 8 and 24, which have been prepared by chemoenzymatic methods, engage in Diels-AIder cycloaddition reactions with maleic and citraconic anhydride to give adducts (e.g. 25-27) embodying the ABC-ring system associated with spinosyns