1,879 research outputs found
The time-dependent localization of Ki 67 antigen-positive cells in human skin wounds
A total of 77 human skin wounds with a post-infliction interval between 3 h and 7 months were investigated and the proliferation marker antigen Ki 67 was visualized in paraffin sections using a specific monoclonal antibody (MIB). The re-built epidermal layer covering the former lesional area showed only a few basal cells positively staining for Ki 67 antigen. No enhanced reactivity was found when compared to uninjured skin. In basal cells of the epidermis adjacent to the wound area, however, varying numbers of positive cells occurred, but no information useful for a reliable time estimation of skin wounds could be obtained due to the considerable variability in the number of Ki 67 positive epidermal basal cells found in non-damaged skin. Fibroblastic cells in the wound area revealed an increased number of Ki 67-positive sites which could first be detected in a 1.5-day-old skin lesion. Positive results could be obtained in every specimen investigated after a post-infliction interval of 6 days up to 1.5 months. Only the scar tissue of the oldest wound examined (wound age 7 months) revealed no increase in the number of positively staining fibroblasts. Therefore, positive results indicate a wound age of at least approximately 1.5 days and the lack of an increased number of positive fibroblastic cells in a sufficient number of specimens indicates at a wound age of less than 6 days, but cannot totally exclude longer post-infliction intervals
Stochastic optimization of a cold atom experiment using a genetic algorithm
We employ an evolutionary algorithm to automatically optimize different
stages of a cold atom experiment without human intervention. This approach
closes the loop between computer based experimental control systems and
automatic real time analysis and can be applied to a wide range of experimental
situations. The genetic algorithm quickly and reliably converges to the most
performing parameter set independent of the starting population. Especially in
many-dimensional or connected parameter spaces the automatic optimization
outperforms a manual search.Comment: 4 pages, 3 figure
Immunohistochemical localization of collagen types I and VI in human skin wounds
A total of 74 human skin wounds were investigated and collagen types I and VI were localized in the wound area by immunohistochemistry. Collagen type I appeared in the form of ramifying string-like structures after approximately 5–6 days, but positive reactions in the form of a spot-like staining around isolated fibroblasts also occurred in a skin wound aged 4 days. Collagen VI was detectable after a post-infliction interval of at least 3 days showing a strongly positive reacting network associated with fibroblasts in the wound area. Both collagens appeared almost constantly after a wound age of 6–7 clays and could also be found in wounds aged a few months. Therefore, although a positive reaction for collagen type I in the form of string-like and ramifying structures around wound fibroblasts indicates a wound age of at least 5–6 days, a spot-like positive staining for collagen type I cannot exclude a wound age of at least 4 days. A positive staining for collagen type VI represents a post-infliction time of 3 days or more. The almost constant appearance of these collagen types suggests that negative results in a sufficient number of specimens indicate a wound age of less than 6–7 days, but cannot completely exclude longer post-infliction intervals. Since collagen type I and VI are also found in the granulation/scar tissue of lesions with advanced wound age, the immunohistochemical analysis of these proteins provides no further information for an age determination of older skin wounds
Single-particle-sensitive imaging of freely propagating ultracold atoms
We present a novel imaging system for ultracold quantum gases in expansion.
After release from a confining potential, atoms fall through a sheet of
resonant excitation laser light and the emitted fluorescence photons are imaged
onto an amplified CCD camera using a high numerical aperture optical system.
The imaging system reaches an extraordinary dynamic range, not attainable with
conventional absorption imaging. We demonstrate single-atom detection for
dilute atomic clouds with high efficiency where at the same time dense
Bose-Einstein condensates can be imaged without saturation or distortion. The
spatial resolution can reach the sampling limit as given by the 8 \mu m pixel
size in object space. Pulsed operation of the detector allows for slice images,
a first step toward a 3D tomography of the measured object. The scheme can
easily be implemented for any atomic species and all optical components are
situated outside the vacuum system. As a first application we perform
thermometry on rubidium Bose-Einstein condensates created on an atom chip.Comment: 24 pages, 10 figures. v2: as publishe
Plunging Airfoil: Reynolds Number and Angle of Attack Effects
Natural flight has consistently been the wellspring of many creative minds, yet recreating the propulsive systems of natural flyers is quite hard and challenging. Regarding propulsive systems design, biomimetics offers a wide variety of solutions that can be applied at low Reynolds numbers, achieving high performance and maneuverability systems. The main goal of the current work is to computationally investigate the thrust-power intricacies while operating at different Reynolds numbers, reduced frequencies, nondimensional amplitudes, and mean angles of attack of the oscillatory motion of a NACA0012 airfoil. Simulations are performed utilizing a RANS (Reynolds Averaged Navier-Stokes) approach for a Reynolds number between 8.5×10^3 and 3.4×10^4, reduced frequencies within 1 and 5, and Strouhal numbers from 0.1 to 0.4. The influence of the mean angle-of-attack is also studied in the range of 0º to 10º. The outcomes show ideal operational conditions for the diverse Reynolds numbers, and results regarding thrust-power correlations and the influence of the mean angle-of-attack on the aerodynamic coefficients and the propulsive efficiency are widely explored.Fundação para a Ciência e a Tecnologia e Santander-UBIinfo:eu-repo/semantics/publishedVersio
Derivation of fluid dynamics from kinetic theory with the 14--moment approximation
We review the traditional derivation of the fluid-dynamical equations from
kinetic theory according to Israel and Stewart. We show that their procedure to
close the fluid-dynamical equations of motion is not unique. Their approach
contains two approximations, the first being the so-called 14-moment
approximation to truncate the single-particle distribution function. The second
consists in the choice of equations of motion for the dissipative currents.
Israel and Stewart used the second moment of the Boltzmann equation, but this
is not the only possible choice. In fact, there are infinitely many moments of
the Boltzmann equation which can serve as equations of motion for the
dissipative currents. All resulting equations of motion have the same form, but
the transport coefficients are different in each case.Comment: 15 pages, 3 figures, typos fixed and discussions added; EPJA: Topical
issue on "Relativistic Hydro- and Thermodynamics
Variations of Hadron Masses and Matter Properties in Dense Nuclear Matter
Using a self-consistent quark model for nuclear matter we investigate
variations of the masses of the non-strange vector mesons, the hyperons and the
nucleon in dense nuclear matter (up to four times the normal nuclear density).
We find that the changes in the hadron masses can be described in terms of the
value of the scalar mean-field in matter. The model is then used to calculate
the density dependence of the quark condensate in-medium, which turns out to be
well approximated by a linear function of the nuclear density. Some relations
among the hadron properties and the in-medium quark condensate are discussed.Comment: 22 pages, University of Adelaide preperint ADP-94-20/T160, submitted
to Physical Review
Simulations of energetic beam deposition: from picoseconds to seconds
We present a new method for simulating crystal growth by energetic beam
deposition. The method combines a Kinetic Monte-Carlo simulation for the
thermal surface diffusion with a small scale molecular dynamics simulation of
every single deposition event. We have implemented the method using the
effective medium theory as a model potential for the atomic interactions, and
present simulations for Ag/Ag(111) and Pt/Pt(111) for incoming energies up to
35 eV. The method is capable of following the growth of several monolayers at
realistic growth rates of 1 monolayer per second, correctly accounting for both
energy-induced atomic mobility and thermal surface diffusion. We find that the
energy influences island and step densities and can induce layer-by-layer
growth. We find an optimal energy for layer-by-layer growth (25 eV for Ag),
which correlates with where the net impact-induced downward interlayer
transport is at a maximum. A high step density is needed for energy induced
layer-by-layer growth, hence the effect dies away at increased temperatures,
where thermal surface diffusion reduces the step density. As part of the
development of the method, we present molecular dynamics simulations of single
atom-surface collisions on flat parts of the surface and near straight steps,
we identify microscopic mechanisms by which the energy influences the growth,
and we discuss the nature of the energy-induced atomic mobility
Electromagnetic Form Factors of the Nucleon in an Improved Quark Model
Nucleon electromagnetic form factors are studied in the cloudy bag model
(CBM) with center-of-mass and recoil corrections. This is the first
presentation of a full set of nucleon form factors using the CBM. The center of
mass motion is eliminated via several different momentum projection techniques
and the results are compared. It is found that the shapes of these form factors
are significantly improved with respect to the experimental data if the Lorentz
contraction of the internal structure of the baryon is also appropriately taken
into account.Comment: revtex, 28 pages, 8 ps figs include
Use of genetically modified muscle and fat grafts to repair defects in bone and cartilage
We report a novel technology for the rapid healing of large osseous and chondral defects, based upon the genetic modification of autologous skeletal muscle and fat grafts. These tissues were selected because they not only possess mesenchymal progenitor cells and scaffolding properties, but also can be biopsied, genetically modified and returned to the patient in a single operative session. First generation adenovirus vector carrying cDNA encoding human bone morphogenetic protein-2 (Ad.BMP-2) was used for gene transfer to biopsies of muscle and fat. To assess bone healing, the genetically modified ("gene activated") tissues were implanted into 5mm-long critical size, mid-diaphyseal, stabilized defects in the femora of Fischer rats. Unlike control defects, those receiving gene-activated muscle underwent rapid healing, with evidence of radiologic bridging as early as 10 days after implantation and restoration of full mechanical strength by 8 weeks. Histologic analysis suggests that the grafts rapidly differentiated into cartilage, followed by efficient endochondral ossification. Fluorescence in situ hybridization detection of Y-chromosomes following the transfer of male donor muscle into female rats demonstrated that at least some of the osteoblasts of the healed bone were derived from donor muscle. Gene activated fat also healed critical sized defects, but less quickly than muscle and with more variability. Anti-adenovirus antibodies were not detected. Pilot studies in a rabbit osteochondral defect model demonstrated the promise of this technology for healing cartilage defects. Further development of these methods should provide ways to heal bone and cartilage more expeditiously, and at lower cost, than is presently possible
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