Analysis of the Effects of Dam Release Properties and Ambient Groundwater Flow on Surface Water‐Groundwater Exchange Over a 100‐km‐Long Reach

Hydroelectric dams often create highly dynamic downstream flows that promote surface water‐groundwater (SW‐GW) interactions including bank storage, the temporary storage of river water in the riverbank. Previous research on SW‐GW exchanges in dammed rivers has primarily been at single study sites, which has limited the understanding of how these exchanges evolve as dam releases travel downstream. This study evaluates how dam releases affect SW‐GW exchange continuously over a 100‐km distance. This is accomplished by longitudinally routing water releases through a synthetic river and modeling bed and bank fluid and solute exchange across transverse transects spaced along the reach. Peak and square dam release hydrograph shapes with three magnitudes (0.5, 1.0, and 1.5 m) were considered. The effect of four ambient groundwater flow conditions (very slightly losing, neutral, and two gaining from the perspective of the river) was evaluated for each dam release scenario. Both types of dam release shapes cause SW‐GW interaction over the entire 100‐km distance, and our results show that square type releases cause bank storage exchange well beyond this distance. Strongly gaining conditions reduce the amount of exchange and allow flushing of river‐sourced solute out of the bank after the dam pulse has passed. Both neutral and losing conditions have larger fluid and solute flux into the bank and limit the amount of solute that returns to the river. Our results support that river corridors downstream of dams have increased river‐aquifer connectivity and that this enhanced connectivity can extend at least 100 km downstream

Stressabhängige Ribonukleoproteinkomplex Formation in der Kontrolle der Prothrombin Genexpression

Die 3´Endprozessierung ist neben dem Capping und Spleißen ein wichtiger posttrans-kriptioneller Genexpressionsmechanismus. Die Prothrombin (F2) mRNA ist ein Prototyp für eine Genfamilie, die ein ungewöhnliches RNA Sequenz Motiv in der 3´ untranslatierten Regi-on aufweist. Stromaufwärts des Polyadenylierungssignals befindet sich ein Sequenzelement (USE), welches die 3´Endprozessierung sequenz- und positionsanhängig stimuliert. Dabei fungieren interessanterweise einige Spleißfaktoren (hnRNPI, U2AF35 und U2AF65) als posi-tive F2 USE-Effektorproteine, indem sie den kanonischen 3´Endprozessierungsapparat an die prä-mRNA rekrutieren. Des Weiteren wurden AU-reiche elementbindende Proteine (sog. ARE-BPs) als hochaffine und spezifische F2 USE Interaktionspartner identifiziert, deren po-tentielle Bedeutung in der 3´Endprozessierung bisher nicht beschrieben und analysiert wurde. Ziel dieser Arbeit war es die Ribonukleoprotein (RNP) Komposition exemplarisch an dem F2 USE Motiv unter Stressbedingungen zu analysieren und dessen Einfluss auf die Regulation der 3´Endprozessierung zu untersuchen. In molekularen Analysen des F2 USE RNP Komplexes konnte gezeigt werden, dass die meis-ten ARE-BPs direkt und spezifisch an das USE Motiv binden. Stress führt zur Hyperphospho-rylierung von einigen der ARE-BPs (FBP2 und FBP3) und reduziert deren Bindungsaffinität zum F2 USE Motiv. Somit scheinen die FBPs als Stresssensoren zu fungieren, wodurch die Komposition des F2 USE RNP Komplexes dynamisch an physiologische Prozesse angepasst werden kann. Diese und weitere im Labor generierten Daten deuten darauf hin, dass FBP2 und FBP3 wahrscheinlich stressabhängig mit den positiven USE-Effektorproteinen um die Bindung an das F2 USE Motiv konkurrieren. Um die Frage zu beantworten, ob die 3´Endprozessierung der F2 mRNA unter Stressbedin-gungen reguliert wird, wurden stabile Reporter-Zelllinien generiert. In Stressexperimenten verhielten sich die stabilen Zellklone sehr heterogen bzgl. der Regulation der 3´Endprozessierung, was möglicherweise mit der zufälligen Integration der Tandemreporter in das Genom zu erklären ist. Trotz dieser Variabilität deuten aber diese in vivo Daten auf eine USE-abhängige Regulation der 3´Endprozessierung hin, was jedoch mit neuen experimentel-len Ansätzen wie zum Beispiel lokusspezifischen, stabil transfizierten Zelllinien zu prüfen ist. Insgesamt zeigt die hier vorgestellte Arbeit, dass die regulierte 3´Endprozessierung eine be-deutende Rolle bei der stressabhängigen Adaptation der Genexpression spielt

Active Layer Groundwater Flow: The Interrelated Effects of Stratigraphy, Thaw, and Topography

The external drivers and internal controls of groundwater flow in the thawed “active layer” above permafrost are poorly constrained because they are dynamic and spatially variable. Understanding these controls is critical because groundwater can supply solutes such as dissolved organic matter to surface water bodies. We calculated steady‐state three‐dimensional suprapermafrost groundwater flow through the active layer using measurements of aquifer geometry, saturated thickness, and hydraulic properties collected from two major landscape types over time within a first‐order Arctic watershed. The depth position and thickness of the saturated zone is the dominant control of groundwater flow variability between sites and during different times of year. The effect of water table depth on groundwater flow dwarfs the effect of thaw depth. In landscapes with low land‐surface slopes (2–4%), a combination of higher water tables and thicker, permeable peat deposits cause relatively constant groundwater flows between the early and late thawed seasons. Landscapes with larger land‐surface slopes (4–10%) have both deeper water tables and thinner peat deposits; here the commonly observed permeability decrease with depth is more pronounced than in flatter areas, and groundwater flows decrease significantly between early and late summer as the water table drops. Groundwater flows are also affected by microtopographic features that retain groundwater that could otherwise be released as the active layer deepens. The dominant sources of groundwater, and thus dissolved organic matter, are likely wet, flatter regions with thick organic layers. This finding informs fluid flow and solute transport dynamics for the present and future Arctic.Plain Language SummaryGroundwater flow in permafrost watersheds is potentially a key component of global carbon budgets because permafrost soil stores vast amounts of carbon that could be mobilized due to a warming climate and the corresponding increase in soil thaw. In addition to carrying carbon, groundwater can supply important nutrients and solutes to surface waters. However, we do not yet understand the factors that control groundwater flow in soils above permafrost because saturation changes rapidly and continuously, and soil hydraulic properties are largely unknown. We created measurement‐informed calculations of groundwater flow from areas of permafrost with different characteristics and found that soil types, which vary based on the slope of the land surface, are the most important control. Near‐surface soils were identical in hillslopes and valleys, whereas deeper soils in hillslopes allowed for less groundwater flow than in valleys. In early summer, when only the near‐surface soils were thawed, groundwater flows in the hillslopes and valley were similar. In late summer, when the deeper soil was thawed, groundwater flow in the valley remained high, but flow in the hillslope was negligible. Our observations also showed that small mounds on the land surface caused groundwater to be trapped behind underground ice dams.Key PointsDetailed measurements of hydraulic head, hydraulic conductivity, and saturated thicknesses in active layers were made over time and spaceThree main soil layers consistently comprise the stratigraphy of the active layer across the studied Arctic watershedGroundwater flow depends most on the depth of the water table and the subsurface stratigraphy, which varies based on landscape typePeer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/151862/1/wrcr24085_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/151862/2/wrcr24085.pd

Active Layer Groundwater Flow: The Interrelated Effects of Stratigraphy, Thaw, and Topography

The external drivers and internal controls of groundwater flow in the thawed “active layer” above permafrost are poorly constrained because they are dynamic and spatially variable. Understanding these controls is critical because groundwater can supply solutes such as dissolved organic matter to surface water bodies. We calculated steady‐state three‐dimensional suprapermafrost groundwater flow through the active layer using measurements of aquifer geometry, saturated thickness, and hydraulic properties collected from two major landscape types over time within a first‐order Arctic watershed. The depth position and thickness of the saturated zone is the dominant control of groundwater flow variability between sites and during different times of year. The effect of water table depth on groundwater flow dwarfs the effect of thaw depth. In landscapes with low land‐surface slopes (2–4%), a combination of higher water tables and thicker, permeable peat deposits cause relatively constant groundwater flows between the early and late thawed seasons. Landscapes with larger land‐surface slopes (4–10%) have both deeper water tables and thinner peat deposits; here the commonly observed permeability decrease with depth is more pronounced than in flatter areas, and groundwater flows decrease significantly between early and late summer as the water table drops. Groundwater flows are also affected by microtopographic features that retain groundwater that could otherwise be released as the active layer deepens. The dominant sources of groundwater, and thus dissolved organic matter, are likely wet, flatter regions with thick organic layers. This finding informs fluid flow and solute transport dynamics for the present and future Arctic

Groundwater Flow and Exchange Across the Land Surface Explain Carbon Export Patterns in Continuous Permafrost Watersheds

Groundwater flow regimes in the seasonally thawed soils in areas of continuous permafrost are relatively unknown despite their potential role in delivering water, carbon, and nutrients to streams. Using numerical groundwater flow models informed by observations from a headwater catchment in arctic Alaska, United States, we identify several mechanisms that result in substantial surface‐subsurface water exchanges across the land surface during downslope transport and create a primary control on dissolved organic carbon loading to streams and rivers. The models indicate that surface water flowing downslope has a substantial groundwater component due to rapid surface‐subsurface exchanges across a range of hydrologic states, from unsaturated to flooded. Field‐based measurements corroborate the high groundwater contributions, and river dissolved organic carbon concentrations are similar to that of groundwater across large discharge ranges. The persistence of these groundwater contributions in arctic watersheds will influence carbon export to rivers as thaw depth increases in a warmer climate

Groundwater Flow and Exchange Across the Land Surface Explain Carbon Export Patterns in Continuous Permafrost Watersheds

Groundwater flow regimes in the seasonally thawed soils in areas of continuous permafrost are relatively unknown despite their potential role in delivering water, carbon, and nutrients to streams. Using numerical groundwater flow models informed by observations from a headwater catchment in arctic Alaska, United States, we identify several mechanisms that result in substantial surface‐subsurface water exchanges across the land surface during downslope transport and create a primary control on dissolved organic carbon loading to streams and rivers. The models indicate that surface water flowing downslope has a substantial groundwater component due to rapid surface‐subsurface exchanges across a range of hydrologic states, from unsaturated to flooded. Field‐based measurements corroborate the high groundwater contributions, and river dissolved organic carbon concentrations are similar to that of groundwater across large discharge ranges. The persistence of these groundwater contributions in arctic watersheds will influence carbon export to rivers as thaw depth increases in a warmer climate

Estimating Increased Transient Water Storage With Increases in Beaver Dam Activity

Dam building by beaver (Castor spp.) slows water movement through montane valleys, increasing transient water storage and the diversity of residence times. In some cases, water storage created by beaver dam construction is correlated to changes in streamflow magnitude and timing. However, the total amount of additional surface and groundwater storage that beaver dams may create (and, thus, their maximum potential impact on streamflow) has not been contextualized in the water balance of larger river basins. We estimate the potential transient water storage increases that could be created at 5, 25, 50, and 100% of maximum modeled beaver dam capacity in the Bear River basin, USA, by adapting the height above nearest drainage (HAND) algorithm to spatially estimate surface water storage. Surface water storage estimates were combined with the MODFLOW groundwater model to estimate potential increases in groundwater storage throughout the basin. We tested four scenarios to estimate potential transient water storage increases resulting from the construction of 1179 to 34,897 beaver dams, and estimated surface water storage to range from 57.5 to 72.8 m3 per dam and groundwater storage to range from 182.2 to 313.3 m3 per dam. Overall, we estimate that beaver dam construction could increase transient water storage by up to 10.38 million m3 in the Bear River basin. We further contextualize beaver dam-related water storage increases with streamflow, reservoir, and snowpack volumes

Empirical Models for Predicting Water and Heat Flow Properties of Permafrost Soils

Warming and thawing in the Arctic are promoting biogeochemical processing and hydrologic transport in carbon‐rich permafrost and soils that transfer carbon to surface waters or the atmosphere. Hydrologic and biogeochemical impacts of thawing are challenging to predict with sparse information on arctic soil hydraulic and thermal properties. We developed empirical and statistical models of soil properties for three main strata in the shallow, seasonally thawed soils above permafrost in a study area of ~7,500 km2 in Alaska. The models show that soil vertical stratification and hydraulic properties are predictable based on vegetation cover and slope. We also show that the distinct hydraulic and thermal properties of each soil stratum can be predicted solely from bulk density. These findings fill the gap for a sparsely mapped region of the Arctic and enable regional interpolation of soil properties critical for determining future hydrologic responses and the fate of carbon in thawing permafrost

Photoswitchable Organocatalysis: Using Light To Modulate the Catalytic Activities of N-Heterocyclic Carbenes

A 4,5-dithienylimidazolium salt was found to undergo electrocyclic isomerization upon exposure to UV radiation (lambda(irr) = 313 nm) under neutral and basic conditions; subsequent exposure to visible light reversed the reaction. Under ambient light and in the presence of base, the imidazolium species catalyzed transesterifications as well as amidations in a manner similar to those of previously reported N-heterocyclic carbene precatalysts. However, upon UV irradiation to effect the aforementioned photocyclization, the rate of the transesterification reaction between vinyl acetate and allyl alcohol was significantly attenuated (k(vis/UV) = 12.5), as was the rate of the condensation of ethyl acetate with aminoethanol (k(vis/UV) = 100). The rates of these reactions were successfully toggled between fast and slow states by alternating exposure to visible and UV light, respectively, thus demonstrating a rare example of a photoswitchable catalyst that operates via photomodulation of its electronic structure

Quantifying Instream Temperature Variability in Beaded Arctic Streams

This study examines the variability of in‐pool temperatures in Imnavait Creek, a beaded arctic stream consisting of small pools connected by shallow chutes, for the purpose of predicting potential impacts of climate variations on the system. To better understand heat fate and transport through this system, the dominant heat sources and sinks creating and influencing thermal stratification within even the smallest and shallowest pools must be quantified. To do this, temperature data were collected vertically within the pool water column and surrounding bed sediments during stratified conditions. These temperature and other supporting data (e.g. instream flow, weather data, and bathymetry) were used to formulate and develop an instream temperature model that captures the site‐specific processes occurring within the pools during summer low flow conditions. The model includes advective, air–water interface, and bed conduction fluxes, simplified vertical exchange between stratified pool layers, and attenuation of shortwave radiation within the water column. We present the model formulation, data collection methods used in support of model development and population, and the resulting model calibration and validation for one of the study pools. We also provide information regarding dominant heat sources and sinks and residence times of different layers within the stratified pool. We found that the dominant heat sources vary between stratified layers and that increases in thaw depths surrounding these pools due to possible climate changes can shift stratification, mixing, and instream storage dynamics, thereby influencing the fate and transport of heat and other constituents of interest (e.g. nutrients)