Time-dependent cytokine expression in bone of experimental animals after hydroxyapatite (Hap) implantation

Proinflammatory cytokines mediate bone loss around the implants in patients with peri-implant disease. However, there is no complete data about the expression of cytokines into the bone around the implants. The aim of this work was to investigate the distribution and appearance of inflammatory cytokines and anti-inflammatory proteins in the bone of jaw of experimental rabbits in different time periods after HAp implantation. Material was obtained from 8 rabbits in lower jaw 6 and 8 months after HAp implants were placed. Tissues were processed for immunohistochemical detection of tumor necrosis factor alfa (TNFα), Interleukin 1, 6, 8, 10 (IL-1, IL-6, IL-8, IL-10) and defensin 2. Results demonstrated practically unchanged expression of IL-6 and IL-10 between both - experimental and control side 6 months after implantation, while IL-1 and IL-8 notably increased in control side. IL-1 and IL-10 expression did not change in either the experimental side nor the controle side after 8 months HAP implantation, but IL-6 and IL-8 demonstrated a decrease in the control sites. Only IL-8 was elevated with time in experimental sites, while IL-10 showed individual variations in 2 cases.publishersversionPeer reviewe

Hydroxyapatite ceramics for restoration of alveolar bone

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Calcified Algae for Tissue Engineering.

This book presents the latest advances in marine structures and related biomaterials for applications in both soft- and hard-tissue engineering, as well as controlled drug delivery

EVALUATION OF THE BIOACTIVITY PROPERTIES OF SILVER DOPED HYDROXYAPATITE DEPENDING ON THE PREPARATION METHOD

In the present study in vitro bioactivity properties of silver doped hydroxyapatite (HAp/Ag) ceramic scaffolds were evaluated. HAp/Ag powder samples with 0.3 - 2.0 wt % of silver were prepared by two different modified wet precipitation methods at 90°C temperature. The Obtained fine HAp/Ag powder was used to prepare dense and porous HAp/Ag ceramic scaffolds. The bioactivity properties of dense and porous HAp/Ag ceramics scaffolds were determined in simulated body fluid (SBF). The scanning electron microscopy (SEM) investigations showed a nucleation process of calcium containing crystals and formation of bone-like apatite layer on the surface of HAp/Ag ceramic scaffolds. SEM microphotographs showed that the formed apatite layer on the dense HAp/Ag scaffolds was thicker if compared with porous HAp/Ag ceramic scaffolds. Significant differences in the formation process of bone-like apatite layer could be attributed to the differences of scaffold porosity, surface morphology and distribution of silver particles throughout the HAp/Ag ceramic scaffolds

Strontium and strontium ranelate : Historical review of some of their functions

Publisher Copyright: © 2017 Elsevier B.V.The review covers historical and last decade's scientific literature on the biological and clinical role of strontium (Sr) and strontium ranelate (Sr RAN). It enrols the description of the main effects of Sr on supportive tissue, its proven and possible morphopathogenetical mechanisms and the interaction with the bone, and especially focuses on the Sr ability to inhibit osteoclasts and affect the programmed cell death. The main experimental and clinical experience regarding the Sr RAN influence in the treatment of osteoporosis and the search for correct doses is also highlighted. The review gives insight into the role of Sr/Sr RAN on stem cells, apoptosis, animal and clinical research.publishersversionPeer reviewe