Comparative Study of Methods for Extraction of Highly Purified Hemagglutinin from H5N1 Influenza Virus Recombinant Strain (A/Astana/6:2/2009)

ABSTRACT In the present study with the objective of the surface protein hemagglutinin production for single radial immunodiffusion reaction two methods of A/H5N1 recombinant strain A/AstanaRG/6:2/2009 highly purified hemagglutinin extraction were studied. They are ion exchange chromatography and adsorption on formalinized erythrocytes using such detergents as octyl glucoside, cetyl trimethylammonium bromide, X-100 triton. Homogeneous highly purified hemagglutinin has been obtained using the ion exchange chromatography