Characterisation of New Bacillus circulans Strain Isolated from Oil Shale

Novi soj vrste Bacillus otkriven je tijekom demineralizacije uljnoga škriljevca radi dobivanja kerogena. Učinak demineralizacije izoliranoga soja bio je kudikamo veći od učinka soja Bacillus circulans Jordan 1890. Prema biokemijskim značajkama, sastavu i strukturi proteina, sastavu masnih kiselina, kao i profilu genoma te sekvencija 16S rDNA novi je soj identificiran kao Bacillus circulans VD01.A new strain of Bacillus sp. was obtained during experiments of oil shale demineralization, which were carried out in order to get ’pure’ organic matter (kerogen). The demineralization efficiency of newly isolated strain was found to be substantially higher in comparison with that of Bacillus circulans Jordan 1890. On the basis of the biochemical characteristics, protein patterns and fatty acid composition, as well as the whole genome profile and 16S rDNA sequencing, the new strain was identified as Bacillus circulans VD01

Genetic characterization of pathogenic fluorescent pseudomonads isolated from necrotic cherry and plum buds in Serbia

During past few years a symptoms of plum and cherry bud necrosis were observed in some regions with significant cherry production in Serbia. Gram negative, fluorescent, oxidative bacterial strains were isolated from the margin of necrotic tissue. All investigated strains are levan and HR positive, while negative results are recorded in oxidase, pectinase and arginin dihydrolase tests (LOPAT+---+). Symptoms similar to those observed in natural infection were obtained after artificial inoculation of cherry leaf scares and dormant one year old cherry shoots. Investigated strains as well as reference strain of P. syringae pv. morsprunorum cause the superficial necrosis on artificially inoculated immature cherry fruits, but negative results were recorded in immature pear and lemon fruit tests as well as syringae leaves and bean pods. Gelatin and aesculin tests were negative and tyrosinase and tartrate were positive. Investigated strains isolated from necrotic cherry buds had identical REP-PCR pattern with reference strain of P. syringae pv. morsprunorum. On the basis of obtained results, it was concluded that this bacterium is causal agent of cherry trees bud necrosis in Serbia. [Projekat Ministarstva nauke Republike Srbije, br. 31018 i br. 173026

Specificity of the pathovar or ubiquity of the species: the case of Pseudomonas syringae pv. aptata

Pseudomonas syringae is complex species and the most frequently emerging group of plant pathogenic bacteria. During 2013, in Vojvodina fields 104 bacterial isolates from sugar beet that showed symptoms of bacterial leaf spot disease was gathered. Isolates were identified as Pseudomonas syringae pv. aptata using pathovar specific primers. DNA fingerprinting methods revealed high genetic variability represented with 25 groups of profiles. Even more variability was detected when PFGE and MLSA were included in the analysis, clustering the isolates in 3 and 4 major groups, respectively. Partial sequence of the citrate synthase housekeeping gene (cts) put all the analyzed isolates into 02d phylogenetic group of the P. syringae complex. Also, high diversity in pathogenicity of isolates was detected. Isolates belonging to the same group according to their aggressiveness belonged, in most cases, to the same MLST cluster. Although P. syringae pv. aptata is denominated as a pathogen specific for sugar beet, the host range analysis conducted for 20 isolates on 16 plant species showed very diverse spectrums. Moreover, two isolates exhibited the capacity to cause leaf spot disease in all tested plants. These results, combined with more extensive analysis of numerous P. syringae strains/pathovars pointed out that host range in the whole of P. syringae complex is an overlapping continuum of pathogenic potential. In addition, like most pathogenic bacteria, P. syringae is not obligatory parasitic; it has the potential to thrive in environment as a saprotroph. To study bacteria beyond plant-pathogen context we isolated Pseudomonas spp. from waters of the Sava and Danube rivers. Less than 2% of total Pseudomonas isolates were identified as P. syringae. To investigate the potential of such a scarce subpopulation of P. syringae to cause contamination of agricultural fields as well as to probe the notion that non-agricultural environment could be important reservoirs of new disease outbreaks, analysis of water from the water baring channels conecting large rivers with fields is, thus, necessary

Supplementary data for article: Dimkic, I.; Stankovic, S.; Nišavic, M.; Petkovic, M.; Ristivojevic, P.; Fira, D.; Beric, T. The Profile and Antimicrobial Activity of Bacillus Lipopeptide Extracts of Five Potential Biocontrol Strains. Frontiers in Microbiology 2017, 8 (MAY). https://doi.org/10.3389/fmicb.2017.00925

Supplementary material for: [https://doi.org/10.3389/fmicb.2017.00925]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2463

Supplementary data for the article: Ristivojević, P.; Dimkić, I.; Trifković, J.; Berić, T.; Vovk, I.; Milojkovič-Opsenica, D.; Stanković, S. Antimicrobial Activity of Serbian Propolis Evaluated by Means of MIC, HPTLC, Bioautography and Chemometrics. PLoS ONE 2016, 11 (6). https://doi.org/10.1371/journal.pone.0157097

Supplementary material for: [https://doi.org/10.1371/journal.pone.0157097]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2261

Supplementary data for the article: Dimkić, I.; Ristivojević, P.; Janakiev, T.; Berić, T.; Trifković, J.; Milojković-Opsenica, D.; Stanković, S. Phenolic Profiles and Antimicrobial Activity of Various Plant Resins as Potential Botanical Sources of Serbian Propolis. Industrial Crops and Products 2016, 94, 856–871. https://doi.org/10.1016/j.indcrop.2016.09.065

Supplementary material for: [https://doi.org/10.1016/j.indcrop.2016.09.065]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2345

Genetic diversity of Serbian isolates of Xanthomonas campestris pv. campestris originated from winter oilseed rape

Oilseed rape (Brassica napus L.) is a hybrid species within Brassicaceae family, significant for its oil-rich seed, mainly used for vegetable oil and biodiesel production, but also for consumption as livestock feed. Yield of oilseed rape and other cruciferous crops can be reduced due to different plant pathogenic bacteria, fungi and pests. One of the most significant bacteria affecting crucifers is Xanthomonas campestris pv. campestris (Xcc) causing black rot disease. Xcc isolates from diseased winter oilseed rape plants showing bacterial blight symptom on leaves were collected in nine-year period (2010-2018), from different localities in Serbia, with the aim to examine their genetic features, through sequencing of housekeeping genes. DNA of the obtained isolates was therefore amplified with six primers (dnaK, fyuA, gapA, gyrB, lepA, rpoD) and sent for sequencing. The obtained sequences were checked for homology with strains available in NCBI database

GENETIC DIVERSITY OF SERBIAN ISOLATES OF XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ORIGINATED FROM WINTER OILSEED RAPE

Oilseed rape (Brassica napus L.) is a hybrid species within Brassicaceae family, significant for its oil-rich seed, mainly used for vegetable oil and biodiesel production, but also for consumption as livestock feed. Yield of oilseed rape and other cruciferous crops can be reduced due to different plant pathogenic bacteria, fungi and pests. One of the most significant bacteria affecting crucifers is Xanthomonas campestris pv. campestris (Xcc) causing black rot disease. Xcc isolates from diseased winter oilseed rape plants showing bacterial blight symptom on leaves were collected in nine-year period (2010-2018), from different localities in Serbia, with the aim to examine their genetic features, through sequencing of housekeeping genes. DNA of the obtained isolates was therefore amplified with six primers (dnaK, fyuA, gapA, gyrB, lepA, rpoD) and sent for sequencing. The obtained sequences were checked for homology with strains available in NCBI database. Multilocus sequence analysis (MLSA) was then performed to determine relatedness among the tested isolates. Based on six genes, tested isolates were identified as Xcc using NCBI BLAST, showing 99-100% homology with the available data. Winter oilseed rape Xcc isolates were divided into five groups on Neighbour-joining tree, revealing intrapathovar diversity within isolates from this host. These results could be connected with pathogen adaptation to winter oilseed rape as a new host, providing completely distinct ecological niche from B. oleracea vegetable crops (broccoli, cabbage, cauliflower, etc.) which are common hosts for this bacteria. This observation on Serbian Xcc isolates gives a significant input on genetic variation and constitutes a highly informative sample of X. campestris diversity