538 research outputs found
A SspI PCR-RFLP detecting a silent allele at the goat CSN2 locus
The comparison between the cDNA sequence obtained
and the published sequences of the goat CSN2 alleles
showed a new single nucleotide polymorphism (SNP)
(transition C-T) at the 180th nucleotide of the ninth exon.
This mutation, which took place at 124 nt from the
polyadenylation site, identifies a silent allele at the CSN2
locus named CSN2 A1.
Since the 9th exon C-T transition creates a SspI
endonuclease restriction site, the SspI digestion
of a PCR product of 360 bp spanning the 9th exon and
flanking regions, would allow carriers for the presence of
thymine to be identified.
The allelic frequency of the CSN2 A1 allele, determined
in 170 goats belonging to an undefined genetic type reared
in the province of Naples (Italy), was 0.23
It has been observed that the sequences
in the 3â untranslated regions (UTR), proximal to the
polyadenylation site, can affect the mechanism of mRNA
deadenylation and degradation. Therefore, it is reasonable
to hypothesize that the C-T transition might, directly or
indirectly, influence the stability of the mRNA and,
consequently, the amount of protein produced
Detailed description of RBA-banded chromosomes of river buffalo (Bubalus bubalis L.)
International audienc
A point mutation in the splice donor site of intron 7 in the as2-casein encoding gene of the Mediterranean River buffalo results in an allele-specific exon skipping
The CSN1S2 cDNA of 10 unrelated Mediterranean
River Buffaloes reared in Southern Italy was amplified
by RT-PCR, while the region from the 6th to the 8th exon
of the CSN1S2 gene was amplified from genomic template.
cDNA sequence comparisons showed
that five individuals had a normal transcript only (named CSN1S2A), one had a
deleted transcript only (named CSN1S2B), because of the splicing out of the 27-bp of
exon 7, and the remaining four had a heterozygous pattern.
Analysis of the genomic sequences revealed a FM865620:
g.773G>C transversion that caused inactivation of the intron 7
splice donor site and, consequently, the allele-specific exon skipping
characteristic of the CSN1S2B allele. The g.773G>C
mutation creates a new AluI restriction site enabling a PCRâ
RFLP rapid genotyping assay. The cDNA sequences showed three additional
exonic mutations forming an extended haplotype with
the g.773G>C polymorphism: FM865618: c.459C>T,
c.484A>T and c.568A>G homozygous and heterozygous
respectively in the CSN1S2BB and CSN1S2AB buffaloes. The
first is silent, while the remaining two are non-conservative
(p.Ile162Phe and p.Thp200Ala respectively). The genotype frequencies (37 CSN1S2A/A,
15 CSN1S2A/B and one CSN1S2B/B) are in agreement with
HardyâWeinberg equilibrium, with the
frequency of the deleted B allele being 0.16.
The predicted bubaline as2B protein
is 198 aa long instead of 207 aa and would also be characterized
by the presence of Phe at position 147 and Ala at 185
Anaerobic digestion of a fish processing industry sludge
Due to the fact that all fish processing plants operating in Portugal send their sludge from the wastewater treatment process to landfill, and because it is a costly management policy, the aim of this work was to assess the potential use of this substrate for the production of biogas through the anaerobic process technology. Anaerobic digestion has long proven to be an efficient way for the production of a renewable fuel â Biogas - that can be used as a source of energy to produce electricity and heat. This renewable energy resource can be used to reduce the plant processing costs, reducing also the industry carbon footprint. So, the produced sludge becomes a valuable sub-product of the wastewater treatment process instead of being considered a waste, with disposal costs associated. In this study we performed mesophilic (35 ± 1°C) batch assays during 51 days. The inoculum used was from an anaerobic digester operating in a municipal WWTP, also at mesophilic conditions. The anaerobic digestion of the fish sludge produced 700 m3 of CH4/ton SV, which is a really promising result
Biogas production from mediterranean crop silages
Anaerobic digestion has proven to be an efficient way for the production of a renewable fuel. The aim of this work was to study the potential use of two crop silages, yellow lupine (Lupinus luteus L.) and oilseed radish (Raphanus sativus var. oleifera cv. Pegletta), for the production of biogas through the process of anaerobic digestion. The use of yellow lupine was due to its capacity for nitrogen fixation, reducing the fertilization needs for the succeeding crop cycle and reducing also the GHG emissions due to the fertilizer production and its field application. The utilization of the oilseed radish was due to its root exudates with nematicide effect, reducing the needs for soil disinfection, working as a biological weapon and also due to the effect on soil compaction of its large roots, working as a bio-driller. The yellow lupine gave rise to 400 m3 of CH4.t-1 VS and the oilseed radish silage produced approximately 300 m3 of CH4.t-1 VS, proving to be good anaerobic substrates. The inoculum used for the batch digesters was sludge from an anaerobic digester of a WWTP
Uncovering the potential of blockchain in the agri-food supply chain: An interdisciplinary case study
Abstract BOOK SIAART
INTRODUCTION. Dental injury occurres in 0.06 - 0.13 % of general anesthesia procedures re- quiring endotracheal intubation, and it is still a reason of complaint against anesthetists. Maxil- lary central incisors are the most commonly teeth injured.1
In our center claims related to teeth damage after intubation ended with a total reimbursement of 13.000 Euros (2014-2015) and 5 patients (2014 to June 2016) were admitted for cares in Prosthodontics Implant dentistry dept. Dental School - Turin.
BUCX et al.2 used a strain gauge based sensor between handle and blade of the laryngoscope to measure forces applied on the maxillary incisors to show that during routine laryngoscopy great forces are exerted on the maxillary incisor teeth with no differences based on the operator experience.
OBJECTIVES.The aim of this study is to measure of the forces applied on teeth using a direct laryngoscope or a McGRATHÂź videolaringoscope. Many authors described how lower force on soft tissue could be applied using a VLS (GlideScope) instead of a standard laryngoscope (P=0.05).3, but no studies have ever been performed involving the use of a force sensor directly applied on teeth.
The aim of our study is to measure the forces applied on teeth after the application of a cus- tomized bite (2 mm dental bite) applied on the manikin. Anesthesiologists and anesthesia residents from âCittĂ della Salute e della Scienzaâ,Turin will be asked to perform 3 intubations using the standard intubation system, and other 3 using McGRATHÂź videolaringoscope in order to test the intubation forces exerted
A SNP in the goat CSN2 promoter region is associated with the absence of β-casein in the milk
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