Biological activities (antioxidant and antimicrobial activity) of the aqueous extracts and essential oil of Ammoides verticillata (Nounkha)

The phytochemical review conducted on the aerial part Ammoides verticillata has revealed the presence of six chemical families: flavonoids, tannins; gallic tannins, anthocyanins and the coumarins, cateichic tannins. The aqueous extract of the Ammoides verticillata could bring back the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) to the yellow-colored diphenylpicrylhydrazine with an IC50 of 0.020 mg.mL-1. It is gifted with antioxidant activity, however it was less effective than vitamin C (0.001 mL-1). The essential oil of the Ammoides verticillata has presented a strong antimicrobial activity against Gram negatif germs targets of original clinical action: E. coli (ESBL) (37 mm), E. coli (39.5 mm), Klebsiella pneumoniae (36.5 mm) and especially with the yeast: Candida albicans ATCC 10231 (47 mm) and Saccharomyces cerevisie ATCC (42 mm), which are highly sensitive to the oil inhibitory action. However, the oil has presented a low activity against the bacteria P. aeruginosa (12.5 mm)

Study of the Biological Activities of the Seeds of the Plant Ceratonia Siliqua L. Recovered in the Bejaia Region

Background: Ceratonia siliqua is a plant that belongs to the Fabaceae family. It is frequently used in our culinary and medical traditions to fight cholesterol, acute diarrhea and digestive disorders. Methods: The hydrogen atom or electron donation abilities of the corresponding extracts and some pure compounds were measured from the bleaching of the purple-colored methanol solution of 2, 20-diphenylpicrylhydrazyl (DPPH), studying the anti-inflammatory effect by measuring the volume of the edema of the paw that has received carrageenan 1%. Antimicrobial activity in vitro was screened by using disc diffusion and micro-dilution methods. Results: The determination of the polyphenols of the methanolic extracts of the seeds reveals the richness of our extracts in polyphenols is 30 mg of gallic acid / g of extract. The aqueous and methanolic extract flavonoid assay shows that they contain high levels of flavonoids with contents of 07 and 10 mg equivalent of quercetin / g of extract respectively. The aqueous and methanolic extracts of Ceratonia siliqua seeds from the Bejaia region could bring the stable free radical 2.2 diphenyl-1-picrylhydrazyl (DPPH) to yellow-colored diphenylpicrylhydrazine with respective IC 50 values of 0.7 mg/ml and 0.2 mg/ml. They exhibit lower antioxidant activity than ascorbic acid (0.038 μg / ml). Determination of anti-inflammatory activity revealed that our aqueous and methanolic extracts of Ceratonia siliqua L seeds are able to reduce edema up to 81.89%. The methanoid extracts of Ceratonia siliqua L. seeds have a medium antibacterial action against bacteria: E. coli (13 mm), Staphylococcus aureus (12 mm) and Pseudomonas aeruginosa (12 mm). On the other hand, the aqueous extracts of the seeds of Ceratonia siliqua L have a weak antibacterial action against the same bacteria with a diameter of inhibition of 9 mm. However, the aqueous extracts of Ceratonia siliqua L seeds are endowed with a very important inhibitory action against candida albicans and it is similar to that of the antibiotic, antifungal Econazole (1%). Conclusion: According to the results observed, the two extracts of the seeds and pods have a potential anti-free radical and antioxidant measured compared to the standard antioxidant used. The results of the antimicrobial activity carried out in vitro on the 3 bacterial strains indicate that the two methanolic extracts of this plant have low antibacterial activity

Study of the Anti-Inflammatory and Healing Properties of the Rhizomes of Carthamus Caeruleus L. (Asteraceae) Harvested in the Region of Tipaza

Background: In some regions of Algeria, in Tipaza, natural medicine still occupies a place of choice in the treatment of many pathologies, among these natural remedies are quoted extracts of the rhizomes of Carthamus caeruleus L are used to treat burns with these astringent properties. Medicinal plant extracts contain a variety of phenolic compounds that are attributed to various biological activities (antioxidant and antimicrobial activity). Methods: Our present study was conducted on the roots of Carthamus caeruleus L. harvested in the Tipaza region during the spring season. In order to promote this plant, we evaluated the anti-inflammatory activity and healing activity of the aqueous extract of the powder of the roots of Carthamus caereulus L. Studying the anti-inflammatory effect by measuring the volume of the edema of the paw that has received carrageenan 1%. Circular incision of 2 cm in diameter was made in Wistar rats to evaluate the healing activity of the aqueous pasty extract of the rhizomes of Carthamus caeruleus L at 100 mg/kg. Results: They show that aqueous extracts of Carthamus caeruleus L rhizomes at the dose of 100 mg/kg are opposed to the increased edema induced by the carrageenan 1% in rats with a percentage of inhibition of edema volume of 87.34% at the 6th hour.  Concerning the healing activity, the results show that the application of the aqueous extracts of the roots of Carthamus caereulus L.at dose of 100 mg/kg on the wounds causes their healing at the end of 14 days. Conclusion: Aqueous extracts of the roots of Carthamus caereulus L. show healing properties and anti-inflammatory effects. These results could justify the use of this plant in traditional medicine against inflammatory diseases

Hemisinteza derivata tiona i tiola iz eteričnog ulja biljke Artemisia judaica L. i antimikrobni testovi

The applications of organosulfur compounds, especially thioketones are varied which concerns both synthetic and biological chemistry. In this context, from the essential oil of Artemisia judaica L., extracted by hydrodistillation, and consisting mainly of piperitone (91.77 %), thione, and thiol derivatives were hemi-synthesized via a thionylation reaction byphosphorus pentasulphide (P2S5). The possible thione-thiol tautomerism in solution followed spectrophotometrically revealed the predominance of thiol form in solution. The antimicrobial activity of essential oils showed that the thionylation of essential oil caused a considerable increase in antimicrobial activity in particular against Escherichia coli. This work is licensed under a Creative Commons Attribution 4.0 International License.Iz eteričnog ulja biljke Artemisia judaica L., koje se uglavnom sastoji od piperitona (91,77 %), a dobiveno je hidrodestilacijom, reakcijom tionilacije pomoću fosforova pentasulfida (P2S5) hemisintetizirani su tion i tiol derivati. Moguća tion-tiolna tautomerija praćena je spektrofotometrijski. Analiza je pokazala da u otopini dominira tiolni oblika. Također, pokazalo se da tionilacija eteričnog ulja uzrokuje znatno povećanje antimikrobne aktivnosti, osobito kad je u pitanju bakterija Escherichia coli. Ovo djelo je dano na korištenje pod licencom Creative Commons Imenovanje 4.0 međunarodna