3 research outputs found
Human cystic echinococcosis in South Africa
Cystic echinococcosis (CE) is caused by the tapeworm, Echinococcus granulosus. The tapeworms resides in the small intestines of canids and the lifecycle involves both intermediate and definitive hosts. Humans are accidental intermediate hosts. Cystic echinococcosis is an economically important infection constituting a threat to public health, and is considered an emerging disease around the world. There are at least 10 Echinococcus strain types (G1 – G10), each exhibiting diversity of morphology, development and host range. The epidemiology of CE is poorly understood in South Africa. A retrospective data analysis of the National Health Laboratory Service (NHLS) laboratory information system on echinococcosis serology, microscopy and histopathology results in eight provinces (excluding KwaZula-Natal) showed an overall positivity rate in submitted diagnostic samples of 17.0% (1056/6211), with the Eastern Cape (30.4%), North West (19.0%) and Northern Cape (18.0%) provinces showing highest rates. The data showed considerable variability between provinces. The review also showed that most proven cases were negative on serology, implying that the actual number of patients could be underestimated. To our knowledge, no data exist about the prevalent strains of E. granulosus and this prospective study will attempt to fill that gap. The aim is to genotype strains causing the disease in South Africa. Two different polymerase chain reaction (PCR) methods will be used to respectively target the 12S rRNA and nad 1 genes. To date, three samples have been genotyped as G1, G5 and G6; suggesting diversity of strains prevalent in the country, but more data is needed for a clearer picture
Human cystic echinococcosis in South Africa
Dissertation submitted to the Faculty of Health Sciences, University of the
Witwatersrand, Johannesburg, in fulfilment of the requirements for the degree of
Master of Science in Medicine.
Johannesburg, May 2013Cystic echinococcosis is a neglected parasitic zoonosis that is regarded as an emerging
disease worldwide. Effective control of the disease is based on understanding the variability
of Echinococcus granulosus (sensu lato), as genotypic characteristics may influence lifecycle
patterns, development rate, and transmission. No molecular epidemiological research has
previously been conducted to shed light on genotypes responsible for the disease in South
Africa. To identify strains circulating in the country, parasite material was collected from
patients between August 2010 and September 2012 and analyzed by PCR/RFLP methods. A
total of 32 samples was characterized as E. granulosus (G1) (81%), E. canadensis (G6/7)
(16%) and E. ortleppi (G5) (3%). Furthermore, two co-amplifying G6/7 genotypes were
confirmed as G7 by sequencing. This is the first report on genotyping of Echinococcus
species in South Africa, and, to our knowledge, the first report of the G5 and G7 genotypes
from humans in Africa
First insights into species and genotypes of Echinococcus in South Africa
Cystic echinococcosis is a serious and neglected parasitic zoonosis that is regarded as an emerging disease world-wide. Effective control of the disease is based on understanding the variability of Echinococcus granulosus (sensu lato), as genotypic characteristics may influence lifecycle patterns, development rate, and transmission. No molecular epidemiological research has previously been conducted to shed light on genotypes responsible for the disease in South Africa. To identify strains circulating in the country, parasite material was collected from patients between August 2010 and September 2012 and analyzed by PCR/RFLP methods. A total of 32 samples was characterized as E. granulosus sensu stricto (G1-G3) (81%), E. canadensis (G6/7) (16%) and E. ortleppi (G5) (3%). Furthermore, two co-amplifying G6/7 genotypes were confirmed as G7 by sequencing. This is the first report on genotyping of Echinococcus species in South Africa, and, to the best of our knowledge, the first report of the G5 and G7 genotypes from humans in Africa. (c) 2013 Elsevier B.V. All rights reserved