PROBING BUFFER-GAS COOLED MOLECULES WITH DIRECT FREQUENCY COMB SPECTROSCOPY IN THE MID-INFRRARED

We present the first demonstration of cavity-enhanced direct frequency comb spectroscopyfootnote{A. Foltynowicz emph{et al}. Cavity-enhanced optical frequency comb spectroscopy in the mid-infrared application to trace detection of hydrogen peroxide. Applied Physics B, vol. 110, pp. 163�175, 2013.} on buffer-gas cooled moleculesfootnote{D. Patterson and J. M. Doyle. Cooling molecules in a cell for FTMW spectroscopy. Molecular Physics 110, 1757�1766, 2012.}. By coupling a mid-infrared frequency comb to a high-finesse cavity surrounding a helium buffer-gas chamber, we can gather rotationally resolved absorption spectra with high sensitivity over a broad wavelength region. The measured $sim$10 K rotational and translational temperatures of buffer-gas cooled molecules drastically simplify the observed spectra, compared to those of room temperature molecules, and allow for high spectral resolution limited only by Doppler broadening (10-100 MHz). Our system allows for the extension of high-resolution spectroscopy to larger molecules, enabling detailed analysis of molecular structure and dynamics, while taking full advantage of the powerful optical properties of frequency combs

A cryogenic beam of refractory, chemically reactive molecules with expansion cooling

Cryogenically cooled buffer gas beam sources of the molecule thorium monoxide (ThO) are optimized and characterized. Both helium and neon buffer gas sources are shown to produce ThO beams with high flux, low divergence, low forward velocity, and cold internal temperature for a variety of stagnation densities and nozzle diameters. The beam operates with a buffer gas stagnation density of ~10^15-10^16 cm^-3 (Reynolds number ~1-100), resulting in expansion cooling of the internal temperature of the ThO to as low as 2 K. For the neon (helium) based source, this represents cooling by a factor of about 10 (2) from the initial nozzle temperature of about 20 K (4 K). These sources deliver ~10^11 ThO molecules in a single quantum state within a 1-3 ms long pulse at 10 Hz repetition rate. Under conditions optimized for a future precision spectroscopy application [A C Vutha et al 2010 J. Phys. B: At. Mol. Opt. Phys. 43 074007], the neon-based beam has the following characteristics: forward velocity of 170 m/s, internal temperature of 3.4 K, and brightness of 3x10^11 ground state molecules per steradian per pulse. Compared to typical supersonic sources, the relatively low stagnation density of this source, and the fact that the cooling mechanism relies only on collisions with an inert buffer gas, make it widely applicable to many atomic and molecular species, including those which are chemically reactive, such as ThO

Advanced cold molecule electron EDM

Measurement of a non-zero electric dipole moment (EDM) of the electron within a few orders of magnitude of the current best limit of |d_e| < 1.05 × 10^(−27) e⋅cm [1] would be an indication of physics beyond the Standard Model. The ACME Collaboration is searching for an electron EDM by performing a precision measurement of electron spin precession in the metastable H^3Δ_1 state of thorium monoxide (ThO) using a slow, cryogenic beam. We discuss the current status of the experiment. Based on a data set acquired from 14 hours of running time over a period of 2 days, we have achieved a 1-sigma statistical uncertainty of δd_e = 1 × 10^(−28) e⋅cm/√T, where T is the running time in days

Advanced cold molecule electron EDM

Measurement of a non-zero electric dipole moment (EDM) of the electron within a few orders of magnitude of the current best limit of |de| < 1.05×10−27 e · cm would be an indication of physics beyond the Standard Model. The ACME Collaboration is searching for an electron EDM by performing a precision measurement of electron spin precession in the metastable H 3Delta_1 state of thorium monoxide (ThO) using a slow, cryogenic beam. We discuss the current status of the experiment. Based on a data set acquired from 14 hours of running time over a period of 2 days, we have achieved a 1-sigma statistical uncertainty of d(de) = 1×10^−28 e · cm/√T ,where T is the running time in days.Physic

STING-dependent type I IFN production inhibits cell-mediated immunity to Listeria monocytogenes.

Infection with Listeria monocytogenes strains that enter the host cell cytosol leads to a robust cytotoxic T cell response resulting in long-lived cell-mediated immunity (CMI). Upon entry into the cytosol, L. monocytogenes secretes cyclic diadenosine monophosphate (c-di-AMP) which activates the innate immune sensor STING leading to the expression of IFN-β and co-regulated genes. In this study, we examined the role of STING in the development of protective CMI to L. monocytogenes. Mice deficient for STING or its downstream effector IRF3 restricted a secondary lethal challenge with L. monocytogenes and exhibited enhanced immunity that was MyD88-independent. Conversely, enhancing STING activation during immunization by co-administration of c-di-AMP or by infection with a L. monocytogenes mutant that secretes elevated levels of c-di-AMP resulted in decreased protective immunity that was largely dependent on the type I interferon receptor. These data suggest that L. monocytogenes activation of STING downregulates CMI by induction of type I interferon