The effect of oral administration of tauroside Sx1 on the accumulation of influenza virus and histological changes in the lungs of mice

The constant threat of a new viral pandemic gives special urgency to the search for new effective means of preventing and treating influenza infection. The article examines the effect of oral administration of saponin tauroside Sx1, obtained from the leaves of Crimean ivy, on the development of infection caused by influenza virus A/WSN/1/33(H1N1), and histological changes in the lungs of infected mice. It was revealed that oral administration of saponin tauroside Sx1 at a dose of 200 mcg/mouse day or 11.8 mg/kg/day for three days after infection led to an almost twofold statistically significant increase in the average life expectancy of infected animals from 6.50±0.67 to 11.10±2.19 days. The protective activity of tauroside Sx1 was established when administered orally in the early stages of influenza infection in mice. The protective effect of saponin is manifested in a significant increase in the average life expectancy and normalization of the structure of lung tissue in infected animals. The results obtained indicate the prospects for further study of saponin tauroside Sx1 as a potential component of anti-influenza drugs

Effect of different zinc concentrations on the growth functions of spring wheat seedlings

In laboratory experiments, the effect of different concentrations of zinc on the growth processes of seedlings of spring wheat cv. Ester was studied. The optimal concentration of zinc for seed treatment before sowing was revealed. The optimal concentration of zinc has a positive effect on the length of sprouts and roots, as well as the photosynthesis of seedlings of spring wheat in the early stages of development

Properties, processes and regimes of soil solutions and surface waters

Soil solutions and the surface waters are characterized by properties, processes and regimes. Soil solutions of different soils and their surface water have different biological activity and change the activity of dissolved stimulants and inhibitors. The object of the study are soil solutions of the main types of soils obtained in the model experiments with the ratio of soils: water equal to 1:1 and 1: 2, soil solutions and surface water in the flooding of soils with water for 1 week – 3 months. The research method consisted in the assessment of pH, Eh, activity of K, NO3, NH4, Ca, Mg by conventional methods, assessment of concentrations of water-soluble compounds extracted from soils by ionite membranes, in the assessment of biological activity of solutions using biotests. The following is suggested for additional evaluation: the using of cation and anion membranes, determination of interrelation between the properties of waters, equation of pair correlation and multiply regression. The informative value of the gradient of surface water concentrations at different distances from the floor of the reservoir, at different depths of the overwatered soils is shown. The mobility of Ca, Mg, Fe, Mn in soils and the content of their water-soluble forms depends on both pH and Eh, whose influence on the content of water-soluble forms of the considered cations shows the effects of synergy and antagonism. The rate of change in the composition of soil solutions during soil flooding depends on a combination of soil properties, temperature, and duration of flooding. Soil solutions of different soils and their surface waters have differentrates

Properties, processes and modes characterizing the state of heavy metals in soils

The study proves the expediency of assessing the state of heavy metals in soils by the fractional composition of soil compounds, processes and modes. An informational and energy analysis of the state of heavy metals in soils was carried out. The need for an assessment of the kinetics of their desorption from soils was substantiated. The CH3 COONH4 solution displaced 0.3 mg/l Mn from the sod-podzolic soil within 10 minutes, 0.8 mg/l Mn – within 1 hour, 3.3 mg/l Mn – within 1 day, and 12.9 mg/l – within one week; 0.18 mg/L Pb was desorbed within 10 minutes, up to 0.59 mg/l Pb was desorbed within one day. The depositing capacity of soils is an important parameter. Heavy metals in soils are bound into positively and negatively charged systems at рКН of 2-10. In the sod-podzolic soil, the content of NiLn+ was 13.3 mg/l and NiLn-- 1.9 mg/l. According to the informational assessment, heavy metals in soils are characterized by the relationship with other soil properties and change along the soil profile, which was determined from the equations of pair correlation and multiple regression. According to the energy assessment, heavy metals in soils are characterized by thermal effects of their sorption and desorption and have positively and negatively charged complex compounds and air ion

The effect of oral administration of tauroside Sx1 on the accumulation of influenza virus and histological changes in the lungs of mice

The constant threat of a new viral pandemic gives special urgency to the search for new effective means of preventing and treating influenza infection. The article examines the effect of oral administration of saponin tauroside Sx1, obtained from the leaves of Crimean ivy, on the development of infection caused by influenza virus A/WSN/1/33(H1N1), and histological changes in the lungs of infected mice. It was revealed that oral administration of saponin tauroside Sx1 at a dose of 200 mcg/mouse day or 11.8 mg/kg/day for three days after infection led to an almost twofold statistically significant increase in the average life expectancy of infected animals from 6.50±0.67 to 11.10±2.19 days. The protective activity of tauroside Sx1 was established when administered orally in the early stages of influenza infection in mice. The protective effect of saponin is manifested in a significant increase in the average life expectancy and normalization of the structure of lung tissue in infected animals. The results obtained indicate the prospects for further study of saponin tauroside Sx1 as a potential component of anti-influenza drugs

Peroxidase activity in germinating barley grains depending on grain treatment with phytoregulators

The article presents the data of laboratory results on the study of the effect of phytoregulators on changes in the activity of peroxidase enzyme isoforms in dry seeds of malting barley and during their germination. It was found that the use of phytoregulators in dry grains increases the activity of peroxidase isoforms

Bacterial Communities of <i>Lamiacea</i> L. Medicinal Plants: Structural Features and Rhizosphere Effect

Bacterial communities associated with medicinal plants are an essential part of ecosystems. The rhizosphere effect is rather important in the cultivation process. The purpose of the study was to analyze the rhizosphere effect of oregano (Origanum vulgare L.), peppermint (Mentha piperita L.), thyme (Thymus vulgaris L.), creeping thyme (Thymus serpillum L.) and sage (Salvia officinalis L.). To estimate the quantity of 16S bacteria ribosomal genes, qPCR assays were used. To compare bacterial communities’ structure of medicinal plants rhizosphere with bulk soil high-throughput sequencing of the 16S rRNA targeting variable regions V3–V4 of bacteria was carried out. The highest bacterial abundance was associated with T. vulgaris L., M. piperita L. and S. officinalis L., and the lowest was associated with the O. vulgare L. rhizosphere. Phylum Actinobacteriota was predominant in all rhizosphere samples. The maximum bacterial α-diversity was found in S. officinalis L. rhizosphere. According to bacterial β-diversity calculated by the Bray–Curtis metric, T. vulgaris L. root zone significantly differed from bulk soil. The rhizosphere effect was positive to the Myxococcota, Bacteroidota, Verrucomicrobiota, Proteobacteria and Gemmatimonadota