Citrus Production, Constraints and Management Practices in Ethiopia: The Case of Pseudocercospora Leaf and Fruit Spot Disease

Citrus is economically important fruit crop in Ethiopia. However, its production is seriously constrained by various diseases including Pseudocercospora leaf and fruit spot. Surveys were conducted between June 2012 and May 2013 in the main citrus production areas of the country to assess the spread of the disease, and to determine its incidence and severity. A total of forty-nine citrus orchards in twenty-eight districts were surveyed. Random sampling techniques were used for data collection. The results showed that the disease had prevailed and widely spread in the districts assessed in the wet humid areas of the south, southwest, northwest, and north central parts, but not in the low altitude drier areas of the central rift valley and the eastern region of the country. Accordingly, 63.3% of the orchards surveyed were infected with the disease. The overall mean incidences of the disease on leaves of sweet orange, mandarin, lemon and lime were 36.2, 21.5, 17.1 and 16.3% while disease severity ratings were 2.6, 2.3, 2.0 and 1.7, respectively. Similarly, the average incidences and severities on fruits were 63.8, 29.4, 18.0 and 16.7%, and 4.0, 3.0, 2.0 and 2.0, in the same order. However, disease incidences in the different orchards ranged from zero to 76.7% on leaves and from zero to 100% on fruits. Disease severity ratings also varied from one to five on both leaves and fruits. In general, citrus orchards in the south and southwest Ethiopia that are known to have high rainfall and humidity conditions were more severely affected by the disease than those in the northwest and north central parts of the country. In these areas, respondents indicated that many farmers completely abandoned their citrus trees due to this disease. The causative agent was also identified to be the fungus Pseudocercospora angolensis based on cultural and morphological characteristics, and pathogenicity tests of representative isolates obtained from infected leaf and fruit samples.Keywords: Citrus Leaf and fruit spot disease; Pseudocercospora angolensis; Ethiopi

Monitoring Building Systems for Schedule Compliance

As Pacific Northwest National Laboratory (PNNL) initiated a Core Business Hours program, it became a challenge to ensure that the hundreds of systems campus wide were operating within their programmed schedules. Therefore, a collaborative exchange between PNNL operations and PNNL researchers developing the Decision Support for Operations and Maintenance (DSOM) software package was initiated to create a tool to solve this problem. This new DSOM tool verifies systems are operating within scheduled operation times by polling Building Automation and Control Network (BACnet) identifiers of systems’ on/off or command statuses. The tool records the time spent in operation state (ON) and totalizes each system over a rolling 7-day period, highlighting systems that are running over the scheduled hours. This snapshot view allows building management to look quickly at the entire campus to ensure that systems are not operating beyond their scheduled hours

Seymour Aquifer Water Quality Improvement Project Final Report

The Seymour Aquifer is a shallow aquifer underlying over 300,000 acres in 20 counties in northwest central Texas. High nitrate concentrations are widespread in the Seymour Aquifer. Median nitrate levels in Knox, Haskell, Baylor, Hall, Wichita, Wilbarger, and Fisher counties exceeded the federal safe drinking water standard (10 mg/L NO3-N). This high concentration is a concern because although 90% of the water pumped from the aquifer is used for irrigation, it is also used as a municipal water source for the communities of Vernon, Burkburnett, and Electra and rural families in the region. To address this threat, the Texas State Soil and Water Conservation Board (TSSWCB), with 319(h) grant funding provided by the Environmental Protection Agency (EPA), worked cooperatively with the Haskell, Wichita-Brazos, and California Creek Soil and Water Conservation Districts (SWCDs); U.S. Department of Agriculture - Natural Resources Conservation Service (NRCS); Texas AgriLife Extension Service (AgriLife Extension); Texas A&M AgriLife – Texas Water Resources Institute (TWRI); Rolling Plains Groundwater Conservation District; and Texas AgriLife Research (AgriLife Research) to encourage the installation of subsurface drip irrigation (SDI) systems and other best management practices (BMPs) to improve water quality (i.e. reduce nitrate) and increase water quantity in the Seymour Aquifer. The project provided technical and financial assistance to producers to implement SDI and other BMPs, education programs and demonstrations of methods for reducing nitrate infiltration and improving irrigation efficiency and an evaluation of the effectiveness of SDI implementation. Considerable interest has been generated in SDI and other more efficient irrigation methods through the efforts of project partners. Through technical and financial assistance provided by the project through the TSSWCB and Haskell, Wichita-Brazos and California Creek SWCDs, 17 producers installed SDI systems on over 1,000 acres. In addition, irrigation management was implemented through the Water Quality Management Plans (WQMPs) developed on over 1,800 acres and nutrient management was implemented on over 2,500 acres. NRCS also began funding irrigation improvements in Haskell, Knox, Baylor, Wilbarger, Hardeman and Foard counties through the Seymour Aquifer Special Emphasis Area. Since this Special Emphasis Area was established in 2004, over $16 million dollars have been provided. In addition to implementing BMPs, a very important component of this project was conducting educational programs and demonstrations. Through seven programs conducted between 2005 and 2008, AgriLife Research and AgriLife Extension provided educational programs and demonstrations on nutrient management and irrigation management to 671 participants. The establishment of the permanent SDI demonstration site at the Chillicothe Station will ensure that these programs are sustained for many years to come and offer producers in the Texas Rolling Plains additional crop production options to enhance economic returns and water quality, and improve their quality of life

Improved Evolutionary Hybrids for Flexible Ligand Docking in Autodock

In this paper we evaluate the design of the hybrid evolutionary algorithms (EAs) that are currently used to perform flexible ligand binding in the Autodock docking software. Hybrid EAs incorporate specialized operators that exploit domain-specific features to accelerate an EA's search. We consider hybrid EAs that use an integrated local search operator to reline individuals within each iteration of the search. We evaluate several factors that impact the efficacy of a hybrid EA, and we propose new hybrid EAs that provide more robust convergence to low-energy docking configurations than the methods currently available in Autodock

Appropriateness and timeliness of care-seeking for complications of pregnancy and childbirth in rural Ethiopia: a case study of the Maternal and Newborn Health in Ethiopia Partnership

Background: In 2014, USAID and University Research Co., LLC, initiated a new project under the broader Translating Research into Action portfolio of projects. This new project was entitled Systematic Documentation of Illness Recognition and Appropriate Care Seeking for Maternal and Newborn Complications. This project used a common protocol involving descriptive mixed-methods case studies of community projects in six low- and middle-income countries, including Ethiopia. In this paper, we present the Maternal and Newborn Health in Ethiopia Partnership (MaNHEP) case study. Methods: Methods included secondary analysis of data from MaNHEP\u2019s 2010 baseline and 2012 end line surveys, health program inventory and facility mapping to contextualize care-seeking, and illness narratives to identify factors influencing illness recognition and care-seeking. Analyses used descriptive statistics, bivariate tests, multivariate logistic regression, and thematic content analysis. Results: Maternal illness awareness increased between 2010 and 2012 for major obstetric complications. In 2012, 45% of women who experienced a major complication sought biomedical care. Factors associated with care-seeking were MaNHEP CMNH Family Meetings, health facility birth, birth with a skilled provider, or health extension worker. Between 2012 and 2014, the Ministry of Health introduced nationwide initiatives including performance review, ambulance service, increased posting of midwives, pregnant women\u2019s conferences, user-friendly services, and maternal death surveillance. By 2014, most facilities were able to provide emergency obstetric and newborn care. Yet in 2014, biomedical care-seeking for perceived maternal illness occurred more often compared with care-seeking for newborn illness\u2014a difference notable in cases in which the mother or newborn died. Most families sought care within 1 day of illness recognition. Facilitating factors were health extension worker advice and ability to refer upward, and health facility proximity; impeding factors were time of day, weather, road conditions, distance, poor cell phone connectivity (to call for an ambulance), lack of transportation or money for transport, perceived spiritual or physical vulnerability of the mother and newborn and associated culturally determined postnatal restrictions on the mother or newborn\u2019s movement outside of the home, and preference for traditional care. Some families sought care despite disrespectful, poor quality care. Conclusions: Improvements in illness recognition and care-seeking observed during MaNHEP have been reinforced since that time and appear to be successful. There is still need for a concerted effort focusing on reducing identified barriers, improve quality of care and provider counseling, and contextualize messaging behavior change communications and provider counseling

Macrophage metallothioneins participate in the antileishmanial activity of antimonials

Host cell functions that participate in the pharmacokinetics and pharmacodynamics (PK/PD) of drugs against intracellular pathogen infections are critical for drug efficacy. In this study, we investigated whether macrophage mechanisms of xenobiotic detoxification contribute to the elimination of intracellular Leishmania upon exposure to pentavalent antimonials (SbV). Primary macrophages from patients with cutaneous leishmaniasis (CL) (n=6) were exposed ex vivo to L. V. panamensis infection and SbV, and transcriptomes were generated. Seven metallothionein (MT) genes, potent scavengers of heavy metals and central elements of the mammalian cell machinery for xenobiotic detoxification, were within the top 20 up-regulated genes. To functionally validate the participation of MTs in drug-mediated killing of intracellular Leishmania, tandem knockdown (KD) of MT2-A and MT1-E, MT1-F, and MT1-X was performed using a pan-MT shRNA approach in THP-1 cells. Parasite survival was unaffected in tandem-KD cells, as a consequence of strong transcriptional upregulation of MTs by infection and SbV, overcoming the KD effect. Gene silencing of the metal transcription factor-1 (MTF-1) abrogated expression of MT1 and MT2-A genes, but not ZnT-1. Upon exposure to SbV, intracellular survival of Leishmania in MTF-1KD cells was significantly enhanced. Results from this study highlight the participation of macrophage MTs in Sb-dependent parasite killing

Endogenous ribosomal frameshift signals operate as mRNA destabilizing elements through at least two molecular pathways in yeast

Although first discovered in viruses, previous studies have identified operational −1 ribosomal frameshifting (−1 RF) signals in eukaryotic genomic sequences, and suggested a role in mRNA stability. Here, four yeast −1 RF signals are shown to promote significant mRNA destabilization through the nonsense mediated mRNA decay pathway (NMD), and genetic evidence is presented suggesting that they may also operate through the no-go decay pathway (NGD) as well. Yeast EST2 mRNA is highly unstable and contains up to five −1 RF signals. Ablation of the −1 RF signals or of NMD stabilizes this mRNA, and changes in −1 RF efficiency have opposing effects on the steady-state abundance of the EST2 mRNA. These results demonstrate that endogenous −1 RF signals function as mRNA destabilizing elements through at least two molecular pathways in yeast. Consistent with current evolutionary theory, phylogenetic analyses suggest that −1 RF signals are rapidly evolving cis-acting regulatory elements. Identification of high confidence −1 RF signals in ∼10% of genes in all eukaryotic genomes surveyed suggests that −1 RF is a broadly used post-transcriptional regulator of gene expression

SWEET11b transports both sugar and cytokinin in developing barley grains

Even though Sugars Will Eventually be Exported Transporters (SWEETs) have been found in every sequenced plant genome, a comprehensive understanding of their functionality is lacking. In this study, we focused on the SWEET family of barley (Hordeum vulgare). A radiotracer assay revealed that expressing HvSWEET11b in African clawed frog (Xenopus laevis) oocytes facilitated the bidirectional transfer of not only just sucrose and glucose, but also cytokinin. Barley plants harboring a loss-of-function mutation of HvSWEET11b could not set viable grains, while the distribution of sucrose and cytokinin was altered in developing grains of plants in which the gene was knocked down. Sucrose allocation within transgenic grains was disrupted, which is consistent with the changes to the cytokinin gradient across grains, as visualized by magnetic resonance imaging and Fourier transform infrared spectroscopy microimaging. Decreasing HvSWEET11b expression in developing grains reduced overall grain size, sink strength, the number of endopolyploid endosperm cells, and the contents of starch and protein. The control exerted by HvSWEET11b over sugars and cytokinins likely predetermines their synergy, resulting in adjustments to the grain's biochemistry and transcriptome