Performances thermiques d'une cuve agitée munie d'un agitateur de proximité de type hélicoïdal traitant des fluides alimentaires Newtoniens

National audienc

Performances thermiques d'une cuve agitée munie d'un agitateur de proximité de type hélicoïdal traitant des fluides alimentaires Newtoniens

National audienc

Confrontation expérimentale de trois méthodes de traçage pour la détermination du temps de circulation axiale moyen d'un agitateur helicoïdal

National audienc

Effet de la position du mobile par rapport au fond de la cuve sur la consommation de puissance de différents agitateurs plans verticaux

National audienc

Confrontation expérimentale de trois méthodes de traçage pour la détermination du temps de circulation axiale moyen d'un agitateur helicoïdal

National audienc

Numerical simulation of flow of newtonian fluids in an agitated vessel equipped with a non standard helical ribbon impeller

International audienc

Préparation de fruits sur sucre en cuves mécaniquement agitées et en continu

National audienc

Experimental and CFD Simulation of Heat Transfer to Highly Viscous Fluids in an Agitated Vessel Equipped With a non Standard Helical Ribbon Impeller

International audienc

Acceleration of keratinocyte differentiation by transient receptor potential vanilloid (TRPV6) channel activation

International audienceBackground: Numerous studies have demonstrated the beneficial effect of Avène Thermal Spring Water (TSW) in dermatological diseases but the molecular mechanisms remain unknown. The objective of the present study was to evaluate the effect of Avène TSW on the morphological and molecular features related to the more advanced status of differentiation of human keratinocytes.Material and methods: Normal human keratinocytes (NHK) were differentiated in medium powder reconstituted with Avène TSW and assessed by RT-PCR and immunohistochemistry. Calcium entry was measured by a Fura-2 AM probe. TRPV6 channel were detected by immunohistochemistry, RT-PCR and western blot.Results: Treatment of NHK with Avène TSW led to an enhanced constitutive calcium entry that resulted in the increased expression of involucrin and cytokeratins 1 and 10. This enhanced constitutive calcium entry in Avène TSW-treated keratinocytes was mediated by the TRPV6 calcium channel. Moreover, Avène TSW-mediated calcium entry was due to the increase in TRPV6 expression as well as the channel abundance at the cell membrane.Conclusions: An other mechanism of action of Avène TSW is described. Avène TSW treatment induced an enhanced constitutive calcium entry mediated by TRPV6 channel leading to the acceleration of human keratinocytes differentiation