33 research outputs found
Detection of Indicator Bacteria and Pathogens in Water by Polym Erase Chain Reaction (PCR) and Gene Probe Methods
No full textDetection of viable bacteria in environmental water samples using DNase I and PCR method
No full textUltra sensitive detection of Listeria monocytogenes in milk by the polymerase chain reaction (PCR)
No full textPCR products generated from unpurified Salmonella DNA are degraded by thermostable nuclease activity
No full textBacterial gene expression at cold temperatures
No full textUnder suboptimal environmental conditions such as low temperatures, many bacteria have an extended lag phase, altered cell structures, and composition such as a less fluid (more rigid) and leaky cytoplasmic membrane. As a result, cells may die, enter into a starvation mode of metabolism or a physiologically viable but non-culturable (VBNC) state. In the latter state, the amount of gene expression per cell is virtually undetectable. In this article, gene expression under (suboptimal) low temperature conditions in non-psychrophilic environmental bacteria is examined. The pros and cons of some of the molecular methodologies for gene expression analysis are also discusse
Detection of low numbers of Salmonella in environmental water, sewage and food samples by a nested polymerase chain reaction assay
No full textApplication of the polymerase chain reaction to the identification of Escherichia coli and coliforms in water
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