Cleavage resistant photoluminescent proteins and applications thereof

Photoluminescent proteins resistant to protein cleavage reagents are described herein. In being resistant to cleavage reagents, such photoluminesncent proteins can reduce the number of digestion or cleavage products, thereby simplifying quantification and purification of recombinant protein compositions. In some embodiments, a photoluminescent protein described herein comprises an amino acid sequence having an absence of internal methionine residues

Microvolume immunoabsorbant assays with amplified electrochemical detection

Describes a structure and method for chemical sensing utilizing microassays. Microcavities or micropores are combined with assay techniques to provide a very fast and very sensitive means of detecting chemical compounds. Assay techniques are modified to include a metal ion binding carrier species especially suitable for use in conjunction with the electrochemical detection. This allows assays to be combined with electrochemical analysis, thus allowing the high speed ease and hypersensitivity available in the invention

Electrochemical method for detecting water born pathogens

A novel surface immobilization electrochemical assay that allows for rapid, accurate and highly sensitive detection of microorganisms and biological molecules is described. Known surface immobilization methods are utilized to bind an analyte to a surface. A binding material with a covalently attached electroactive complex generates electrical current in the presence of analyte. An electrode is used to detect the current that is directly related to the concentration of analyte. The invention is especially suitable for detection of Cryptosporidium parvum

Self-contained microelectrochemical bioassay platforms and methods

The present invention relates to a microvolume electrochemical detection assay. More specifically, the present invention relates to microscale structures having an analyte immobilizing surface and at least one electrode separate from the immobilizing surface. The microscale devices are used in conjunction with an immobilization assay that utilizes an electroactive complex to generate a current that may be detected by the electrode. The small volumes and sensitivity of the assays used in conjunction with microstructures provides an extremely rapid detection method having superior sensitivity to existing methods

Self-contained microelectrochemical bioassay platforms and methods

Describes methods and devices for improved chemical and biomass detection assays that combine well defined microstructures having independently addressable electrodes with various surface immobilization electrochemical assays. Combining known chemical detection immobilization assays, electrochemically active moieties with microstructures having independently addressable electrodes provides for vastly improved methods of detecting microorganisms, chemical compounds, and measuring membrane transport

E. coli separatome-based protein expression and purification platform

Provided is a separatome-based peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome of E. coli. The separatome-based protein expression and purification platform quantitatively describes and identifies priority deletions, modifications, or inhibitions of certain gene products to increase chromatographic separation efficiency, defined as an increase in column capacity, column selectivity, or both, with emphasis on the former. Moreover, the separatome-based protein expression and purification platform provides a computerized knowledge tool that, given separatome data, and a target recombinant peptide, polypeptide, or protein, intuitively suggests strategies facilitating efficient product purification. The separatome-based protein expression and purification platform is an efficient bioseparation system that intertwines host cell expression systems and chromatography

User interface for software applications

A user interface for software applications enables the full use of graphical user interface (GUI) software, particularly engineering design software, by visually impaired individuals is described. The interface combines tactile representations of graphical elements, non-visual cues, and a hardware element to allow the effective placement and interconnection of graphic elements using design software

Trans-, trans-conjugated linoleic acid compositions and use thereof

The invention relates generally to trans-,trans-conjugated linoleic acid compositions and their use , in particular to trans-,trans-conjugated linoleic acid produced from photoisomerization of soy oil in the presence of an antioxidant, and are used in pharmaceutical, nutraceutical and human and animal food compositions for improved health. The invention also relates generally to a method for treatment and prevention of health-related disorders of obesity by administration of pharmaceutical, nutraceutical and/or food compositions containing and/or prepared from trans-,trans-conjugated linoleic acid-rich soy oil

Separatome-based protein expression and purification platform

Provided is a separatome-based recombinant peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome, such as that of E. coli, yeast, Bacillus subtilis or other prokaryotes, insect cells, mammalian cells, etc. This platform quantitatively describes and identifies priority deletions, modifications, or inhibitions of certain gene products to increase chromatographic separation efficiency, defined as an increase in column capacity, column selectivity, or both, with emphasis on the former. Moreover, the platform provides a computerized knowledge tool that, given separatome data and a target recombinant peptide, polypeptide, or protein, intuitively suggests strategies leading to efficient product purification. The separatome-based protein expression and purification platform is an efficient bioseparation system that intertwines host cell expression systems and chromatography