19 research outputs found

    Enterobius vermicularis Salpingitis Seen in the Setting of Ectopic Pregnancy in a Malaysian Patient

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    We report a rare and unusual case of invasive Enterobius vermicularisinfection in a fallopian tube. The patient was a 23-year-old Malaysian woman who presented with suprapubic pain and vaginal bleeding. A clinical diagnosis of ruptured right ovarian ectopic pregnancy was made. She underwent a laparotomy with a right salpingo-oophorectomy. Histopathological examination of the right fallopian tube showed eggs and adult remnants of E. vermicularis, and the results were confirmed using PCR and DNA sequencing

    TOURISM PLANNING

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    Bachelor'sBACHELOR OF SCIENCE (ESTATE MANAGEMENT

    POST-HARVEST PHYSIOLOGY OF TROPICAL FRUITS

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    Master'sMASTER OF SCIENC

    SINGAPORE'S INLAND TRANSPORTATION

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    Bachelor'sBACHELOR OF SOCIAL SCIENCES (HONOURS

    Photosynthesis and antioxidant enzymes of phyllodes of Acacia mangium. Plant Sci

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    Abstract Physiological processes are influenced by environmental factors and plant characteristics. The distribution of photosynthetic capacity of phyllodes of Acacia mangium Willd. seedlings was studied in relation to the in vivo photosystem II (PSII) function, photosynthetic gas exchange, chlorophyll fluorescence and activities of antioxidant enzymes (superoxide dismutase (SOD) and ascorbate peroxidase (APX)) of phyllodes at different positions on seedlings. There was a vertical gradient in photosynthetic capacity of phyllodes along the shoot. Phyllode 1 (at the apex) showed negative carbon uptake at PPFD lower than 400 mmol m − 2 s − 1 . High photosynthetic capacities, chlorophyll concentrations, DF/F% m , and q P were observed in phyllodes 4, 6 and 8. The high photosynthetic capacities of mature phyllodes could be attributed to the enhanced availability of CO 2 and the high efficiency of PSII in energy absorption and utilization. Total SOD and APX activities (on a dry weight basis) were highest at phyllode 1 and decreased as the phyllodes matured. The high photosynthetic capacity and low respiration loss in mature phyllodes could be important factors, responsible for the rapid establishment and fast growth of A. mangium in reforestation programs

    Photosynthesis in Platycerium coronarium (Koenig ex Mueller) Desv.

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    Photosynthetica31159-69PHSY

    Polymorphisms in methylenetetrahydrofolate reductase gene and risk of non-Hodgkin lymphoma in a multi-ethnic population

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    An imbalance in folate metabolism can adversely affect DNA synthesis and methylation systems which can lead to susceptibility to non-Hodgkin lymphoma (NHL). Whether single nucleotide polymorphisms (SNPs) and their haplotypes in the methylenetetrahydrofolate reductase (MTHFR) are associated with NHL, remain inconclusive. We investigated the association between MTHFR C677T and A1298C SNPs and NHL risk in a population which is made up of Malay, Chinese and Indian ethnic subgroups. A total of 372 NHL patients and 722 controls were genotyped using the Sequenom MassARRAY platform. Our results of the pooled subjects failed to demonstrate significant association between the MTHFR C677T and A1298C SNPs with NHL and its subtypes. The results were in agreement with the previous meta-analyses. In the Indian ethnic subgroup however, single locus analysis of MTHFR A1298C appears to confer risk to NHL (Odds ratio (OR) 1.91, 95% confidence interval (95% CI) 1.22-3.00, P ¼ 0.006). The risk is almost doubled in homozygous carrier of MTHFR 1298CC (OR 4.03, 95% CI 1.56-10.43, P ¼ 0.004). Haplotype analysis revealed higher frequency of CC in the Indian NHL patients compared with controls (OR 1.86, 95% CI 1.18-2.93, P ¼ 0.007). There is lack of evidence to suggest an association between MTHFR C677T and A1298C with the risk of NHL in the Malays and Chinese. In the Indians however, the MTHFR A1298C confers risk to NHL. This study suggests ethnicity modifies the relationship between polymorphisms in the folate-metabolizing gene and NHL

    Molecular characterisation of the tick Rhipicephalus microplus in Malaysia: new insights into the cryptic diversity and distinct genetic assemblages throughout the world

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    Abstract Background The morphotaxonomy of Rhipicephalus microplus complex has been challenged in the last few years and prompted many biologists to adopt a DNA-based method for distinguishing the members of this group. In the present study, we used a mitochondrial DNA analysis to characterise the genetic assemblages, population structure and dispersal pattern of R. microplus from Southeast Asia, the region where the species originated. Methods A phylogeographic analysis inferred from the 16S rRNA and cytochrome oxidase subunit I (COI) genes was performed with five populations of R. microplus collected from cattle in Malaysia. Malaysian R. microplus sequences were compared with existing COI and 16S rRNA haplotypes reported globally in NCBI GenBank. Results A total of seven and 12 unique haplotypes were recovered by the 16S rRNA and COI genes, respectively. The concatenated sequences of both 16S rRNA and COI revealed 18 haplotypes. Haplotype network and phylogenetic analyses based on COI+16S rRNA sequences revealed four genetically divergent groups among Malaysian R. microplus. The significantly low genetic differentiation and high gene flow among Malaysian R. microplus populations supports the occurrence of genetic admixture. In a broader context, the 16S rRNA phylogenetic tree assigned all isolates of Malaysian R. microplus into the previously described African/the Americas assemblage. However, the COI phylogenetic tree provides higher resolution of R. microplus with the identification of three main assemblages: clade A sensu Burger et al. (2014) comprises ticks from Southeast Asia, the Americas and China; clade B sensu Burger et al. (2014) is restricted to ticks that originated from China; and clade C sensu Low et al. (2015) is a new genetic assemblage discovered in this study comprising ticks from India and Malaysia. Conclusions We conclude that the R. microplus complex consisting of at least five taxa: R. australis, R. annulatus, R. microplus clade A sensu Burger et al. (2014), R. microplus clade B sensu Burger et al. (2014) and the new taxon, R. microplus clade C sensu Low et al. (2015). The use of COI as the standard genetic marker in discerning the genetic assemblages of R. microplus from a broad range of biogeographical regions is proposed
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