Mapping geographical areas at risk for tick-borne encephalitis (TBE) by analysing bulk tank milk from Swedish dairy cattle herds for the presence of TBE virus-specific antibodies

Background The vector-borne human viral zoonosis tick-borne encephalitis (TBE) is of growing concern in Sweden. The area where TBE is considered endemic has expanded, with an increasing geographical distribution of Ixodes ricinus as the tick vector and a rising number of reported TBE cases in humans. Efforts to map TBE risk areas have been carried out by sentinel monitoring, mainly based on individual sampling and analysis of wild and domestic animals, as well as ticks, for tick-borne encephalitis virus (TBEV). However, the interpretation of the geographical distribution has been hampered by the patchy and focal nature of TBEV occurrence. This study presents TBEV surveillance data based on antibody analysis of bulk tank milk collected from dairy herds located throughout Sweden before (May) and after (November) the vector season. A commercial TBEV antibody ELISA was modified and evaluated for use in this study. Results The initial comparative TBEV antibody analysis revealed a good correlation between milk and serum antibody levels from individually sampled cows. Also, the TBEV-antibody levels for the mean-herd serum showed good comparability with TBEV antibody levels from bulk tank milk, thus indicating good predictability of seroprevalence when analysing bulk tank milk from a herd. Analyses of bulk tank milk samples collected from 616 herds in May and 560 herds in November showed a geographical distribution of TBEV seropositive herds that was largely consistent with reported human TBE cases. A few TBEV-reactive herds were also found outside known locations of human TBE cases. Conclusion Serological examination of bulk tank milk from dairy cattle herds may be a useful sentinel surveillance method to identify geographical presence of TBEV. In contrast to individual sampling this method allows a large number of animals to be monitored. TBEV seropositive herds were mainly found in coastal areas of southern Sweden similar to human TBE cases. However, some antibody-reactive herds were found outside known TBE areas at the time of the study

West Nile virus: diagnosis, surveillance and epidemiology in Europe

West Nile virus is an arbovirus affecting horses and humans, highly susceptible incidental and deadend hosts. Its presence in Europe is not new, as West Nile virus infections were first reported in the 1960’s: at the time, human and equine cases had already been described in France in the Camargue region. After 30 years without any reported outbreak, West Nile virus re-emerged in Europe at the end of the 90’s with limited (Czech Republic 1997, Italy 1998, France 2000) or large-scale sporadic outbreaks (Romania 1996, Russia 1999). More recently, a resurgence of WNV has been observed since 2008, probably related to changes in the multiple viral strains present in Europe, and in ecological and climatic conditions favourable to the multiplication of vector mosquitoes. This resurgence peaked in 2010, with unprecedented numbers of equine outbreaks and human cases in numerous countries in Europe, particularly in the Mediterranean basin: 8 countries (Bulgaria, Spain, Greece, Hungary, Italy, Portugal, Romania and Russia) were affected, with large-scale foci in Greece and Russia. Even though an equine vaccine is available in Europe since 2009, the control of West Nile Virus infection still relies heavily on reinforced surveillance of neurological conditions in humans and horses.Le virus West Nile est un arbovirus pouvant infecter le cheval et l'Homme, hôtes accidentels sensibles et culs-de-sac épidémiologiques. Sa présence en Europe est ancienne, puisque les premiers rapports d'infection par le virus West Nile datent des années 1960: à cette époque, des cas équins et humains avaient déjà été décrits en France dans la région de la Camargue. Après un long silence de plus de 30 ans, le virus West Nile a réémergé en Europe à la fin des années 1990, causant des foyers sporadiques d'ampleur limitée (République Tchèque 1997, Italie 1998, France 2000) à importante (Roumanie 1996, Russie 1999). Plus récemment, un regain d'activité du virus West Nile a été observé à partir de 2008, probablement dû à une évolution des multiples souches virales présentes en Europe, ainsi qu'à des conditions écologiques et climatiques favorables à la multiplication des moustiques vecteurs. Il a culminé, en 2010, avec une flambée d'épizooties équines et de cas humains sans précédent dans plusieurs pays européens, notamment du pourtour méditerranéen: huit pays (Bulgarie, Espagne, Grèce, Hongrie, Italie, Portugal, Roumanie et Russie) ont été touchés, avec des foyers d'ampleur considérable en Grèce et en Russie. Dans ce contexte européen en pleine évolution, et même si un vaccin est disponible pour l'espèce équine en Europe depuis 2009, la lutte contre l'infection par le virus West Nile continue de reposer sur une surveillance renforcée des affections neurologiques chez l'homme et le cheval

Identification of hotspots in the European Union for the introduction of four zoonotic arboviroses by live animal trade

Live animal trade is considered a major mode of introduction of viruses from enzootic foci into disease-free areas. Due to societal and behavioural changes, some wild animal species may nowadays be considered as pet species. The species diversity of animals involved in international trade is thus increasing. This could benefit pathogens that have a broad host range such as arboviruses. The objective of this study was to analyze the risk posed by live animal imports for the introduction, in the European Union (EU), of four arboviruses that affect human and horses: Eastern and Western equine encephalomyelitis, Venezuelan equine encephalitis and Japanese encephalitis. Importation data for a five-years period (2005-2009, extracted from the EU TRACES database), environmental data (used as a proxy for the presence of vectors) and horses and human population density data (impacting the occurrence of clinical cases) were combined to derive spatially explicit risk indicators for virus introduction and for the potential consequences of such introductions. Results showed the existence of hotspots where the introduction risk was the highest in Belgium, in the Netherlands and in the north of Italy. This risk was higher for Eastern equine encephalomyelitis (EEE) than for the three other diseases. It was mainly attributed to exotic pet species such as rodents, reptiles or cage birds, imported in small-sized containments from a wide variety of geographic origins. The increasing species and origin diversity of these animals may have in the future a strong impact on the risk of introduction of arboviruses in the EU. (Résumé d'auteur

Pathogens in ticks collected from dogs in Berlin/Brandenburg, Germany

BackgroundTick-borne diseases are a major health risk for humans and dogs. In addition to collection and analysis of questing ticks, analysis of host- associated ticks for the presence of pathogens is a valuable method to gain insight into transmission patterns of tick-borne diseases.MethodsTicks were collected from dogs living in the Berlin/Brandenburg area. The three tick species Ixodes ricinus, Ixodes hexagonus and Dermacentor reticulatus were examined for the presence of Babesia spp., Borrelia spp., Rickettsia spp. and Anaplasmataceae. Conventional PCR followed by sequencing was used for pathogen detection and characterization.Results Babesia spp. were found in 2.5% and 3% of I. ricinus and I. hexagonus, respectively. Sequencing revealed the presence of Babesia microti, Babesia capreoli and Babesia venatorum. D. reticulatus were free of Babesia canis. Rickettsia spp. were detected in 61% of I. ricinus, 44% of I. hexagonus and 39% of D. reticulatus. Specifically detected were Rickettsia raoulti in D. reticulatus and I. hexagonus, Rickettsia helvetica in I. ricinus and I. hexagonus and Rickettsia monacensis in I. hexagonus. Anaplasma phagocytophilum and Candidatus Neoehrlichia mikurensis have been reported previously in I. ricinus (6.5% and 4.3%, respectively) and I. hexagonus (3.9% and 5.9%). Borrelia spp. were found in 11.6% of I. ricinus and 11.2% of I. hexagonus. Subsequent genospecies analysis revealed Borrelia afzelii, Borrelia garinii, Borrelia burgdorferi sensu stricto and Borrelia miyamotoi. Simultanous presence of more than one pathogen was found in 20% of I. ricinus and in 59% of I. hexagonus whereas the total frequency of any pathogen was 65% in I. ricinus, 59% in I. hexagonus and 64% in D. reticulatus. Ticks in which A. phagocytophilum was detected had a significantly increased risk of also containing Rickettsia. Ticks harbouring a pathogen had significantly higher scutal indices than ticks without presence of any pathogen.ConclusionsFrequencies of potential human or canine pathogens in ticks were considerable and DNA of all four groups of pathogens was detected. Differences in scutal indices might suggest that pathogens are frequently taken up by ticks when feeding on dogs in Berlin/Brandenburg

Agent-based simulation of pedestrians' earthquake evacuation; application to Beirut, Lebanon

Most seismic risk assessment methods focus on estimating the damages to the built environment and the consequent socioeconomic losses without fully taking into account the social aspect of risk. Yet, human behaviour is a key element in predicting the human impact of an earthquake, therefore, it is important to include it in quantitative risk assessment studies. In this study, an interdisciplinary approach simulating pedestrians' evacuation during earthquakes at the city scale is developed using an agent-based model. The model integrates the seismic hazard, the physical vulnerability as well as individuals' behaviours and mobility. The simulator is applied to the case of Beirut, Lebanon. Lebanon is at the heart of the Levant fault system that has generated several Mw>7 earthquakes, the latest being in 1759. It is one of the countries with the highest seismic risk in the Mediterranean region. This is due to the high seismic vulnerability of the buildings due to the absence of mandatory seismic regulation until 2012, the high level of urbanization, and the lack of adequate spatial planning and risk prevention policies. Beirut as the main residential, economic and institutional hub of Lebanon is densely populated. To accommodate the growing need for urban development, constructions have almost taken over all of the green areas of the city; squares and gardens are disappearing to give place to skyscrapers. However, open spaces are safe places to shelter, away from debris, and therefore play an essential role in earthquake evacuation. Despite the massive urbanization, there are a few open spaces but locked gates and other types of anthropogenic barriers often limit their access. To simulate this complex context, pedestrians' evacuation simulations are run in a highly realistic spatial environment implemented in GAMA [1]. Previous data concerning soil and buildings in Beirut [2, 3] are complemented by new geographic data extracted from high-resolution Pleiades satellite images. The seismic loading is defined as a peak ground acceleration of 0.3g, as stated in Lebanese seismic regulations. Building damages are estimated using an artificial neural network trained to predict the mean damage [4] based on the seismic loading as well as the soil and building vibrational properties [5]. Moreover, the quantity and the footprint of the generated debris around each building are also estimated and included in the model. We simulate how topography, buildings, debris, and access to open spaces, affect individuals' mobility. Two city configurations are implemented: 1. Open spaces are accessible without any barriers; 2. Access to some open spaces is blocked. The first simulation results show that while 52% of the population is able to arrive to an open space within 5 minutes after an earthquake, this number is reduced to 39% when one of the open spaces is locked. These results show that the presence of accessible open spaces in a city and their proximity to the residential buildings is a crucial factor for ensuring people's safety when an earthquake occurs

Entry-competent-replication-abortive African horse sickness virus strains elicit robust immunity in ponies against all serotypes

African horse sickness virus (AHSV) is an Orbivirus within the Reoviridae family, spread by Culicoides species of midges, which infects equids with high mortality, particularly in horses and has a considerable impact on the equine industry. In order to control the disease, we previously described Entry Competent Replication Abortive (ECRA) virus strains for each of the nine distinct AHSV serotypes and demonstrated their potential as vaccines, first in type I interferon receptor (IFNAR-/-) knockout mice, and then in ponies. In this report we have investigated whether or not a combination ECRA vaccine comprising nine vaccine strains as two different cocktails is as efficient in ponies and the duration of the immunity triggered by ECRA vaccines. In one study, a group of ponies were vaccinated with a cocktail of 4 vaccine strains, followed by a vaccination of the remaining 5 vaccine strains, mimicking the current live attenuated vaccine regimen. In the second study, ponies were vaccinated with a single ECRA-AHSV strain and monitored for 6 months. The first group of ponies developed neutralising antibody responses against all 9 serotypes, indicating that no cross-serotype interference occurred, while the second group developed robust neutralising antibody responses against the single serotype that were sustained at the same level throughout a 6-month study. The results support our previous data and further validate ECRA vaccines as a safe and efficacious replacement of current live vaccines

Widespread Circulation of Flaviviruses in Horses and Birds in Northeastern Spain (Catalonia) between 2010 and 2019

The surveillance for West Nile virus (WNV) in Catalonia (northeastern Spain) has consistently detected flaviviruses not identified as WNV. With the aim of characterizing the flaviviruses circulating in Catalonia, serum samples from birds and horses collected between 2010 and 2019 and positive by panflavivirus competition ELISA (cELISA) were analyzed by microneutralization test (MNT) against different flaviviruses. A third of the samples tested were inconclusive by MNT, highlighting the limitations of current diagnostic techniques. Our results evidenced the widespread circulation of flaviviruses, in particular WNV, but also Usutu virus (USUV), and suggest that chicken and horses could serve as sentinels for both viruses. In several regions, WNV and USUV overlapped, but no significant geographical aggregation was observed. Bagaza virus (BAGV) was not detected in birds, while positivity to tick-borne encephalitis virus (TBEV) was sporadically detected in horses although no endemic foci were observed. So far, no human infections by WNV, USUV, or TBEV have been reported in Catalonia. However, these zoonotic flaviviruses need to be kept under surveillance, ideally within a One Health framework

Le virus Usutu : la menace fantôme

International audienc

Undetection of vector-borne viruses in equids of Galapagos Islands

Domestic species, including equids, were introduced in the Galapagos Islands in the XIX century. Equine vector-borne diseases are circulating in South America but their occurrence in the Galapagos Island was unknown. The objective of this study was to detect the occurrence of West Nile virus (WNV), Usutu virus (USUV) and equine infectious anemia virus (EIAV) in the four Galapagos Islands raising equids if they were present at a prevalence >1%. Serum samples were collected from 411 equids belonging to 124 owners from April to July 2019. All the results were negative to the ELISA tests used suggesting that WNV, USUV and EIAV are not circulating in the equine population of the Galapagos Islands