What Children Say: Results of Comparative Research on the Physical and Emotional Punishment of Children in South East Asia and the Pacific

This report was commisioned by Unknow

How are the human rights of children related to research methodology?

This chapter explores the relationship between discrimination and rights, and how this relationship is used in developing a conceptual framework that can be of practical use in the promotion of the rights of children. It explores, in a mainly UK context, what forms discrimination against children can take, the mechanisms via which it operates, and what its effects are on the lives and welfare of children. In another corner of the UK a second civil rights movement was gaining pace at much the same time, one that grew out of the systematic discrimination experienced by Roman Catholics in Northern Ireland, and which led to a 30-year civil war. The examples provide a clear link between human rights and discrimination, that is, upholding the human rights of vulnerable groups, is they ethnic or religious minorities, women, or people with disability, requires the identification and eradication of discrimination

In vivo and in vitro evidence for hydrogen peroxide (H2O2) accumulation in the epidermis of patients with vitiligo and its successful removal by a UVB-activated pseudocatalase

To date there is compelling in vitro and in vivo evidence for epidermal H2O2 accumulation in vitiligo. This paper reviews the literature and presents new data on oxidative stress in the epidermal compartment of this disorder. Elevated H2O2 levels can be demonstrated in vivo in patients compared with healthy controls by utilizing Fourier-Transform Raman spectroscopy. H2O2 accumulation is associated with low epidermal catalase levels. So far, four potential sources for epidermal H2O2 generation in vitiligo have been identified: (i) perturbed (6R)-L-erythro 5,6,7,8 tetrahydrobiopterin (6BH4) de novo synthesis/recycling/regulation; (ii) impaired catecholamine synthesis with increased monoamine oxidase A activities; (iii) low glutathione peroxidase activities; and (iv) "oxygen burst" via NADPH oxidase from a cellular infiltrate. H2O2 overproduction can cause inactivation of catalase as well as vacuolation in epidermal melanocytes and keratinocytes. Vacuolation was also observed in vitro in melanocytes established from lesional and nonlesional epidermis of patients (n = 10) but was reversible upon addition of catalase. H2O2 can directly oxidize 6BH4 to 6-biopterin, which is cytotoxic to melanocytes in vitro. Therefore, we substituted the impaired catalase with a "pseudocatalase". Pseudocatalase is a bis-manganese III-EDTA-(HCO3-)2 complex activated by UVB or natural sun. This complex has been used in a pilot study on 33 patients, showing remarkable repigmentation even in long lasting disease. Currently this approach is under worldwide clinical investigation in an open trial. In conclusion, there are several lines of evidence that the entire epidermis of patients with vitiligo is involved in the disease process and that correction of the epidermal redox status is mandatory for repigmentation