Dual expression recombinase based (DERB) single vector system for high throughput screening and verification of protein interactions in living cells

Identification of novel protein interactions and their mediators is fundamental in understanding cellular processes and is necessary for protein-targeted therapy. Evidently high throughput formatting of these applications in living cells would be beneficial, however no adequate system exists. We present a novel platform technology for the high throughput screening and verification of protein interactions in living cells. The platform's series of Dual Expression Recombinase Based (DERB) destiny vectors individually encode two sets of recombinase recognizable sequences for inserting the protein open reading frame (ORF) of interest, two sets of promoters and reporter tags in frame with the ORFs for detecting interactions. Introduction into living cells (prokaryotic and eukaryotic) enables the detection of protein interactions by fluorescence resonance energy transfer (FRET) or bimolecular fluorescence complementation (BiFC). The DERB platform shows advantages over current commercialized systems by DERB vectors validated through proof-of-principle experiments and the identification of an unknown interaction

Is there a "heat or eat" trade-off in the UK?

In this research, funded by the Nuffield Foundation, we merge detailed household level expenditure data from older households with historical local weather information. We then test for a heat or eat trade off: do households cut back on food spending to finance the additional cost of keeping warm during cold shocks? We find evidence that the poorest of older households are unable to smooth spending over the worst temperature shocks. Statistically significant reductions in food spending are observed in response to temperatures two or more standard deviations colder than expected (which occur about one winter month in forty) and reductions in food expenditure are considerably larger in poorer households.

Is There a Heat or Eat Trade-off in the UK?

We merge detailed household level expenditure data from older households with historical local weather information. We then test for a heat or eat trade off: do households cut back on food spending to finance the additional cost of keeping warm during cold shocks? For households who cannot smooth consumption over time, cold weather shocks are equivalent to income shocks. We find evidence that the poorest of older households are unable to smooth spending over the worst temperature shocks. Statistically significant reductions in food spending are observed in response to winter temperatures two or more standard deviations colder than expected (which occur about one winter month in forty) and reductions in food expenditure are considerably larger in poorer households.

Cash by any other name? Evidence on labelling from the UK Winter Fuel Payment

Standard economic theory implies that the labelling of cash transfers or cash-equivalents (e.g. child benefits, food stamps) should have no effect on spending patterns. The empirical literature to date does not contradict this proposition. We study the UK Winter Fuel Payment (WFP), a cash transfer to older households. Exploiting sharp eligibility criteria in a regression discontinuity design, we find robust evidence of a behavioural effect of the labelling. On average households spend 41% of the WFP on fuel. If the payment was treated as cash, we would expect households to spend approximately 3% of the payment on fuel.

Microbrewing and entrepreneurship : the origins, development and integration of real ale breweries in Britain

This paper reports on an exploratory two-stage study of microbreweries in the UK. The first stage comprises an analysis of data from the Small Independent Breweries Association to offer an aggregate picture of the sector. The second stage reports on a qualitative study of the experiences of 14 microbreweries. The findings from the fieldwork show that the UK microbrewing sector is growing, that competitiveness within the brewing establishment is based on artisan manufacture, provenance and diversity rather than price, and that the sector is contestable but operates as a competitive fringe within the greater industry. The study illustrates that microbreweries can contribute to local economies and that, because of the innovation, diversity and growth in the sector, entrepreneurship is in evidence. While saturation seems a threat, the evidence presented here suggests that UK microbrewing is a healthy sector, with the prospect of ongoing growth and contribution

Cryptic introgression into the kidney saxifrage (Saxifraga hirsuta) from its more abundant sympatric congener Saxifraga spathularis, and the potential risk of genetic assimilation

Background and Aims Although hybridization can play a positive role in plant evolution, it has been shown that excessive unidirectional hybridization can result in replacement of a species’ gene pool, and even the extinction of rare species via genetic assimilation. This study examines levels of introgression between the common Saxifraga spathularis and its rarer congener S. hirsuta, which have been observed to hybridize in the wild where they occur sympatrically. Methods Seven species-specific single nucleotide polymorphisms (SNPs) were analysed in 1025 plants representing both species and their hybrid, S. × polita, from 29 sites across their ranges in Ireland. In addition, species distribution modelling was carried out to determine whether the relative abundance of the two parental species is likely to change under future climate scenarios. Key Results Saxifraga spathularis individuals tended to be genetically pure, exhibiting little or no introgression from S. hirsuta, but significant levels of introgression of S. spathularis alleles into S. hirsuta were observed, indicating that populations exhibiting S. hirsuta morphology are more like a hybrid swarm, consisting of backcrosses and F(2)s. Populations of the hybrid, S. × polita, were generally comprised of F(1)s or F(2)s, with some evidence of backcrossing. Species distribution modelling under projected future climate scenarios indicated an increase in suitable habitats for both parental species. Conclusions Levels of introgression observed in this study in both S. spathularis and S. hirsuta would appear to be correlated with the relative abundance of the species. Significant introgression of S. spathularis alleles was detected in the majority of the S. hirsuta populations analysed and, consequently, ongoing introgression would appear to represent a threat to the genetic integrity of S. hirsuta, particularly in areas where the species exists sympatrically with its congener and where it is greatly outnumbered

Do schools and alcohol mix? Australian parents\u27 perspectives

© 2020, Emerald Publishing Limited. Purpose: Alcohol use by adults at school events and alcohol promotion through school fundraising activities is common, but little is known about secondary school parents\u27 attitudes towards these practices. Parental attitudes may influence principals\u27 decision-making on this topic, particularly in jurisdictions where education department guidance is limited. This study explored parents\u27 attitudes towards the consumption or promotion of alcohol in schools or at school events. Design/methodology/approach: Parents (n = 298) from five non-government secondary schools in Western Australia completed an online survey and provided responses relating to the promotion and availability of alcohol through their child\u27s school. Findings: This sample of parents were evenly divided in support of alcohol consumption or support of schools as alcohol-free zones. Parents reporting higher alcohol consumption were more supportive of alcohol promotion and use through schools, and those with higher education supported use of alcohol for school fundraising. Almost 20% of parents were neutral on several measures indicating they could be swayed by social pressure. Engaging parents is an ongoing challenge for school principals and alcohol may play a part in engagement activities. The results from this small, exploratory study suggest even engaged parents may have very differing views on alcohol use in schools. Practical implications: Education departments are encouraged to explore these issues carefully and introduce changes incrementally to assist decision-making and minimise potential parent disengagement. Originality/value: This paper addresses a knowledge gap about parents\u27 attitudes towards alcohol in secondary schools. These findings can support those involved in the development of school alcohol policies

Cash by any other name? Evidence on labeling from the UK Winter Fuel Payment

Government transfers to individuals are often given labels indicating that they are designed to support the consumption of particular goods. Standard economic theory implies that the labeling of cash transfers or cash-equivalents should have no effect on spending patterns. We study the UK Winter Fuel Payment, a cash transfer to older households. Our empirical strategy nests a regression discontinuity design within an Engel curve framework. We find robust evidence of a behavioral effect of labeling. On average households spend 47% of the WFP on fuel. If the payment were treated as cash, we would expect households to spend 3% of the payment on fuel. © 2014 Elsevier B.V

Nonprofit Sector Response to COVID-19

In an initial effort to understand the impact of the COVID-19 pandemic on local nonprofits’ ability to meet their community’s needs, The University of San Diego’s Nonprofit Institute issued a survey to nonprofit leaders on March 18, 2020. The aim of this report is to provide real-time data to government officials, foundations, and other decision-makers about the current economic conditions facing nonprofits and the need for immediate and long-term support in order to ensure the ongoing provision of critical services in the San Diego region.https://digital.sandiego.edu/npi-npissues/1003/thumbnail.jp

Cash by any other name? evidence on labelling from the UK Winter Fuel Payment

This presentation was delivered at the Geary Institute, University College Dublin