Isolasi Dan Identifikasi Bakteri Aerob Yang Berpotensi Menyebabkan Infeksi Nosokomial Di Irina E Rsup Prof. Dr. R. D. Kandou Manado

: Nosocomial infections or now called Healthcare Associated Infection (HAIs) is an infection that occurs in patients during treatment in a hospital or other health care facilities that do not show symptoms of infection while in the hospital. Nosocomial infection is an important issue in health care that continues to increase especially in children who are under treatment in hospital. This study aims to determine the source and identify aerobic bacteria that could potentially cause nosocomial infections in walls, floors, medical equipment, and air in Irina E Prof. Dr. R. D. Kandou Manado General Hospital. Design methods for this research using cross sectional study with a descriptive prospective approach. Samples were bacteria in walls, floors, medical equipment, and air of Irian E Prof. Dr. R. D. Kandou Manado General Hospital. The results showed that from the 24 samples taken, there were 6 bacteria found which is Enterobacter cloacae (41,7%), Bacillus subtilis (20,8%), Serratia liquefaciens (16,7%), Enterobacter agglomerans (12,5%), Lactobacillus (4,17%), dan Staphylococcus sp. (4,17%)

Maximizing nearest neighbour entanglement in finitely correlated qubit--chains

We consider translationally invariant states of an infinite one dimensional chain of qubits or spin-1/2 particles. We maximize the entanglement shared by nearest neighbours via a variational approach based on finitely correlated states. We find an upper bound of nearest neighbour concurrence equal to C=0.434095 which is 0.09% away from the bound C_W=0.434467 obtained by a completely different procedure. The obtained state maximizing nearest neighbour entanglement seems to approximate the maximally entangled mixed states (MEMS). Further we investigate in detail several other properties of the so obtained optimal state.Comment: 12 pages, 4 figures, 2nd version minor change

Pathways for cross-boundary effects of biodiversity on ecosystem functioning

The biodiversity-ecosystem functioning concept asserts that processes in ecosystems are markedly influenced by species richness and other facets of biodiversity. However, biodiversity-ecosystem functioning studies have been largely restricted to single ecosystems, ignoring the importance of functional links - such as the exchange of matter, energy, and organisms - between coupled ecosystems. Here we present a basic concept and outline three pathways of cross boundary biodiversity effects on ecosystem processes and propose an agenda to assess such effects, focusing on terrestrial-aquatic linkages to illustrate the case. This cross-boundary perspective of biodiversity-ecosystem functioning relationships presents a promising frontier for biodiversity and ecosystem science with repercussions for the conservation, restoration, and management of biodiversity and ecosystems from local to landscape scales.Peer reviewe

Beneficial effect of early initiation of lipid-lowering therapy following renal transplantation

Background. Renal transplant recipients have a significantly reduced life expectancy, largely due to premature cardiovascular disease. The aim of the current analysis was to investigate the importance of time of initiation of therapy after transplantation, on the benefits of statin therapy. Methods. 2102 renal transplant recipients with total cholesterol levels of 4.0-9.0 mmol/l were randomly assigned to treatment with fluvastatin (n = 1050) or placebo (n = 1052) and followed for a mean time of 5.1 years. The end-points were major cardiac events. The average median time from transplantation to randomization was 4.5 years (range: 0.5-29 years). Results. In patients starting treatment with fluvastatin 6 years, respectively. The risk reduction for patients initiating therapy with fluvastatin at years 0-2 (compared with >6 years) following transplantation was 59% (RR: 0.41; 95% CI: 0.18-0.92; P = 0.0328). This is also reflected in total time on renal replacement therapy: in patients in the first quartile (120 months) (P = 0.033). Conclusions. Our data support an early introduction of fluvastatin therapy in a population of transplant recipients at high risk of premature coronary heart diseas

Decoherence of entangled kaons and its connection to entanglement measures

We study the time evolution of the entangled kaon system by considering the Liouville - von Neumann equation with an additional term which allows for decoherence. We choose as generators of decoherence the projectors to the 2-particle eigenstates of the Hamiltonian. Then we compare this model with the data of the CPLEAR experiment and find in this way an upper bound on the strength $\lambda$ of the decoherence. We also relate $\lambda$ to an effective decoherence parameter $\zeta$ considered previously in literature. Finally we discuss our model in the light of different measures of entanglement, i.e. the von Neumann entropy $S$, the entanglement of formation $E$ and the concurrence $C$, and we relate the decoherence parameter $\zeta$ to the loss of entanglement: $1 - E$.Comment: comments and references added, 18 pages, 1 figur

Bell inequalities for entangled kaons and their unitary time evolution

We investigate Bell inequalities for neutral kaon systems from Phi resonance decay to test local realism versus quantum mechanics. We emphasize the unitary time evolution of the states, that means we also include all decay product states, in contrast to other authors. Only this guarantees the use of the complete Hilbert space. We develop a general formalism for Bell inequalities including both arbitrary "quasi spin" states and different times; finally we analyze Wigner-type inequalities. They contain an additional term, a correction function h, as compared to the spin 1/2 or photon case, which changes considerably the possibility of quantum mechanics to violate the Bell inequality. Examples for special "quasi spin" states are given, especially those which are sensitive to the CP parameters epsilon and epsilon'.Comment: REVTeX, 22 page

I-MOVE multicentre case–control study 2010/11 to 2014/15 : is there within-season waning of influenza type/subtype vaccine effectiveness with increasing time since vaccination?

Influenza vaccines are currently the best method available to prevent seasonal influenza infection. In most European countries one dose (or two doses for children) of seasonal vaccine is given from September to December to the elderly and other target groups for vaccination. In Europe, influenza seasons can last until mid-May (1), and it is expected that vaccination conveys protection on the individual for the duration of the season. In 13/15 reviewed studies on the length of vaccine-induced protection among the elderly, using anti-haemagglutination antibody titres as a proxy for seroprotection levels, seroprotection rates lasted at least >4 months after vaccination (2). However in the 2011-12 influenza season various studies in Europe reported a decrease in influenza vaccine effectiveness (VE) against A(H3N2) over time within the season (3–5). In the United States, a decrease in VE against A(H3N2) with time since vaccination was suggested in the 2007-8 influenza season (6). The observed decrease of VE over time can be explained by viral change (notably antigenic drift) occurring in the season. Drift in B viruses may be slower than in A viruses (7), and A(H3N2) viruses undergo antigenic drift more frequently than A(H1N1)pdm09 viruses (8). The decrease of VE over time can also be explained by a waning of the immunity conferred by the vaccine independently from viral changes. If vaccine-induced protection wanes more rapidly during the season, then depending on the start and duration of the influenza season, the decline of VE may cause increases in overall incidence, hospitalisations and deaths. Changes to vaccination strategies (timing and boosters) may be needed. As anti-haemagglutination antibody titres are not well defined as a correlate of protection (9,10), vaccine efficacy (as measured in trials) or vaccine effectiveness observational studies may be one way to measure vaccine-induced protection. These studies require a large sample size to model VE by time since vaccination and currently, most of the seasonal observational studies lack the precision required to provide evidence for waning immunity. In this study we pooled data across five post-pandemic seasons (2010/11-2014/15) from the I-MOVE (Influenza - Monitoring Vaccine Effectiveness) multicentre case control studies (1,3,11,12), to obtain a greater sample size to study the effects of time since vaccination on influenza type/subtype-specific VE. We measure influenza type/subtype-specific VE by time since vaccination for the overall season, but also in the early influenza phase; under the hypothesis that virological changes are fewer in the early season, but waning of the vaccine effect should be present regardless of time within the influenza phase

Confirmation of the Chinese version of the Problematic Internet Use Questionnaire Short Form (PIUQ-SF)

Although an increasing number of studies have focused on problematic Internet use, numerous measures exist with only modest investigation into their reliability and validity, both within and across different cultures (Király et al. 2015). The most frequently used questionnaire is the Internet Addiction Test (Young 1998). However, its factor structure appears to be unstable (e.g., Fernandez-Villa et al. 2015; Pontesetal. 2014). Koronczai et al. (2011) suggested that a suitable measure should fit six basic requirements. More specifically, it should be (i) comprehensive, that is examining more, possibly all, aspects of problematic Internet use; (ii) as concise as possible, in order to be able to assess the more impulsive population, and to use in time-limited surveys (or having different forms with different length). Such a measure should be (iii) reliable and valid for different methods of data collection (e.g. online, paper-and-pencil self-rating, face-to-face); as well as (iv) for different age groups (e.g. adolescents and adults), and (v) in different cultural settings. Finally, (vi) cut-off scores should be defined on the basis of clinical examination. The Problematic Internet Use Questionnaire (PIUQ) (Demetrovics et al. 2008) fulfills several of the aforementioned criteria. The 18-item PIUQ is a comprehensive measure that assesses three basic aspects of problematic Internet use (i.e., obsession, neglect, and control disorder). The 18-item and the shorter 9-item versions of the PIUQ have a reliable structure, and are suitable for time-limited surveys and have proved to be valid across various methods of data collection (i.e., online as well as paper-pencil) and age groups (Demetrovics et al. 2008; Koronczai et al. 2011). However, its implementation has so far mostly taken place in Western individualistic cultures (Kelley and Gruber 2010, 2013; Koronczai et al. 2011; Zahodne et al. 2011. To date, the PIUQ has also been applied in a survey in Iran with good Cronbach’s alpha coefficients and subscales (Mazhari 2012a, 2012b). Consequently, validation of the questionnaire in a culture that is significantly different from the Western culture is needed. Accordingly, the aim of the present study was the validation of the 9-item PIUQ among the Chinese population

Post-Transcriptional Regulation of 5-Lipoxygenase mRNA Expression via Alternative Splicing and Nonsense-Mediated mRNA Decay

5-Lipoxygenase (5-LO) catalyzes the two initial steps in the biosynthesis of leukotrienes (LT), a group of inflammatory lipid mediators derived from arachidonic acid. Here, we investigated the regulation of 5-LO mRNA expression by alternative splicing and nonsense-mediated mRNA decay (NMD). In the present study, we report the identification of 2 truncated transcripts and 4 novel 5-LO splice variants containing premature termination codons (PTC). The characterization of one of the splice variants, 5-LOΔ3, revealed that it is a target for NMD since knockdown of the NMD factors UPF1, UPF2 and UPF3b in the human monocytic cell line Mono Mac 6 (MM6) altered the expression of 5-LOΔ3 mRNA up to 2-fold in a cell differentiation-dependent manner suggesting that cell differentiation alters the composition or function of the NMD complex. In contrast, the mature 5-LO mRNA transcript was not affected by UPF knockdown. Thus, the data suggest that the coupling of alternative splicing and NMD is involved in the regulation of 5-LO gene expression