11,553 research outputs found
Isolation of Nuclei from Physarum flavicomum: Demonstration of Nuclear Cyclic Acid AMP Phosphodiesterase
Cyclic AMP phosphodiesterase activity in the nucleus of the myxomycete Physarum flavicomum was demonstrated by cytochemical staining utilizing electron microscopy and by enzymatic assays with tritiated cyclic AMP as the substrate. Cytochemical staining showed Physarum\u27s plasmodial phosphodiesterase activity to be located in the nucleus, along the plasma membrane, in vesicles, and free in the cytoplasm. Nuclear phosphodiesterase, which may be cell cycle dependent, was primarily located in the nucleolus. Nuclei from three to five day old microplasmodial cultures were isolated by the method of Henney and Yee. Whole cells were collected through centrifugation and washed. Pellets were homogenized in a medium composed of 0.01 MTris-HC1 (pH 7.2 at 4 °C), 0.25 M sucrose, 0.01% Triton X-100, and 5mM CaC1â. Nuclei were collected through double filtration and two 1.0 M sucrose density gradient centrifugations. After the nuclei were washed, microscopic examination revealed a purity of over 90%. Radioactive assays of the nuclear preparations demonstrated phosphodiesterase activity consistant with that indicated by cytochemical localization. The specific activity of the nuclear enzyme was 15 nMole of cyclic AMP hydrolyzed /min/mg. of protein
Field Induced Nodal Order Parameter in the Tunneling Spectrum of YBaCuO Superconductor
We report planar tunneling measurements on thin films of
YBaCuO at various doping levels under magnetic fields. By
choosing a special setup configuration, we have probed a field induced energy
scale that dominates in the vicinity of a node of the d-wave superconducting
order parameter. We found a high doping sensitivity for this energy scale. At
Optimum doping this energy scale is in agreement with an induced
order parameter. We found that it can be followed down to low fields at optimum
doping, but not away from it.Comment: 9 pages, 8 figures, accepted for publication in Phys. Rev.
Avoidability of formulas with two variables
In combinatorics on words, a word over an alphabet is said to
avoid a pattern over an alphabet of variables if there is no
factor of such that where is a
non-erasing morphism. A pattern is said to be -avoidable if there exists
an infinite word over a -letter alphabet that avoids . We consider the
patterns such that at most two variables appear at least twice, or
equivalently, the formulas with at most two variables. For each such formula,
we determine whether it is -avoidable, and if it is -avoidable, we
determine whether it is avoided by exponentially many binary words
Vector magnetic hysteresis of hard superconductors
Critical state problems which incorporate more than one component for the
magnetization vector of hard superconductors are investigated. The theory is
based on the minimization of a cost functional
which weighs the changes of the magnetic field vector within the sample. We
show that Bean's simplest prescription of choosing the correct sign for the
critical current density in one dimensional problems is just a particular
case of finding the components of the vector . is
determined by minimizing under the constraint , with a bounded set. Upon the selection of
different sets we discuss existing crossed field measurements and
predict new observable features. It is shown that a complex behavior in the
magnetization curves may be controlled by a single external parameter, i.e.:
the maximum value of the applied magnetic field .Comment: 10 pages, 9 figures, accepted in Phys. Rev.
Metallicities of M Dwarf Planet Hosts from Spectral Synthesis
We present the first spectroscopic metallicities of three M dwarfs with known
or candidate planetary mass companions. We have analyzed high resolution, high
signal-to-noise spectra of these stars which we obtained at McDonald
Observatory. Our analysis technique is based on spectral synthesis of atomic
and molecular features using recently revised cool-star model atmospheres and
spectrum synthesis code. The technique has been shown to yield results
consistent with the analyses of solar-type stars and allows measurements of M
dwarf [M/H] values to 0.12 dex precision. From our analysis, we find [M/H] =
-0.12, -0.32, and -0.33 for GJ 876, GJ 436, and GJ 581 respectively. These
three M dwarf planet hosts have sub-solar metallicities, a surprising departure
from the trend observed in FGK-type stars. This study is the first part of our
ongoing work to determine the metallicities of the M dwarfs included in the
McDonald Observatory planet search program.Comment: 13 pages, 2 figures, accepted for publication in ApJ
The dynamical viability of scalar-tensor gravity theories
We establish the dynamical attractor behavior in scalar-tensor theories of
dark energy, providing a powerful framework to analyze classes of theories,
predicting common evolutionary characteristics that can be compared against
cosmological constraints. In the Jordan frame the theories are viewed as a
coupling between a scalar field, \Phi, and the Ricci scalar, R, F(\Phi)R. The
Jordan frame evolution is described in terms of dynamical variables m \equiv
d\ln F/d\ln \Phi and r \equiv -\Phi F/f, where F(\Phi) = d f(\Phi)/d\Phi. The
evolution can be alternatively viewed in the Einstein frame as a general
coupling between scalar dark energy and matter, \beta. We present a complete,
consistent picture of evolution in the Einstein and Jordan frames and consider
the conditions on the form of the coupling F and \beta required to give the
observed cold dark matter (CDM) dominated era that transitions into a late time
accelerative phase, including transitory accelerative eras that have not
previously been investigated. We find five classes of evolutionary behavior of
which four are qualitatively similar to those for f(R) theories (which have
\beta=1/2). The fifth class exists only for |\beta| < \sqrt{3}/4, i.e. not for
f(R) theories. In models giving transitory late time acceleration, we find a
viable accelerative region of the (r,m) plane accessible to scalar-tensor
theories with any coupling, \beta (at least in the range |\beta| \leq 1/2,
which we study in detail), and an additional region open only to theories with
|\beta| < \sqrt{3}/4.Comment: 24 pages, 3 figure
3D simulations of M star atmosphere velocities and their influence on molecular FeH lines
We present an investigation of the velocity fields in early to late M-type
star hydrodynamic models, and we simulate their influence on FeH molecular line
shapes. The M star model parameters range between log g of 3.0 - 5.0 and Teff
of 2500 K and 4000 K. Our aim is to characterize the Teff- and log g
-dependence of the velocity fields and express them in terms of micro- and
macro-turbulent velocities in the one dimensional sense. We present also a
direct comparison between 3D hydrodynamical velocity fields and 1D turbulent
velocities. The velocity fields strongly affect the line shapes of FeH, and it
is our goal to give a rough estimate for the log g and Teff parameter range in
which 3D spectral synthesis is necessary and where 1D synthesis suffices. In
order to calculate M-star structure models we employ the 3D
radiative-hydrodynamics (RHD) code CO5BOLD. The spectral synthesis on these
models is performed with the line synthesis code LINFOR3D. We describe the 3D
velocity fields in terms of a Gaussian standard deviation and project them onto
the line of sight to include geometrical and limb-darkening effects. The micro-
and macro-turbulent velocities are determined with the "Curve of Growth" method
and convolution with a Gaussian velocity profile, respectively. To characterize
the log g and Teff dependence of FeH lines, the equivalent width, line width,
and line depth are regarded. The velocity fields in M-stars strongly depend on
log g and Teff. They become stronger with decreasing log g and increasing Teff.Comment: 14 pages, 17 figures, 3 tables, accepted by Astronomy & Astrophysic
Chronic Pancreatitis and Neoplasia: Correlation or Coincidence
Any link between pancreatic carcinoma and chronic pancreatitis could reflect the malignant potential of a chronic inflammatory process. Four patients with ductal adenocarcinomas had a long history of pancreatic pain (median duration 5 years) and showed clearcut evidence of chronic pancreatitis âdownstreamâ of the tumour. Four were alcoholics and two heavy smokers. These four cases arose within a surgical series of approximately 250 patients with chronic pancreatitis, giving an incidence of 1.6 per cent. The incidence and anatomical distribution of carcinoma and chronic pancreatitis could possibly be consistent with a casual relationship
The Transiting Exoplanet Community Early Release Science Program for JWST
The James Webb Space Telescope (JWST) presents the opportunity to transform our understanding of planets and the origins of life by revealing the atmospheric compositions, structures, and dynamics of transiting exoplanets in unprecedented detail. However, the high-precision, timeseries observations required for such investigations have unique technical challenges, and prior experience with Hubble, Spitzer, and other facilities indicates that there will be a steep learning curve when JWST becomes operational. In this paper, we describe the science objectives and detailed plans of the Transiting Exoplanet Community Early Release Science (ERS) Program, which is a recently approved program for JWST observations early in Cycle 1. We also describe the simulations used to establish the program. The goal of this project, for which the obtained data will have no exclusive access period, is to accelerate the acquisition and diffusion of technical expertise for transiting exoplanet observations with JWST, while also providing a compelling set of representative data sets that will enable immediate scientific breakthroughs. The Transiting Exoplanet Community ERS Program will exercise the timeseries modes of all four JWST instruments that have been identified as the consensus highest priorities, observe the full suite of transiting planet characterization geometries (transits, eclipses, and phase curves), and target planets with host stars that span an illustrative range of brightnesses. The observations in this program were defined through an inclusive and transparent process that had participation from JWST instrument experts and international leaders in transiting exoplanet studies. The targets have been vetted with previous measurements, will be observable early in the mission, and have exceptional scientific merit. Community engagement in the project will be centered on a two-phase Data Challenge that culminates with the delivery of planetary spectra, timeseries instrument performance reports, and open-source data analysis toolkits in time to inform the agenda for Cycle 2 of the JWST mission
Delayed Recombination and Standard Rulers
Measurements of Baryonic Acoustic Oscillations in galaxy surveys have been
recognized as a powerful tool for constraining dark energy. However, this
method relies on the knowledge of the size of the acoustic horizon at
recombination derived from Cosmic Microwave Background Anisotropy measurements.
This estimate is typically derived assuming a standard recombination scheme;
additional radiation sources can delay recombination altering the cosmic
ionization history and the cosmological inferences drawn from CMB and BAO data.
In this paper we quantify the effect of delayed recombination on the
determination of dark energy parameters from future BAO surveys such as BOSS
and WFMOS. We find the impact to be small but still not negligible. In
particular, if recombination is non-standard (to a level still allowed by CMB
data), but this is ignored, future surveys may incorrectly suggest the presence
of a redshift dependent dark energy component. On the other hand, in the case
of delayed recombination, adding to the analysis one extra parameter describing
deviations from standard recombination, does not significantly degrade the
error-bars on dark energy parameters and yields unbiased estimates.Comment: 8 pages, 5 figure
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