Electron probe analysis of cementum surfaces

Bu araştırmada sağlıklı, periodontal hastalıklı ve kök yüzeyi düzleştirmesi yapılmış hastalıklı sement yüzeylerinin mineral içeriklerinin sapta¬ması amaçlanmıştır. Mineralize dokunun lokalize bölgelerinin inorganik element içeriğini tayin etmekte elektron prob analiz daha uygun bir tekniktir. Bu nedenle Elektron Prob cihazında bu yüzeylerin Ca ve P içerikleri tayin edilmiş, yanısıra yüzeylerin morfolojik özellikleri de ince¬lenmiştir. Bulgularımız hastalıklı sement yüzeyinin Ca ve P içeriğinin arttığını göstermiştir. Kök yüzeyi düzleştirmesi sonrasında hastalıklı sement yüzeyinin mineral içeriğinin azaldığı, ancak bu farkın istatistiksel anlam taşımadığı belirlenmiştir. Elde ettiğimiz sonuçlar, periodontal hastalıklı sement yüzeyinde inorganik yapının değiştiğini, kök yüzeyi düzleştirmesi ile hastalıklı sementin tamamen kaldırılmasının hipermineralize tabaka tarafından önlendiğini ortaya koymuştur.The purpose of this study was to determine the presence ofcertai n inorganic elements in various cemen¬tum surfaces. The electron probe analysis appears to be the most suitable technique for examining elemen¬tal composition within localized small areas of mineralized tissue, it was used to determine the mineral con¬tents of nondiseased, periodontally diseased and root planing applied diseased cementum surfaces were determined by electron probe. Calcium (Ca) and phosphorus (P) were the elements searched for. Surface morphological features of these cementum surfaces were also examined. Electron probe analysis indicated an elevated Ca and P content in diseased cementum surfaces. Root planing of these surfaces did not reduce the mineral contents of these root surfaces. Our findings indicate that on the cementum surfaces affected by periodontal disease inorganic contents were altered and the hypermineralized layer in diseased cementum may play a role in preventing total diseased cementum removal by root planing

Examination of the effects of various orthodontic appliances on periodontal tissues [Ceşitli ortodontik aparey taşiyan bireylerin periodontal dokularinin incelenmesi.]

PubMed ID: 2101651This study has been planned to examine the effects of various orthodontic appliances on periodontal tissues and the effect of motivation from the point of oral hygiene during the usage period of these appliances. 45 individuals; 15 of them using removable appliances, 15 of them using fixed-DBS appliances and 15 of the patients treating with fixed-multiband technics, have been used. In the beginning; the pocket depths, gingival and plaque indices of all cases have been reported and then they were motivated for their oral hygiene. The measurements were repeated for each of the motivated cases after 1 month. Statistically significant difference was found only in pocket depth between the 3 groups. There were statistically significant differences in pocket depth, gingival and plaque indices values in each of the groups before and after motivation

Effect of various demineralizing agents on mineral contents of cementum surfaces (an electron probe analysis).

PubMed ID: 9569807The purpose of this investigation was to determine the surface morphology and mineral content of periodontally diseased cementum surfaces following root planing and conditioning with either citric acid, tetracycline HCL and minocycline HCL. Mineral contents of cementum surfaces were determined with an electron probe. All 3 demineralizing agents caused the exposure of the collagen matrix on the root surface but citric acid was the more effective. Calcium (Ca) and phosphorus (P) showed higher values in citric acid and minocycline HCL treated cementum surfaces compared to nondiseased cementum surfaces. The Ca and P were found to be in similar proportions in the tetracycline HCL treated group and nondiseased cementum surfaces. Although citric acid is a strong demineralizing agent, our specimens' cementum surfaces to which citric acid was treated showed high Ca and P content. In our opinion this difference about the effects of demineralizing agents on the surface morphology and surface element content was found to be related to the facts that whether the total amount of hypermineralized cementum as removed or not with root planing and the lacking criteria to detects the amount of this removal. Our findings suggest that these demineralizing agents can demineralize cementum surface after root planing but can not remove all of the hypermineralized diseased cementum. For this reason, it may be stated that it is more important to remove the pathologically altered cementum than applying various chemical agents

The treatment of a localized osseous sequestrum with porous bone mineral in combination with a collagen membrane and resorbable bone pins.

PubMed ID: 2000134006The ultimate goal of periodontal therapy should not be limited to the establishment and maintenance of periodontal health. The potential regeneration of the hard and soft periodontal tissues lost due to disease also should be considered. This case presentation evaluated a new surgical technique for the treatment of a variety of localized bone defects, utilizing porous bone mineral in combination with collagen membrane and resorbable bone pins

Effectiveness of periodontal therapy in patients with drug-induced gingival overgrowth. Long-term results

WOS: 000082644500003PubMed ID: 10505798Background: Drug-induced gingival overgrowth (DGO) is one of the well-recognized side effects of cyclosporin A (CsA) or nifedipine (Ni). After surgical periodontal therapy, the incidence of DGO recurrence is not known. The aim of this study was to evaluate the results of surgical periodontal therapy in patients receiving CsA or Ni and who exhibit severe long-term DGO. In addition, the relationship between various variables and the recurrence of severe DGO after periodontal surgery was investigated. Methods: A total of 38 patients, 22 with CsA-induced DGO and 16 with Ni-induced DGO, were included in this study. At baseline, patients received initial periodontal therapy, after which either the upper or lower anterior segment in each patient was surgically treated. Surgical periodontal therapy consisted of the flap technique with a 90 degrees gingivectomy incision. Following surgery, patients were placed on a maintenance therapy recall program and were monitored for 18 months. Patients were seen once a month for the first 3 months and once every 3 months for the following 15 months, Plaque index (PI), papilla bleeding index (PBI) and DGO scores in the treated segments were recorded at each recall appointment. Attendance at recall appointments was also noted for each patient. Results: Recurrence of severe DGO was observed in 13 of the 38 patients (34%) 18 months following periodontal surgery. Multiple regression analysis indicated that age, gingival inflammation, and attendance at recall appointments were significant determinants of the recurrence of severe DGO. Conclusions: This study suggests that regular re-motivation and professional care at frequent recall appointments after periodontal surgery are of great importance in patients receiving CsA or Ni. By maintaining periodontal health, the life quality of these patients may be raised

Effects of selective cyclooxygenase-2 inhibition on gingival tissue levels of prostaglandin E-2 and prostaglandin F2 alpha and clinical parameters of chronic periodontitis

WOS: 000187314000008PubMed ID: 12593597Background: The purpose of the present study was to evaluate the effect of a relatively selective cyclooxygenase (COX)-2 inhibitor (nimesulide) and non-selective COX-1/COX-2 inhibitor (naproxen) used as an adjunct to non-surgical (scaling and root planing [SRP]) periodontal therapy in chronic periodontitis patients on the gingival tissue (GT) levels of prostaglandin (PG)E-2 and PGF(2alpha). Methods: Thirty patients with chronic periodontitis were divided into 3 groups of 10 each. One group received 100 mg of nimesulide; one received 275 mg of naproxen sodium; and the third group received placebo tablets in a 2 x 1 regimen for 10 days as an adjunct to SRP. GT samples were obtained before drug intake and on day 10. Plaque index (PI) and papillary bleeding index (PBI) scores were recorded at baseline, day 10, and at 3 months; probing depth (PD) and clinical attachment level (CAL) were recorded at baseline and at 3 months. The levels of PGE(2) were detected using an enzyme immunoassay (EIA), and the levels of PGF(2alpha) were analyzed by radioimmunoassay (RIA). Differences among and within the groups were assessed using non-parametric statistical analysis. Ten periodontally healthy individuals served as controls. Results: All 3 groups showed statistically significant reductions in PBI and Pl on day 10 and at 3 months (P0.05), while a significant increase was observed in the placebo group (P<0.05) on day 10. Both the nimesulide and naproxen groups showed a significant decrease (P<0.05) in PGF(2alpha) level, while the placebo group showed a significant increase (P<0.05). Conclusions: Nimesulides, relatively selective COX-2 inhibitors, may have additional inhibitory effects on GT PGF(2alpha) levels in the first week following non-surgical periodontal treatment. However, nimesulide has an insignificant effect on reducing PGE(2) levels in gingival tissue. The determination of GT levels of COX-1 and COX-2 enzymes as well as PGE(2) and PGF(2alpha) in long-term studies may provide further support for the adjunctive use of selective COX-2 inhibitors in treatment of chronic periodontitis

Prepubertal periodontitis associated with chronic granulomatous disease

WOS: 000168807700013PubMed ID: 11350528Background: Generalised prepubertal periodontitis is a rare entity that is usually a consequence of severe systemic diseases. Chronic granulomatous disease is one of the extremely rare inherited immunodeficiency diseases, which predisposes the patient to recurrent severe bacterial and fungal infections. Aim: The purpose of this report is to describe a 5-year old male patient suffering from prepubertal periodontitis associated with chronic granulomatous disease, who was referred to the Department of Periodontology for treatment of severe gingival inflammation. Methods: A detailed past history was obtained and thorough clinical and laboratory examinations were performed. Results: Medical tests revealed the only immunodeficiency sign as the extremely low burst test result. The patient was diagnosed as having an autosomal recessive (AR) form of chronic granulomatous disease. He was put an prophylactic treatment with trimethoprim-sulfamethoxazole (TMP-SMX) and also a periodontal maintenance regimen with regular I-month intervals. Conclusion: This case report emphasises the importance of the differential diagnosis of severe immunodeficiency in the background of prepubertal periodontitis