27 research outputs found
Efeito do citrato e taurina em meio CR2aa no desenvolvimento de embriões bovinos fecundados in vitro
Desenvolvimento de embriões de camundongas após manutenção em diferentes soluções de manipulação
Simultaneous assessment of plasmatic, acrosomal, and mitochondrial membranes in ram sperm by fluorescent probes
242 DEVELOPMENT OF BAC FISH PROBES FOR GENETIC ANALYSIS OF NON-HUMAN PRIMATE GAMETES AND EMBRYONIC STEM CELLS
162INSULIN IMPROVES FREQUENCY OF CLEAVAGE AND IN VITRO DEVELOPMENT OF BOVINE EMBRYOS IN DEFINED MEDIUM
Effect of Age and Breeding Season on the Developmental Capacity of Oocytes from Unstimulated and Follicle-Stimulating Hormone-Stimulated Rhesus Monkeys1
Inner Cell Mass Localization of NANOG Precedes OCT3/4 in Rhesus Monkey Blastocysts
The mechanism by which the inner cell mass (ICM) and trophectoderm (TE) become specified is poorly understood. Considerable species variation is evident in the expression of lineage-specific and embryonic stem cell (ESC) regulatory markers. We sought to investigate localization patterns of these markers in rhesus macaque compact morulae and blastocysts. NANOG protein was restricted to the ICM of blastocysts. In contrast to a previous report, the expression of CDX2 was detected in the primate blastocyst, localized specifically to the TE. Unlike the mouse embryo, OCT4 protein was detected using two different antibodies in both the ICM and TE. The ubiquitous pattern of OCT4 expression is consistent with observations in human, cow, and pig embryos. Significantly, lack of restricted OCT4 protein, and ICM localization of NANOG in primate blastocysts, suggests that NANOG may determine inner cell mass fate more specifically during primate development or may be less susceptible to culture artifacts. These results contrast markedly with current mechanistic hypotheses, although other factors may lie upstream of NANOG to constitute a complex interactive network. This difference may also underlie observations that regulatory mechanisms in ESC differ between mice and primates