288 research outputs found
PARTIAL PURIFICATION AND IMMUNO-BIOCHEMICAL CHARACTERISATION OF FERTILITY ASSOCIATED PROTEIN OF KARAN FRIES BULL SEMINAL PLASMA
The objective of the present study was detection, isolation, partial purification and
immunobiochemical characterization of fertility associated protein in the seminal plasma of high
prolific Karan fries bull. Seminal plasma of Karan Fries bull was partially purified by gel filtration
chromatography and analyzed by 10% SDS-PAGE for their polypeptide profile. PAGE analysis
revealed major band of 55 kDa, and 26 kDa. Hyperimmune serum was raised in rabbit against crude
seminal plasma protein. Single precipitin line was observed in DID test when each of the partially
purified 26 kDa and 55 kDa proteins were reacted with hyperimmune serum. These proteins were
also found to be immunoreactive against hyperimmune serum in Western blot technique
Hieroglyph: Hierarchical Glia Graph Skeletonization and Matching
Automatic 3D reconstruction of glia morphology is a powerful tool necessary
for investigating the role of microglia in neurological disorders in the
central nervous system. Current glia skeleton reconstruction techniques fail to
capture an accurate tracing of the processes over time, useful for the study of
the microglia motility and morphology in the brain during healthy and diseased
states. We propose Hieroglyph, a fully automatic temporal 3D skeleton
reconstruction algorithm for glia imaged via 3D multiphoton microscopy.
Hieroglyph yielded a 21% performance increase compared to state of the art
automatic skeleton reconstruction methods and outperforms the state of the art
in different measures of consistency on datasets of 3D images of microglia. The
results from this method provide a 3D graph and digital reconstruction of glia
useful for a myriad of morphological analyses that could impact studies in
brain immunology and disease.Comment: submitted to IEEE International Conference on Image Processing, 201
Evidence for one-dimensional chiral edge states in a magnetic Weyl semimetal Co<sub>3</sub>Sn<sub>2</sub>S<sub>2</sub>
The physical realization of Chern insulators is of fundamental and practical interest, as they are predicted to host the quantum anomalous Hall (QAH) effect and topologically protected chiral edge states which can carry dissipationless current. Current realizations of the QAH state often require complex heterostructures and sub-Kelvin temperatures, making the discovery of intrinsic, high temperature QAH systems of significant interest. In this work we show that time-reversal symmetry breaking Weyl semimetals, being essentially stacks of Chern insulators with inter-layer coupling, may provide a new platform for the higher temperature realization of robust chiral edge states. We present combined scanning tunneling spectroscopy and theoretical investigations of the magnetic Weyl semimetal, Co3Sn2S2. Using modeling and numerical simulations we find that depending on the strength of the interlayer coupling, chiral edge states can be localized on partially exposed kagome planes on the surfaces of a Weyl semimetal. Correspondingly, our dI/dV maps on the kagome Co3Sn terraces show topological states confined to the edges which display linear dispersion. This work provides a new paradigm for realizing chiral edge modes and provides a pathway for the realization of higher temperature QAH effect in magnetic Weyl systems in the two-dimensional limit. © 2021, The Author(s)
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