PARTIAL PURIFICATION AND IMMUNO-BIOCHEMICAL CHARACTERISATION OF FERTILITY ASSOCIATED PROTEIN OF KARAN FRIES BULL SEMINAL PLASMA

The objective of the present study was detection, isolation, partial purification and immunobiochemical characterization of fertility associated protein in the seminal plasma of high prolific Karan fries bull. Seminal plasma of Karan Fries bull was partially purified by gel filtration chromatography and analyzed by 10% SDS-PAGE for their polypeptide profile. PAGE analysis revealed major band of 55 kDa, and 26 kDa. Hyperimmune serum was raised in rabbit against crude seminal plasma protein. Single precipitin line was observed in DID test when each of the partially purified 26 kDa and 55 kDa proteins were reacted with hyperimmune serum. These proteins were also found to be immunoreactive against hyperimmune serum in Western blot technique

Hieroglyph: Hierarchical Glia Graph Skeletonization and Matching

Automatic 3D reconstruction of glia morphology is a powerful tool necessary for investigating the role of microglia in neurological disorders in the central nervous system. Current glia skeleton reconstruction techniques fail to capture an accurate tracing of the processes over time, useful for the study of the microglia motility and morphology in the brain during healthy and diseased states. We propose Hieroglyph, a fully automatic temporal 3D skeleton reconstruction algorithm for glia imaged via 3D multiphoton microscopy. Hieroglyph yielded a 21% performance increase compared to state of the art automatic skeleton reconstruction methods and outperforms the state of the art in different measures of consistency on datasets of 3D images of microglia. The results from this method provide a 3D graph and digital reconstruction of glia useful for a myriad of morphological analyses that could impact studies in brain immunology and disease.Comment: submitted to IEEE International Conference on Image Processing, 201

Faktor snage nanostrukturiranog bizmut telurida određen oblicima

Bismuth telluride is a thermoelectric material with high figure of merit, used for cooling applications at room temperature. To investigate the effect of morphology and grain size on transport parameters, nanostructured bismuth telluride has been synthesized under different reaction conditions and characterized by X-ray diffraction, transmission electron microscopy and scanning electron microscopy. From the measurement of electrical conductivity and thermoelectric power, power factor for different samples has been obtained. The results show that the transport parameters are critically influenced by the morphology and dimension of the samples that in turn depend on the condition of synthesis. The experimentally observed variation of electrical conductivity with the change of dimensionality of the samples from 2D to 0D, which is in line with the theoretical prediction made by other workers, is discussed. There is a variation of the power factor of the samples prepared under different conditions of synthesis.Bizmut telurid je termoelektrična tvar s visokom učinkovitošću koja se rabi za hlađenje na sobnim temperaturama. Radi istraživanja učinka oblika i veličine zrna na transportne parametre, sintetizirali smo nanostrukturirani bizmut telurid u različitim uvjetima i ispitivali rentgenskom difrakcijom, te propusnom i pretražnom elektronskom mikroskopijom. Mjerenjem električne vodljivosti i termoelektrične snage niza uzoraka odredili smo faktore snage. Ishodi analize pokazuju da transportni parametri jako ovise o obliku i veličini uzoraka, koji pak ovise o uvjetima njihove sinteze. Raspravljamo o opaženim promjenama električne vodljivosti ovisnim o dimenzionalnosti uzoraka od 2D do 0D i nalazimo sklad s teorijskim predviđanjima drugih autora. Nalazimo promjene faktora snage uzoraka pripremljenih uz različite uvjete sinteze

Faktor snage nanostrukturiranog bizmut telurida određen oblicima

Bismuth telluride is a thermoelectric material with high figure of merit, used for cooling applications at room temperature. To investigate the effect of morphology and grain size on transport parameters, nanostructured bismuth telluride has been synthesized under different reaction conditions and characterized by X-ray diffraction, transmission electron microscopy and scanning electron microscopy. From the measurement of electrical conductivity and thermoelectric power, power factor for different samples has been obtained. The results show that the transport parameters are critically influenced by the morphology and dimension of the samples that in turn depend on the condition of synthesis. The experimentally observed variation of electrical conductivity with the change of dimensionality of the samples from 2D to 0D, which is in line with the theoretical prediction made by other workers, is discussed. There is a variation of the power factor of the samples prepared under different conditions of synthesis.Bizmut telurid je termoelektrična tvar s visokom učinkovitošću koja se rabi za hlađenje na sobnim temperaturama. Radi istraživanja učinka oblika i veličine zrna na transportne parametre, sintetizirali smo nanostrukturirani bizmut telurid u različitim uvjetima i ispitivali rentgenskom difrakcijom, te propusnom i pretražnom elektronskom mikroskopijom. Mjerenjem električne vodljivosti i termoelektrične snage niza uzoraka odredili smo faktore snage. Ishodi analize pokazuju da transportni parametri jako ovise o obliku i veličini uzoraka, koji pak ovise o uvjetima njihove sinteze. Raspravljamo o opaženim promjenama električne vodljivosti ovisnim o dimenzionalnosti uzoraka od 2D do 0D i nalazimo sklad s teorijskim predviđanjima drugih autora. Nalazimo promjene faktora snage uzoraka pripremljenih uz različite uvjete sinteze